NCAM1 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/5

Western blot analysis of NCAM1 using RAW264.

DF7832 at 1/100 staining Human kidney cancer by IHC-P.

DF7832 at 1/100 staining Human ovarian cancer by IHC-P.

DF7832 at 1/100 staining Mouse liver tissue by IHC-P.

DF7832 staining Hela cells by IF/ICC.

Product:	NCAM1 Antibody
Catalog:	DF7832
Description:	Rabbit polyclonal antibody to NCAM1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Bovine, Sheep, Rabbit, Chicken
Mol.Wt.:	180 kDa; 95kD(Calculated).
Uniprot:	P13591
RRID:	AB_2841273

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Bovine(100%), Sheep(100%), Rabbit(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

NCAM1 Antibody detects endogenous levels of total NCAM1.

RRID:

AB_2841273
Cite Format: Affinity Biosciences Cat# DF7832, RRID:AB_2841273.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

antigen MSK39 identified by monoclonal antibody 5.1H11; antigen recognized by monoclonal antibody 5.1H11; CD56; cell adhesion molecule, neural, 1; MSK 39; MSK39; N-CAM-1; NCAM 1; NCAM; NCAM C; NCAM-1; NCAM1; NCAM1_HUMAN; NCAMC; Neural cell adhesion molecule 1; Neural cell adhesion molecule NCAM; OTTHUMP00000235666;

Immunogens

Immunogen:

Uniprot:

P13591(NCAM1_HUMAN) >>Visit HPA database.

Gene(ID):

NCAM1(4684)

Sequence:

P13591 NCAM1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLQTKDLIWTLFFLGTAVSLQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLTPNQQRISVVWNDDSSSTLTIYNANIDDAGIYKCVVTGEDGSESEATVNVKIFQKLMFKNAPTPQEFREGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTIQARQNIVNATANLGQSVTLVCDAEGFPEPTMSWTKDGEQIEQEEDDEKYIFSDDSSQLTIKKVDKNDEAEYICIAENKAGEQDATIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDQVEPYSSTAQVQFDEPEATGGVPILKYKAEWRAVGEEVWHSKWYDAKEASMEGIVTIVGLKPETTYAVRLAALNGKGLGEISAASEFKTQPVQGEPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVRYRALSSEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTSGLSTGAIVGILIVIFVLLLVVVDITCYFLNKCGLFMCIAVNLCGKAGPGAKGKDMEEGKAAFSKDESKEPIVEVRTEEERTPNHDGGKHTEPNETTPLTEPEKGPVEAKPECQETETKPAPAEVKTVPNDATQTKENESKA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Chicken

100

Rabbit

100

Xenopus

Pig

Horse

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13591 As Substrate

P13591

Site	PTM Type	Source
T125	O-Glycosylation	Uniprot
R130	Methylation	Uniprot
T328	Phosphorylation	Uniprot
S398	Phosphorylation	Uniprot
N459	N-Glycosylation	Uniprot
S566	Phosphorylation	Uniprot
T605	Phosphorylation	Uniprot
S613	Phosphorylation	Uniprot
Y744	Phosphorylation	Uniprot
K776	Ubiquitination	Uniprot
S780	Phosphorylation	Uniprot
S784	Phosphorylation	Uniprot
K785	Ubiquitination	Uniprot
C829	S-Nitrosylation	Uniprot
T849	Phosphorylation	Uniprot
K852	Ubiquitination	Uniprot

Research Backgrounds

P13591_NCAM1_HUMAN

Function:

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

(Microbial infection) Acts as a receptor for rabies virus.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Cell membrane>Single-pass type I membrane protein.

Cell membrane>Lipid-anchor.

Cell membrane>Lipid-anchor.

Secreted.

Secreted.

Subunit Structure:

(Microbial infection) Interacts with rabies virus glycoprotein.

Interacts with MDK.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Neurodegenerative diseases > Prion diseases.

Restrictive clause

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For Research Use Only.
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NCAM1 Antibody - #DF7832