FYB Antibody - #DF7813

(2)  
Product: FYB Antibody
Catalog: DF7813
Description: Rabbit polyclonal antibody to FYB
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 110 kDa; 85kD(Calculated).
Uniprot: O15117
RRID: AB_2841264

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(83%), Horse(83%), Sheep(83%), Dog(82%)
Clonality:
Polyclonal
Specificity:
FYB Antibody detects endogenous levels of total FYB.
RRID:
AB_2841264
Cite Format: Affinity Biosciences Cat# DF7813, RRID:AB_2841264.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADAP; Adhesion and degranulation promoting adaptor protein; FYB 120/130; Fyb; FYB-120/130; FYB_HUMAN; FYN binding protein; FYN T binding protein; FYN-binding protein; FYN-T-binding protein; p120/p130; PRO0823; SLAP 130; SLAP-130; SLAP130; SLP 76 associated phosphoprotein; SLP-76-associated phosphoprotein; SLP76 associated phosphoprotein;

Immunogens

Immunogen:
Uniprot:

O15117(FYB1_HUMAN) >>Visit HPA database.

Gene(ID):
FYB1(2533)
Expression:
O15117 FYB1_HUMAN:

Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues.

Sequence:
MAKYNTGGNPTEDVSVNSRPFRVTGPNSSSGIQARKNLFNNQGNASPPAGPSNVPKFGSPKPPVAVKPSSEEKPDKEPKPPFLKPTGAGQRFGTPASLTTRDPEAKVGFLKPVGPKPINLPKEDSKPTFPWPPGNKPSLHSVNQDHDLKPLGPKSGPTPPTSENEQKQAFPKLTGVKGKFMSASQDLEPKPLFPKPAFGQKPPLSTENSHEDESPMKNVSSSKGSPAPLGVRSKSGPLKPAREDSENKDHAGEISSLPFPGVVLKPAASRGGPGLSKNGEEKKEDRKIDAAKNTFQSKINQEELASGTPPARFPKAPSKLTVGGPWGQSQEKEKGDKNSATPKQKPLPPLFTLGPPPPKPNRPPNVDLTKFHKTSSGNSTSKGQTSYSTTSLPPPPPSHPASQPPLPASHPSQPPVPSLPPRNIKPPFDLKSPVNEDNQDGVTHSDGAGNLDEEQDSEGETYEDIEASKEREKKREKEEKKRLELEKKEQKEKEKKEQEIKKKFKLTGPIQVIHLAKACCDVKGGKNELSFKQGEQIEIIRITDNPEGKWLGRTARGSYGYIKTTAVEIDYDSLKLKKDSLGAPSRPIEDDQEVYDDVAEQDDISSHSQSGSGGIFPPPPDDDIYDGIEEEDADDGFPAPPKQLDMGDEVYDDVDTSDFPVSSAEMSQGTNVGKAKTEEKDLKKLKKQEKEEKDFRKKFKYDGEIRVLYSTKVTTSITSKKWGTRDLQVKPGESLEVIQTTDDTKVLCRNEEGKYGYVLRSYLADNDGEIYDDIADGCIYDND

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
83
Bovine
83
Sheep
83
Dog
82
Rabbit
70
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15117 As Substrate

Site PTM Type Enzyme
K3 Acetylation
Y4 Phosphorylation
S30 Phosphorylation
S46 Phosphorylation
T94 Phosphorylation
K111 Acetylation
K111 Ubiquitination
K149 Acetylation
T158 Phosphorylation
T161 Phosphorylation
K167 Ubiquitination
K179 Acetylation
S184 Phosphorylation
S209 Phosphorylation
S214 Phosphorylation
S222 Phosphorylation
K223 Ubiquitination
S225 Phosphorylation
S233 Phosphorylation
S235 Phosphorylation
S245 Phosphorylation
K265 Acetylation
K287 Ubiquitination
K292 Ubiquitination
K298 Ubiquitination
S306 Phosphorylation
T308 Phosphorylation
S318 Phosphorylation
K319 Ubiquitination
S329 Phosphorylation
K334 Acetylation
K373 Ubiquitination
T374 Phosphorylation
T385 Phosphorylation
S386 Phosphorylation
Y387 Phosphorylation
K425 Acetylation
S432 Phosphorylation
S457 Phosphorylation
T461 Phosphorylation
Y462 Phosphorylation
K496 Ubiquitination
K503 Ubiquitination
T507 Phosphorylation
K549 Ubiquitination
S558 Phosphorylation
Y559 Phosphorylation P06241 (FYN)
Y561 Phosphorylation
Y571 Phosphorylation P06241 (FYN)
S573 Phosphorylation
K575 Ubiquitination
Y595 Phosphorylation P06241 (FYN)
Y625 Phosphorylation P06241 (FYN)
Y651 Phosphorylation P06241 (FYN)
Y701 Phosphorylation
Y709 Phosphorylation
T711 Phosphorylation
K730 Ubiquitination
S734 Phosphorylation
K745 Ubiquitination
K754 Ubiquitination
Y755 Phosphorylation P06241 (FYN)
Y757 Phosphorylation P06241 (FYN)
Y762 Phosphorylation
Y771 Phosphorylation P06241 (FYN)
Y780 Phosphorylation P06241 (FYN)

Research Backgrounds

Function:

Acts as an adapter protein of the FYN and LCP2 signaling cascades in T-cells (By similarity). May play a role in linking T-cell signaling to remodeling of the actin cytoskeleton. Modulates the expression of IL2 (By similarity). Involved in platelet activation (By similarity). Prevents the degradation of SKAP1 and SKAP2. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells (By similarity).

PTMs:

T-cell receptor ligation leads to increased tyrosine phosphorylation.

Subcellular Location:

Cytoplasm. Nucleus. Cell junction.
Note: Colocalizes with TMEM47 at cell-cell contacts in podocytes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in hematopoietic tissues such as myeloid and T-cells, spleen and thymus. Not expressed in B-cells, nor in non-lymphoid tissues.

Subunit Structure:

Part of a complex consisting of SKAP2, FYB1 and PTPNS1 (By similarity). Part of a complex consisting of SKAP2, FYB1 and LILRB3 (By similarity). Part of a complex consisting of SKAP1, FYB1 and CLNK (By similarity). Interacts with CLNK (via its SH2 domain); this interaction allows SKAP1 and FYB1 to recruit FYN to the complex, thus promoting the phosphorylation of CLNK by FYN (By similarity). Interacts with FYN. Interacts with LCP2. Interacts with SKAP1. Interacts with SKAP2. Interacts with FASLG. Interacts with EVL. Interacts with TMEM47 (By similarity). Interacts with LCK.

Research Fields

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

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