TRIB3 Antibody - #DF7844

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Product: TRIB3 Antibody
Catalog: DF7844
Description: Rabbit polyclonal antibody to TRIB3
Application: WB IHC
Reactivity: Human
Prediction: Pig, Bovine, Sheep
Mol.Wt.: 40 kDa; 40kD(Calculated).
Uniprot: Q96RU7
RRID: AB_2841282

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(80%), Bovine(93%), Sheep(93%)
Clonality:
Polyclonal
Specificity:
TRIB3 Antibody detects endogenous levels of total TRIB3.
RRID:
AB_2841282
Cite Format: Affinity Biosciences Cat# DF7844, RRID:AB_2841282.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C20orf97; Neuronal cell death inducible putative kinase; Neuronal cell death-inducible putative kinase; NIPK; p65 interacting inhibitor of NF-kappaB; p65-interacting inhibitor of NF-kappa-B; SINK; SKIP 3; SKIP3; TRB 3; TRB-3; TRB3; TRIB 3; Trib3; TRIB3_HUMAN; Tribbles homolog 3; Tribbles pseudokinase 3; Tribbles3;

Immunogens

Immunogen:
Uniprot:

Q96RU7(TRIB3_HUMAN) >>Visit HPA database.

Gene(ID):
TRIB3(57761)
Expression:
Q96RU7 TRIB3_HUMAN:

Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.

Sequence:
MRATPLAAPAGSLSRKKRLELDDNLDTERPVQKRARSGPQPRLPPCLLPLSPPTAPDRATAVATASRLGPYVLLEPEEGGRAYQALHCPTGTEYTCKVYPVQEALAVLEPYARLPPHKHVARPTEVLAGTQLLYAFFTRTHGDMHSLVRSRHRIPEPEAAVLFRQMATALAHCHQHGLVLRDLKLCRFVFADRERKKLVLENLEDSCVLTGPDDSLWDKHACPAYVGPEILSSRASYSGKAADVWSLGVALFTMLAGHYPFQDSEPVLLFGKIRRGAYALPAGLSAPARCLVRCLLRREPAERLTATGILLHPWLRQDPMPLAPTRSHLWEAAQVVPDGLGLDEAREEEGDREVVLYG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
93
Sheep
93
Pig
80
Rabbit
69
Dog
64
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96RU7 As Substrate

Site PTM Type Enzyme
T4 Phosphorylation
S12 Phosphorylation
S14 Phosphorylation
K17 Ubiquitination
K33 Ubiquitination
S51 Phosphorylation
T64 Phosphorylation
K97 Ubiquitination
K197 Ubiquitination
S215 Phosphorylation
K219 Ubiquitination

Research Backgrounds

Function:

Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. Can inhibit APOBEC3A editing of nuclear DNA.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.

Subunit Structure:

Interacts with AKT1, AKT2, ATF4, MAP2K1 and MAP2K7. Interacts with DDIT3/CHOP and inhibits its interaction with EP300/P300. Interacts (via N-terminus) with APOBEC3A. Interacts with APOBEC3C.

Family&Domains:

The protein kinase domain is predicted to be catalytically inactive.

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

References

1). Developing an advanced diagnostic model for hepatocellular carcinoma through multi-omics integration leveraging diverse cell-death patterns. Frontiers in immunology, 2024 (PubMed: 39044829) [IF=7.3]

Application: IHC    Species: Human    Sample: HCC cell

Figure 7 The expression pattern of seven key genes in the scRNA-seq and stRNA-seq data. (A-G) The violin plots on the left side illustrate the expression levels of such seven key genes in the HCC cell subsets of two independent datasets of GSE149614 and GSE151530, while the violin plots on the right side present their corresponding expression patterns in the normal hepatocytes, HCC cells, and proliferative HCC cells in the external validation scRNA-seq dataset. Their spatial expression distribution in normal liver, ICB responders, and ICB non-responders is shown in the right panel. (H) IHC analysis shows upregulated TRIB3 and NQO1 expression in tissue samples of HCC patients relative to the normal controls. * P
2). Identification and preliminary analysis of hub genes associated with bladder cancer progression by comprehensive bioinformatics analysis. Scientific reports, 2024 (PubMed: 38307969) [IF=4.6]
3). Comparing Tribbles Homolog 3 (TRIB3) Protein Expression Levels with Clinicopathological Characteristics and Survival Among Neuroblastoma Patients. Clinical Medicine Insights. Oncology, 2023 (PubMed: 37744426) [IF=2.2]

Application: IHC    Species: Human    Sample:

Figure 1. Immunohistochemical staining of TRIB3 in NB patient specimens. TRIB3 positive staining showed cytoplasmic staining. (A) TRIB3 positive differentiating NB. The red arrow indicated the TRIB3 positive cell, which was brown in color. (B) TRIB3 negative differentiating NB. The black arrow indicated TRIB3 negative cell, which was blue in color. (×100 magnification, Bar: 20 μm). NB indicates neuroblastoma; TRIB3, tribbles homolog 3.

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