Calpain 2 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/3

Western blot analysis of CAPN2 using RAW264.

Figure 1 Calpain 1 and 2 are markedly increased in the renal biopsy of AKI patients.

Product:	Calpain 2 Antibody
Catalog:	DF7807
Description:	Rabbit polyclonal antibody to Calpain 2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Chicken
Mol.Wt.:	80kDa; 80kD(Calculated).
Uniprot:	P17655
RRID:	AB_2841260

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Sheep(100%), Chicken(86%)

Clonality:

Polyclonal

Specificity:

Calpain 2 Antibody detects endogenous levels of total Calpain 2.

RRID:

AB_2841260
Cite Format: Affinity Biosciences Cat# DF7807, RRID:AB_2841260.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Calcium activated neutral proteinase 2; Calcium activated neutral proteinase; Calcium-activated neutral protease 2, catalytic subunit; Calcium-activated neutral proteinase 2; CALP80; Calpain 2 (m/II) large subunit; Calpain 2 catalytic subunit; Calpain 2 large catalytic subunit; Calpain 2 large subunit; Calpain 2, large [catalytic] subunit; Calpain large polypeptide L2; Calpain M type; Calpain M-type; Calpain-2 catalytic subunit; Calpain-2 large subunit; Calpain2; CAN2_HUMAN; CANP 2; CANP L2; CANP2; CANPL 2; CANPL2; CANPml; Capa2; CAPN 2; CAPN2; FLJ39928; M calpain; M calpin; M type; M-calpain; mCANP; Millimolar calpain; Millimolar-calpain;

Immunogens

Immunogen:

Uniprot:

P17655(CAN2_HUMAN) >>Visit HPA database.

Gene(ID):

CAPN2(824)

Expression:

P17655 CAN2_HUMAN:

Ubiquitous.

Sequence:

P17655 CAN2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGIAAKLAKDREAAEGLGSHDRAIKYLNQDYEALRNECLEAGTLFQDPSFPAIPSALGFKELGPYSSKTRGIEWKRPTEICADPQFIIGGATRTDICQGALGDCWLLAAIASLTLNEEILARVVPLNQSFQENYAGIFHFQFWQYGEWVEVVVDDRLPTKDGELLFVHSAEGSEFWSALLEKAYAKINGCYEALSGGATTEGFEDFTGGIAEWYELKKPPPNLFKIIQKALQKGSLLGCSIDITSAADSEAITFQKLVKGHAYSVTGAEEVESNGSLQKLIRIRNPWGEVEWTGRWNDNCPSWNTIDPEERERLTRRHEDGEFWMSFSDFLRHYSRLEICNLTPDTLTSDTYKKWKLTKMDGNWRRGSTAGGCRNYPNTFWMNPQYLIKLEEEDEDEEDGESGCTFLVGLIQKHRRRQRKMGEDMHTIGFGIYEVPEELSGQTNIHLSKNFFLTNRARERSDTFINLREVLNRFKLPPGEYILVPSTFEPNKDGDFCIRVFSEKKADYQAVDDEIEANLEEFDISEDDIDDGFRRLFAQLAGEDAEISAFELQTILRRVLAKRQDIKSDGFSIETCKIMVDMLDSDGSGKLGLKEFYILWTKIQKYQKIYREIDVDRSGTMNSYEMRKALEEAGFKMPCQLHQVIVARFADDQLIIDFDNFVRCLVRLETLFKIFKQLDPENTGTIELDLISWLCFSVL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Chicken

Pig

Horse

Dog

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17655 As Substrate

P17655

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
K7	Acetylation		Uniprot
R23	Methylation		Uniprot
K26	Ubiquitination		Uniprot
Y32	Phosphorylation		Uniprot
S50	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
K61	Acetylation		Uniprot
K61	Ubiquitination		Uniprot
S68	Phosphorylation		Uniprot
K69	Ubiquitination		Uniprot
C82	S-Nitrosylation		Uniprot
K219	Ubiquitination		Uniprot
K230	Ubiquitination		Uniprot
K234	Ubiquitination		Uniprot
K257	Ubiquitination		Uniprot
K260	Ubiquitination		Uniprot
K280	Ubiquitination		Uniprot
Y353	Phosphorylation		Uniprot
K354	Acetylation		Uniprot
K355	Acetylation		Uniprot
K360	Ubiquitination		Uniprot
S369	Phosphorylation	P17612 (PRKACA)	Uniprot
T370	Phosphorylation	P17612 (PRKACA)	Uniprot
K390	Sumoylation		Uniprot
S403	Phosphorylation		Uniprot
K414	Ubiquitination		Uniprot
S462	Phosphorylation		Uniprot
T464	Phosphorylation		Uniprot
K476	Ubiquitination		Uniprot
K568	Ubiquitination		Uniprot
S569	Phosphorylation		Uniprot
C577	S-Nitrosylation		Uniprot
S586	Phosphorylation		Uniprot
S589	Phosphorylation		Uniprot
K591	Ubiquitination		Uniprot
Y611	Phosphorylation		Uniprot
T621	Phosphorylation		Uniprot
Y625	Phosphorylation		Uniprot
K629	Ubiquitination		Uniprot
K674	Ubiquitination		Uniprot

Research Backgrounds

P17655_CAN2_HUMAN

Function:

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves MYOC at 'Arg-226'. Proteolytically cleaves CPEB3 following neuronal stimulation which abolishes CPEB3 translational repressor activity, leading to translation of CPEB3 target mRNAs (By similarity).

Subcellular Location:

Cytoplasm. Cell membrane.
Note: Translocates to the plasma membrane upon Ca(2+) binding.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Forms a heterodimer with a small (regulatory) subunit (CAPNS1). Interacts with CPEB3; this leads to cleavage of CPEB3.

Family&Domains:

Belongs to the peptidase C2 family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

References

1). Pelargonic acid vanillylamide alleviates hepatic autophagy and ER stress in hepatic steatosis model. Food and Chemical Toxicology, 2023 (PubMed: 37611858) [IF=4.3]

2). Calpain Inhibitor Calpeptin Alleviates Ischemia/Reperfusion-Induced Acute Kidney Injury via Suppressing AIM2 Inflammasome and Upregulating Klotho Protein. Frontiers in Medicine, 2022 (PubMed: 35155498) [IF=3.9]

Application: IHC Species: Human Sample: kidney issues

Figure 1 Calpain 1 and 2 are markedly increased in the renal biopsy of AKI patients. Representative immunohistochemical micrographs of Calpain 1 (A) and Calpain 2 (C) protein expression in the renal biopsy of both AKI patients (n = 10) and normal control group (n = 6). Quantitative analysis of Calpain 1 (B) and Calpain 2 (D) expression in the human renal specimens. ×200, bar = 100 μm. Data were presented as mean ± SEM (n = 3). **p < 0.01; ***p < 0.001 vs. normal control group. AKI, acute kidney injury.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Calpain 2 Antibody - #DF7807