F13A1 Antibody - #DF7861

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Product: F13A1 Antibody
Catalog: DF7861
Description: Rabbit polyclonal antibody to F13A1
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 83 kDa; 83kD(Calculated).
Uniprot: P00488
RRID: AB_2841292

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(82%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
F13A1 Antibody detects endogenous levels of total F13A1.
RRID:
AB_2841292
Cite Format: Affinity Biosciences Cat# DF7861, RRID:AB_2841292.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bA525O21.1 (coagulation factor XIII, A1 polypeptide); Coagulation factor XIII A chain; Coagulation factor XIII A1 polypeptide; Coagulation factor XIII A1 subunit; Coagulation factor XIII, A polypeptide; Coagulation factor XIIIa; F13A; F13A_HUMAN; F13a1; Factor XIIIA; Fibrin stabilizing factor, A subunit; Fibrinoligase; FSF, A subunit; Protein glutamine gamma glutamyltransferase A chain; Protein-glutamine gamma-glutamyltransferase A chain; TGase; Transglutaminase A chain; Transglutaminase, plasma; Transglutaminase. plasma;

Immunogens

Immunogen:
Uniprot:

P00488(F13A_HUMAN) >>Visit HPA database.

Gene(ID):
F13A1(2162)
Sequence:
MSETSRTAFGGRRAVPPNNSNAAEDDLPTVELQGVVPRGVNLQEFLNVTSVHLFKERWDTNKVDHHTDKYENNKLIVRRGQSFYVQIDFSRPYDPRRDLFRVEYVIGRYPQENKGTYIPVPIVSELQSGKWGAKIVMREDRSVRLSIQSSPKCIVGKFRMYVAVWTPYGVLRTSRNPETDTYILFNPWCEDDAVYLDNEKEREEYVLNDIGVIFYGEVNDIKTRSWSYGQFEDGILDTCLYVMDRAQMDLSGRGNPIKVSRVGSAMVNAKDDEGVLVGSWDNIYAYGVPPSAWTGSVDILLEYRSSENPVRYGQCWVFAGVFNTFLRCLGIPARIVTNYFSAHDNDANLQMDIFLEEDGNVNSKLTKDSVWNYHCWNEAWMTRPDLPVGFGGWQAVDSTPQENSDGMYRCGPASVQAIKHGHVCFQFDAPFVFAEVNSDLIYITAKKDGTHVVENVDATHIGKLIVTKQIGGDGMMDITDTYKFQEGQEEERLALETALMYGAKKPLNTEGVMKSRSNVDMDFEVENAVLGKDFKLSITFRNNSHNRYTITAYLSANITFYTGVPKAEFKKETFDVTLEPLSFKKEAVLIQAGEYMGQLLEQASLHFFVTARINETRDVLAKQKSTVLTIPEIIIKVRGTQVVGSDMTVTVQFTNPLKETLRNVWVHLDGPGVTRPMKKMFREIRPNSTVQWEEVCRPWVSGHRKLIASMSSDSLRHVYGELDVQIQRRPSM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
91
Chicken
82
Bovine
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00488 As Substrate

Site PTM Type Enzyme
S2 Acetylation
Y84 Phosphorylation
Y104 Phosphorylation
Y109 Phosphorylation
S124 Phosphorylation
Y161 Phosphorylation
Y241 Phosphorylation
K584 Acetylation
K585 Acetylation
N614 N-Glycosylation
T640 Phosphorylation
S645 Phosphorylation
T648 Phosphorylation
T650 Phosphorylation
K678 Acetylation
S688 Phosphorylation
T689 Phosphorylation
S711 Phosphorylation
S731 Phosphorylation

Research Backgrounds

Function:

Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.

PTMs:

The activation peptide is released by thrombin.

Subcellular Location:

Cytoplasm. Secreted.
Note: Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Tetramer of two A chains (F13A1) and two B (F13B) chains.

Family&Domains:

Belongs to the transglutaminase superfamily. Transglutaminase family.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

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