B-Myb Antibody - #DF7817

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Product: B-Myb Antibody
Catalog: DF7817
Description: Rabbit polyclonal antibody to B-Myb
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 79kDa; 79kD(Calculated).
Uniprot: P10244
RRID: AB_2841266

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
B-Myb Antibody detects endogenous levels of total B-Myb.
RRID:
AB_2841266
Cite Format: Affinity Biosciences Cat# DF7817, RRID:AB_2841266.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B-Myb; BMyB; MGC15600; MYB L2; Myb related protein B; Myb-like protein 2; Myb-related protein B; MybB; MYBB_HUMAN; MYBL 2; Mybl2; v myb avian myeloblastosis viral oncogene homolog like 2; v myb myeloblastosis viral oncogene homolog (avian) like 2; v myb myeloblastosis viral oncogene homolog like 2;

Immunogens

Immunogen:
Uniprot:

P10244(MYBB_HUMAN) >>Visit HPA database.

Gene(ID):
MYBL2(4605)
Sequence:
MSRRTRCEDLDELHYQDTDSDVPEQRDSKCKVKWTHEEDEQLRALVRQFGQQDWKFLASHFPNRTDQQCQYRWLRVLNPDLVKGPWTKEEDQKVIELVKKYGTKQWTLIAKHLKGRLGKQCRERWHNHLNPEVKKSCWTEEEDRIICEAHKVLGNRWAEIAKMLPGRTDNAVKNHWNSTIKRKVDTGGFLSESKDCKPPVYLLLELEDKDGLQSAQPTEGQGSLLTNWPSVPPTIKEEENSEEELAAATTSKEQEPIGTDLDAVRTPEPLEEFPKREDQEGSPPETSLPYKWVVEAANLLIPAVGSSLSEALDLIESDPDAWCDLSKFDLPEEPSAEDSINNSLVQLQASHQQQVLPPRQPSALVPSVTEYRLDGHTISDLSRSSRGELIPISPSTEVGGSGIGTPPSVLKRQRKRRVALSPVTENSTSLSFLDSCNSLTPKSTPVKTLPFSPSQFLNFWNKQDTLELESPSLTSTPVCSQKVVVTTPLHRDKTPLHQKHAAFVTPDQKYSMDNTPHTPTPFKNALEKYGPLKPLPQTPHLEEDLKEVLRSEAGIELIIEDDIRPEKQKRKPGLRRSPIKKVRKSLALDIVDEDVKLMMSTLPKSLSLPTTAPSNSSSLTLSGIKEDNSLLNQGFLQAKPEKAAVAQKPRSHFTTPAPMSSAWKTVACGGTRDQLFMQEKARQLLGRLKPSHTSRTLILS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
80
Chicken
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10244 As Substrate

Site PTM Type Enzyme
Y15 Phosphorylation
T18 Phosphorylation P68400 (CSNK2A1)
S20 Phosphorylation
S28 Phosphorylation
K33 Ubiquitination
K93 Sumoylation
K104 Sumoylation
K134 Sumoylation
K134 Ubiquitination
K151 Ubiquitination
K162 Ubiquitination
K173 Ubiquitination
S178 Phosphorylation
K181 Ubiquitination
K183 Ubiquitination
K197 Sumoylation
T234 Phosphorylation
K236 Sumoylation
S241 Phosphorylation
T266 Phosphorylation
S282 Phosphorylation
T286 Phosphorylation
S287 Phosphorylation
S362 Phosphorylation
S393 Phosphorylation
T396 Phosphorylation
T405 Phosphorylation
K411 Ubiquitination
S421 Phosphorylation
T440 Phosphorylation
S443 Phosphorylation
T444 Phosphorylation P24941 (CDK2)
K447 Ubiquitination
S452 Phosphorylation
S454 Phosphorylation
T465 Phosphorylation
S470 Phosphorylation
S472 Phosphorylation
S475 Phosphorylation
T476 Phosphorylation
T486 Phosphorylation
T487 Phosphorylation P24941 (CDK2)
T494 Phosphorylation P24941 (CDK2)
K499 Ubiquitination
T505 Phosphorylation
K509 Sumoylation
K509 Ubiquitination
T515 Phosphorylation
T518 Phosphorylation
T520 Phosphorylation P24941 (CDK2)
K523 Ubiquitination
K528 Ubiquitination
K533 Acetylation
K533 Ubiquitination
T538 Phosphorylation
K546 Sumoylation
K546 Ubiquitination
S577 Phosphorylation P24941 (CDK2)
K584 Ubiquitination
S585 Phosphorylation
K596 Sumoylation
K625 Ubiquitination
S629 Phosphorylation
K639 Sumoylation
K639 Ubiquitination
K648 Ubiquitination
T693 Phosphorylation
R695 Methylation

Research Backgrounds

Function:

Transcription factor involved in the regulation of cell survival, proliferation, and differentiation. Transactivates the expression of the CLU gene.

PTMs:

Phosphorylated by cyclin A/CDK2 during S-phase. Phosphorylation at Thr-520 is probably involved in transcriptional activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL22.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

References

1). LncRNA-MALAT1 Regulates Cancer Glucose Metabolism in Prostate Cancer via MYBL2/mTOR Axis. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35557985)

Application: WB    Species: Mouse    Sample: Tumor tissues

Figure 2 MALAT1 regulates downstream mRNA MYBL2 and confer PCa progression. (a) QRT-PCR verified the MYBL2 levels in MALAT1 knockdown PC-3 cell line (left panel) and C4-2 cell line (right panel). (b) Western blot examines MYBL2 level in MALAT1 knockdown PC-3 and C4-2 cell lines (left panel), and quantification (right panel) of western blot result. (c) QRT-PCR displayed the knockdown efficiency in PC-3 and C4-2 by treating with MYBL2 siRNA. (d) Western blot tests MYBL2 level after treating PC-3 and C4-2 cell line with siMYBL2 (left panel) and quantification (right panel) of western blot result. (e) QRT-PCR examines the MALAT1 level in knockdown MYBL2 PC-3 cell line (left panel) and in C4-2 (right panel). (f) Representative images of EdU assay formed by the vector control PC-3 cell line and MALAT1 or MYBL2 knockdown cell lines. Scale bars: 50 μm. (h) Representative images of colonies formed by vector control PC-3 cell line and MALAT1 or MYBL2 knockdown cell lines. (g and i) Quantification of EdU assay (left panel) and colonies (right panel). All samples were normalized to GAPDH mRNA and protein levels. The error bars indicate SD (n = 3). Student t test was used. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Application: IHC    Species: Mouse    Sample: Tumor tissues

Figure 5 MALAT1/MYBL2/mTOR axis regulates PCa progression and glucose metabolism in mouse model. (a) The tumorigenic ability of nude mouse model (n = 6) between injected knockdown MALAT1 PC-3 cell siMALAT1 group and vector control NC group. (b) Final tumor weight between siMALAT1 group and NC group. (c) Tumor volume growth between two groups. (d) Representative images of hematoxylin-eosin staining between two groups. Scale bars: 100 μm. (e–i) Representative images of immunohistochemistry of Ki67 (e), MYBL2 (f), mTOR (g), p-mTOR (h), and PKM2 (i) between siMALAT1 group and NC group. (j) Quantification of IHC results. Scale bars: 100 μm. The error bars indicate SD (n = 6). Student t test was used. ∗P < 0.05, ∗∗P < 0.01.

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