HNRNPUL1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from C6 cells, using HNRNPUL1 Antibody.

Product:	HNRNPUL1 Antibody
Catalog:	DF7810
Description:	Rabbit polyclonal antibody to HNRNPUL1
Application:	WB IF/ICC
Reactivity:	Human, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.:	96 kDa; 96kD(Calculated).
Uniprot:	Q9BUJ2
RRID:	AB_2841262

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(100%), Bovine(92%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

HNRNPUL1 Antibody detects endogenous levels of total HNRNPUL1.

RRID:

AB_2841262
Cite Format: Affinity Biosciences Cat# DF7810, RRID:AB_2841262.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Adenovirus early region 1B associated protein 5; Adenovirus early region 1B-associated protein 5; E130317O14Rik; E1B 55 kDa associated protein 5; E1B AP5; E1B-55 kDa-associated protein 5; E1B-AP5; E1BAP5; fb53d10; fk45c03; FLJ12944; Heterogeneous nuclear ribonucleoprotein U-like protein 1; HNRL1_HUMAN; Hnrnpul1; HNRPUL1; MGC36621; MGC78330; wu:fb53d10; wu:fk45c03; zgc:85971;

Immunogens

Immunogen:

Uniprot:

Q9BUJ2(HNRL1_HUMAN) >>Visit HPA database.

Gene(ID):

HNRNPUL1(11100)

Sequence:

Q9BUJ2 HNRL1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDVRRLKVNELREELQRRGLDTRGLKAELAERLQAALEAEEPDDERELDADDEPGRPGHINEEVETEGGSELEGTAQPPPPGLQPHAEPGGYSGPDGHYAMDNITRQNQFYDTQVIKQENESGYERRPLEMEQQQAYRPEMKTEMKQGAPTSFLPPEASQLKPDRQQFQSRKRPYEENRGRGYFEHREDRRGRSPQPPAEEDEDDFDDTLVAIDTYNCDLHFKVARDRSSGYPLTIEGFAYLWSGARASYGVRRGRVCFEMKINEEISVKHLPSTEPDPHVVRIGWSLDSCSTQLGEEPFSYGYGGTGKKSTNSRFENYGDKFAENDVIGCFADFECGNDVELSFTKNGKWMGIAFRIQKEALGGQALYPHVLVKNCAVEFNFGQRAEPYCSVLPGFTFIQHLPLSERIRGTVGPKSKAECEILMMVGLPAAGKTTWAIKHAASNPSKKYNILGTNAIMDKMRVMGLRRQRNYAGRWDVLIQQATQCLNRLIQIAARKKRNYILDQTNVYGSAQRRKMRPFEGFQRKAIVICPTDEDLKDRTIKRTDEEGKDVPDHAVLEMKANFTLPDVGDFLDEVLFIELQREEADKLVRQYNEEGRKAGPPPEKRFDNRGGGGFRGRGGGGGFQRYENRGPPGGNRGGFQNRGGGSGGGGNYRGGFNRSGGGGYSQNRWGNNNRDNNNSNNRGSYNRAPQQQPPPQQPPPPQPPPQQPPPPPSYSPARNPPGASTYNKNSNIPGSSANTSTPTVSSYSPPQPSYSQPPYNQGGYSQGYTAPPPPPPPPPAYNYGSYGGYNPAPYTPPPPPTAQTYPQPSYNQYQQYAQQWNQYYQNQGQWPPYYGNYDYGSYSGNTQGGTSTQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Rabbit

100

Bovine

Xenopus

Zebrafish

Sheep

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BUJ2 As Substrate

Q9BUJ2

Site	PTM Type	Enzyme	Source
R23	Methylation		Uniprot
K26	Ubiquitination		Uniprot
Y111	Phosphorylation		Uniprot
K117	Sumoylation		Uniprot
K117	Ubiquitination		Uniprot
Y124	Phosphorylation		Uniprot
Y137	Phosphorylation		Uniprot
K142	Sumoylation		Uniprot
K142	Ubiquitination		Uniprot
K146	Sumoylation		Uniprot
K146	Ubiquitination		Uniprot
T151	Phosphorylation		Uniprot
S152	Phosphorylation		Uniprot
S159	Phosphorylation		Uniprot
K162	Acetylation		Uniprot
K162	Ubiquitination		Uniprot
K172	Methylation		Uniprot
K172	Ubiquitination		Uniprot
Y175	Phosphorylation		Uniprot
R179	Methylation		Uniprot
R181	Methylation		Uniprot
Y183	Phosphorylation		Uniprot
S194	Phosphorylation		Uniprot
T209	Phosphorylation		Uniprot
T215	Phosphorylation		Uniprot
K270	Acetylation		Uniprot
K270	Ubiquitination		Uniprot
K309	Ubiquitination		Uniprot
K310	Ubiquitination		Uniprot
K350	Ubiquitination		Uniprot
C377	S-Nitrosylation		Uniprot
K418	Ubiquitination		Uniprot
K440	Acetylation		Uniprot
K440	Methylation		Uniprot
K440	Sumoylation		Uniprot
K440	Ubiquitination		Uniprot
S444	Phosphorylation		Uniprot
K449	Sumoylation		Uniprot
K449	Ubiquitination		Uniprot
Y450	Phosphorylation		Uniprot
T455	Phosphorylation		Uniprot
K461	Ubiquitination		Uniprot
C487	S-Nitrosylation		Uniprot
Y510	Phosphorylation		Uniprot
S512	Phosphorylation		Uniprot
K517	Ubiquitination		Uniprot
K527	Sumoylation		Uniprot
K527	Ubiquitination		Uniprot
C532	S-Nitrosylation		Uniprot
K539	Sumoylation		Uniprot
K539	Ubiquitination		Uniprot
R584	Methylation		Uniprot
R592	Methylation		Uniprot
K600	Ubiquitination		Uniprot
R612	Methylation		Uniprot
R618	Methylation		Uniprot
R620	Methylation		Uniprot
R628	Methylation		Uniprot
R632	Methylation		Uniprot
R639	Methylation		Uniprot
R645	Methylation		Uniprot
S649	Phosphorylation		Uniprot
R656	Methylation		Uniprot
R661	Methylation		Uniprot
S668	Phosphorylation		Uniprot
R671	Methylation		Uniprot
R685	Methylation		Uniprot
S687	Phosphorylation		Uniprot
R690	Methylation		Uniprot
S716	Phosphorylation	P24941 (CDK2)	Uniprot
Y717	Phosphorylation		Uniprot
S718	Phosphorylation	P24941 (CDK2)	Uniprot

Research Backgrounds

Q9BUJ2_HNRL1_HUMAN

Function:

Acts as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Plays also a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro.

PTMs:

Methylated.

Subcellular Location:

Nucleus.

Subunit Structure:

Interacts with the adenovirus type 5 (Ad5) E1B-55 kDa, BRD7, PRMT2, TP53 and NXF1. Associates with histones and BRD7.

Family&Domains:

The RGG-box domain is methylated.

Research Fields

· Human Diseases > Infectious diseases: Viral > Influenza A.

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HNRNPUL1 Antibody - #DF7810