NRP1 Antibody - #DF7877

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Product: NRP1 Antibody
Catalog: DF7877
Description: Rabbit polyclonal antibody to NRP1
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 103 kDa; 103kD(Calculated).
Uniprot: O14786
RRID: AB_2841305

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(91%), Bovine(100%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NRP1 Antibody detects endogenous levels of total NRP1.
RRID:
AB_2841305
Cite Format: Affinity Biosciences Cat# DF7877, RRID:AB_2841305.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A5 protein; BDCA4; BLOOD DENDRITIC CELL ANTIGEN 4; CD304; Neuropilin-1; Neuropilin1; NP1; NPN1; NRP 1; NRP; NRP1; NRP1_HUMAN; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165R;

Immunogens

Immunogen:
Uniprot:

O14786(NRP1_HUMAN) >>Visit HPA database.

Gene(ID):
NRP1(8829)
Expression:
O14786 NRP1_HUMAN:

The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.

Sequence:
MERGLPLLCAVLALVLAPAGAFRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFVPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSEFPTYGFNCEFGWGSHKTFCHWEHDNHVQLKWSVLTSKTGPIQDHTGDGNFIYSQADENQKGKVARLVSPVVYSQNSAHCMTFWYHMSGSHVGTLRVKLRYQKPEEYDQLVWMAIGHQGDHWKEGRVLLHKSLKLYQVIFEGEIGKGNLGGIAVDDISINNHISQEDCAKPADLDKKNPEIKIDETGSTPGYEGEGEGDKNISRKPGNVLKTLDPILITIIAMSALGVLLGAVCGVVLYCACWHNGMSERNLSALENYNFELVDGVKLKKDKLNTQSTYSEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Pig
91
Sheep
91
Rabbit
91
Chicken
64
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14786 As Substrate

Site PTM Type Enzyme
T30 Phosphorylation
S47 Phosphorylation
S51 Phosphorylation
K53 Ubiquitination
K106 Ubiquitination
N150 N-Glycosylation
K166 Ubiquitination
N261 N-Glycosylation
S267 Phosphorylation
Y297 Phosphorylation
Y310 Phosphorylation
T316 Phosphorylation
S321 Phosphorylation
Y322 Phosphorylation
Y353 Phosphorylation
Y354 Phosphorylation
Y358 Phosphorylation
S364 Phosphorylation
S432 Phosphorylation
T448 Phosphorylation
N522 N-Glycosylation
S524 Phosphorylation
S612 O-Glycosylation
T827 O-Glycosylation
T827 Phosphorylation
S829 O-Glycosylation
T830 O-Glycosylation
S844 O-Glycosylation
S894 Phosphorylation
Y899 Phosphorylation
K908 Ubiquitination
K913 Ubiquitination
T919 Phosphorylation
Y920 Phosphorylation
S921 Phosphorylation

Research Backgrounds

Function:

Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR.

Binds VEGF-165 and may inhibit its binding to cells. May induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.

Subcellular Location:

Secreted.

Mitochondrion membrane>Single-pass type I membrane protein. Cell membrane>Single-pass type I membrane protein. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.

Subunit Structure:

Homodimer, and heterodimer with NRP2. Interacts with FER (By similarity). Interacts with PLXNB1. Interacts with VEGFA. Interacts with ABCB8/MITOSUR in mitochondria.

Family&Domains:

The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding.

Belongs to the neuropilin family.

Research Fields

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Development > Axon guidance.   (View pathway)

References

1). Neuropilin-1 Interacts with Fibronectin-1 to Promote Epithelial–Mesenchymal Transition Progress in Gastric Cancer. OncoTargets and Therapy, 2020 (PubMed: 33116644) [IF=4.0]

Application: WB    Species: human    Sample: gastric cancer cells

Figure 3 |Establishment of NRP1 overexpression and knockdown cell lines (A) Analysis of NRP1 expression in different cell lines using Western blot.

Application: WB    Species: Human    Sample: gastric cancer tissue

Figure 1 NRP1 highly expressed in gastric cancer and predicts poor prognosis. (A–B) Survival curve of high NRP1 expression in patients with gastric cancer. (C) According to the TCGA database, high expression of NRP1 in gastric cancer tissues compared with normal tissues. (D) The high expression of NRP1 in metastatic gastric cancer. (E) Expression of NRP1 in gastric cancer patients with different pathological grade. (F) Expression of NRP1 in gastric cancer patients of different clinical stage. (G) Protein levels of NRP1 in ten pairs of fresh tumor tissues and adjacent normal tissues. Differences were considered significant at P < 0.05 and labeled with *.

Application: IHC    Species: Human    Sample: gastric cancer cell

Figure 3 Establishment of NRP1 overexpression and knockdown cell lines (A) Analysis of NRP1 expression in different cell lines using Western blot. (B) Detection of protein levels of NRP1 in gastric cancer cell lines after NRP1 overexpression of knockdown. (C) Wound healing was used to detect cell migration after ectopic alteration of NRP1 expression. (D) Transwell was performed to detect cell invasion after ectopic alteration of NRP1 expression. (E) Cell proliferation was analyzed by colony formation. (F) Protein levels of E-cadherin, N-cadherin and Vimentin were detected by Western blot. Differences were considered significant at P < 0.05 and labeled with *

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