NODAL Antibody - #DF7791

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Product: NODAL Antibody
Catalog: DF7791
Description: Rabbit polyclonal antibody to NODAL
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 40 kDa, 30 kDa; 40kD(Calculated).
Uniprot: Q96S42
RRID: AB_2841248

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
NODAL Antibody detects endogenous levels of total NODAL.
RRID:
AB_2841248
Cite Format: Affinity Biosciences Cat# DF7791, RRID:AB_2841248.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HTX5; MGC138230; Nodal; Nodal Growth Differentiation Factor; nodal homolog (mouse); Nodal homolog; Nodal, Mouse, Homolog; NODAL_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q96S42(NODAL_HUMAN) >>Visit HPA database.

Gene(ID):
NODAL(4838)
Sequence:
MHAHCLPFLLHAWWALLQAGAATVATALLRTRGQPSSPSPLAYMLSLYRDPLPRADIIRSLQAEDVAVDGQNWTFAFDFSFLSQQEDLAWAELRLQLSSPVDLPTEGSLAIEIFHQPKPDTEQASDSCLERFQMDLFTVTLSQVTFSLGSMVLEVTRPLSKWLKHPGALEKQMSRVAGECWPRPPTPPATNVLLMLYSNLSQEQRQLGGSTLLWEAESSWRAQEGQLSWEWGKRHRRHHLPDRSQLCRKVKFQVDFNLIGWGSWIIYPKQYNAYRCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSMLYVDNGRVLLDHHKDMIVEECGCL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Essential for mesoderm formation and axial patterning during embryonic development.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; disulfide-linked.

Family&Domains:

Belongs to the TGF-beta family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

References

1). The Photoperiod Regulates Granulosa Cell Apoptosis through the FSH-Nodal/ALK7 Signaling Pathway in Phodopus sungorus. Animals, 2022 (PubMed: 36552491) [IF=3.0]

Application: WB    Species: Mouse    Sample: Phodopus sungorus

Figure 7. Levels of the Nodal and ACVR1C protein expression in the ovaries of Phodopus sungorus under three photoperiod conditions. (A,B) Representative immunoblots of the key Nodal and ACVR1C proteins and internal reference proteins (α-tubulin and β-actin) in three different photoperiod groups. (C,D) Relative protein expression of Nodal and ACVR1C. Values are means ± SEM. n = 8. SD, short daylight; MD, moderate daylight; LD, long daylight.

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