GSTK1 Antibody | Affinity Biosciences

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Western blot analysis of GSTK1 using Jurkat whole lysates.

Product:	GSTK1 Antibody
Catalog:	DF7869
Description:	Rabbit polyclonal antibody to GSTK1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	25 kDa; 25kD(Calculated).
Uniprot:	Q9Y2Q3
RRID:	AB_2841298

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Zebrafish(80%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%)

Clonality:

Polyclonal

Specificity:

GSTK1 Antibody detects endogenous levels of total GSTK1.

RRID:

AB_2841298
Cite Format: Affinity Biosciences Cat# DF7869, RRID:AB_2841298.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EC 2.5.1.18; Glutathione S transferase subunit 13; Glutathione S-transferase k1; Glutathione S-transferase kappa 1; Glutathione S-transferase subunit 13; Glutathione S-transferase subunit 13 homolog; GST 13 13; GST 13-13; GST; GST class kappa; GST class-kappa; GST13; GST13-13; GSTK1 1; Gstk1; GSTK1-1; GSTK1_HUMAN; hGSTK1;

Immunogens

Immunogen:

Uniprot:

Q9Y2Q3(GSTK1_HUMAN) >>Visit HPA database.

Gene(ID):

GSTK1(373156)

Expression:

Q9Y2Q3 GSTK1_HUMAN:

Ubiquitous.

Sequence:

Q9Y2Q3 GSTK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGPLPRTVELFYDVLSPYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAMRFLTAVNLEHPEMLEKASRELWMRVWSRNEDITEPQSILAAAEKAGMSAEQAQGLLEKIATPKVKNQLKETTEAACRYGAFGLPITVAHVDGQTHMLFGSDRMELLAHLLGEKWMGPIPPAVNARL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Rabbit

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2Q3 As Substrate

Q9Y2Q3

Site	PTM Type	Source
K62	Ubiquitination	Uniprot
Y65	Phosphorylation	Uniprot
K71	Acetylation	Uniprot
K71	Ubiquitination	Uniprot
S89	Phosphorylation	Uniprot
S96	Phosphorylation	Uniprot
S98	Phosphorylation	Uniprot
T104	Phosphorylation	Uniprot
K116	Acetylation	Uniprot
K116	Ubiquitination	Uniprot
K144	Acetylation	Uniprot
S148	Phosphorylation	Uniprot
K158	Acetylation	Uniprot
K158	Ubiquitination	Uniprot
T161	Phosphorylation	Uniprot
K165	Acetylation	Uniprot
K169	Acetylation	Uniprot
K169	Ubiquitination	Uniprot

Research Backgrounds

Q9Y2Q3_GSTK1_HUMAN

Function:

Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).

Subcellular Location:

Peroxisome.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the GST superfamily. Kappa family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

Restrictive clause

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For Research Use Only.
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GSTK1 Antibody - #DF7869