NDUFAF1 Antibody - #DF7870
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF7870, RRID:AB_2841299.
Fold/Unfold
CGI 65; CGI65; CIA30; CIA30_HUMAN; Complex I intermediate associated protein 30; Complex I intermediate associated protein 30 mitochondrial; Complex I intermediate-associated protein 30, mitochondrial; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 1; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1; NADH:ubiquinone oxidoreductase complex assembly factor 1; NDUFAF 1; Ndufaf1;
Immunogens
- Q9Y375 CIA30_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALVHKLLRGTYFLRKFSKPTSALYPFLGIRFAEYSSSLQKPVASPGKASSQRKTEGDLQGDHQKEVALDITSSEEKPDVSFDKAIRDEAIYHFRLLKDEIVDHWRGPEGHPLHEVLLEQAKVVWQFRGKEDLDKWTVTSDKTIGGRSEVFLKMGKNNQSALLYGTLSSEAPQDGESTRSGYCAMISRIPRGAFERKMSYDWSQFNTLYLRVRGDGRPWMVNIKEDTDFFQRTNQMYSYFMFTRGGPYWQEVKIPFSKFFFSNRGRIRDVQHELPLDKISSIGFTLADKVDGPFFLEIDFIGVFTDPAHTEEFAYENSPELNPRLFK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y375 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S74 | Phosphorylation | Uniprot | |
| Y92 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| Y200 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | Uniprot |
Research Backgrounds
Chaperone protein involved in early stages of the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
Mitochondrion. Mitochondrion matrix.
Note: Peripherally associated with the matrix face of the mitochondrial inner membrane.
Ubiquitous.
Part of the mitochondrial complex I assembly (MCIA) complex. The complex comprises at least TMEM126B, NDUFAF1, ECSIT, and ACAD9 (By similarity). Interacts with ECSIT. Interacts with ACAD9. At early stages of complex I assembly, it is found in intermediate subcomplexes that contain different subunits including NDUFB6, NDUFA6, NDUFA9, NDUFS3, NDUFS7, ND1, ND2 and ND3.
Belongs to the CIA30 family.
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