NUR77 Antibody - #DF7850

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Product: NUR77 Antibody
Catalog: DF7850
Description: Rabbit polyclonal antibody to NUR77
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 64 kDa; 64kD(Calculated).
Uniprot: P22736
RRID: AB_2841286

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(85%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(85%)
Clonality:
Polyclonal
Specificity:
NUR77 Antibody detects endogenous levels of total NUR77.
RRID:
AB_2841286
Cite Format: Affinity Biosciences Cat# DF7850, RRID:AB_2841286.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Early response protein NAK1; GFRP 1; GFRP; GFRP1; Growth factor inducible nuclear protein N10; Growth Factor Inducible Nuclear Protein NP10; Growth Factor Response Protein 1; Hbr1; HMR; Hormone Receptor; MGC9485; N10; N10 nuclear protein; NAK 1; NAK1; Nerve growth factor IB nuclear receptor variant 1; NGFIB; NP 10; NP10; NR4A1; NR4A1_HUMAN; Nuclear hormone receptor NUR/77; Nuclear Hormone Receptor TR3; Nuclear receptor subfamily 4 group A member 1; NUR77; NUR77, mouse, homolog of; Orphan nuclear receptor HMR; Orphan nuclear receptor NR4A1; Orphan nuclear receptor TR3; Orphan receptor tr3; Receptor NGFIB; ST 59; ST-59; ST59; Steroid receptor TR3; Testicular receptor 3; TR 3; TR3; TR3 orphan receptor;

Immunogens

Immunogen:

A synthesized peptide derived from human NUR77, corresponding to a region within the internal amino acids.

Uniprot:

P22736(NR4A1_HUMAN) >>Visit HPA database.

Gene(ID):
NR4A1(3164)
Expression:
P22736 NR4A1_HUMAN:

Fetal muscle and adult liver, brain and thyroid.

Sequence:
MPCIQAQYGTPAPSPGPRDHLASDPLTPEFIKPTMDLASPEAAPAAPTALPSFSTFMDGYTGEFDTFLYQLPGTVQPCSSASSSASSTSSSSATSPASASFKFEDFQVYGCYPGPLSGPVDEALSSSGSDYYGSPCSAPSPSTPSFQPPQLSPWDGSFGHFSPSQTYEGLRAWTEQLPKASGPPQPPAFFSFSPPTGPSPSLAQSPLKLFPSQATHQLGEGESYSMPTAFPGLAPTSPHLEGSGILDTPVTSTKARSGAPGGSEGRCAVCGDNASCQHYGVRTCEGCKGFFKRTVQKNAKYICLANKDCPVDKRRRNRCQFCRFQKCLAVGMVKEVVRTDSLKGRRGRLPSKPKQPPDASPANLLTSLVRAHLDSGPSTAKLDYSKFQELVLPHFGKEDAGDVQQFYDLLSGSLEVIRKWAEKIPGFAELSPADQDLLLESAFLELFILRLAYRSKPGEGKLIFCSGLVLHRLQCARGFGDWIDSILAFSRSLHSLLVDVPAFACLSALVLITDRHGLQEPRRVEELQNRIASCLKEHVAAVAGEPQPASCLSRLLGKLPELRTLCTQGLQRIFYLKLEDLVPPPPIIDKIFMDTLPF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Bovine
92
Sheep
92
Rabbit
92
Pig
85
Dog
85
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Plays a role in the vascular response to injury (By similarity).

PTMs:

Phosphorylated at Ser-351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli.

Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1.

Subcellular Location:

Nucleus. Cytoplasm. Mitochondrion.
Note: Nuclear export to the cytoplasm is XPO1-mediated and positively regulated by IFI27 (PubMed:22427340). Translocation to the mitochondrion upon interaction with RXRA and upon the presence of 9-cis retinoic acid (PubMed:17761950).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Fetal muscle and adult liver, brain and thyroid.

Family&Domains:

Belongs to the nuclear hormone receptor family. NR4 subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

References

1). Natural product mogrol attenuates bleomycin-induced pulmonary fibrosis development through promoting AMPK activation. Journal of Functional Foods, 2021 [IF=3.8]

Application: WB    Species: Mouse    Sample: lung tissues

Fig. 5. . Mogrol attenuated idative oxidative stress and TGF-β1/Smad but upregulated AMPK/Sirt1 signaling pathways in pulmonary fibrosis mice. Pulmonary fibrosis was induced by an intratracheal instillation of bleomycin (BLM). Mice received a gavage of vehicle (0.5% CMC-Na) or mogrol (MG, 1, 5 or 10 mg/kg/d) or nintedanib (NDN, positive drug, 40 mg/kg/d) 7 days after and mice were euthanized on day 22 after bleomycin injection. (a) The protein expression of NOX4 in lung tissues were determined by western blotting. (b) The mRNA levels of NOX4 in lung tissues were determined by PCR analysis. (c and d) Representative images of immunohistochemical analysis of NOX4 expression in the lung section (brown). The protein expression of TGF-β1 (e), Sirt1 (i) and NR4A1 (j) were determined by western blotting. (f) The mRNA levels of TGF-β1 in lung tissues were determined by PCR analysis. The phosphorylation levels of Smad2/3 (g) and AMPK (h) in lung tissues were determined by western blotting. Data are presented as mean ± SD (n = 9).

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