Product: HSD17B1 Antibody
Catalog: DF7790
Description: Rabbit polyclonal antibody to HSD17B1
Application: WB IHC IF/ICC
Reactivity: Human
Mol.Wt.: 35 kDa; 35kD(Calculated).
Uniprot: P14061
RRID: AB_2841247

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
HSD17B1 Antibody detects endogenous levels of total HSD17B1.
RRID:
AB_2841247
Cite Format: Affinity Biosciences Cat# DF7790, RRID:AB_2841247.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

17 beta HSD 1; 17 beta hydroxysteroid dehydrogenase type 1; 17-beta-HSD 1; 17-beta-hydroxysteroid dehydrogenase type 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; DHB1_HUMAN; E17KSR; E2DH; EDH17B1; EDH17B2; EDHB17; Estradiol 17 beta dehydrogenase 1; Estradiol 17-beta-dehydrogenase 1; HSD17; HSD17B1; Hydroxysteroid (17 beta) dehydrogenase 1; MGC13814; Placental 17 beta hydroxysteroid dehydrogenase; Placental 17-beta-hydroxysteroid dehydrogenase; SDR28C1; Short chain dehydrogenase/reductase family 28CE,member 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MARTVVLITGCSSGIGLHLAVRLASDPSQSFKVYATLRDLKTQGRLWEAARALACPPGSLETLQLDVRDSKSVAAARERVTEGRVDVLVCNAGLGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFLPDMKRRGSGRVLVTGSVGGLMGLPFNDVYCASKFALEGLCESLAVLLLPFGVHLSLIECGPVHTAFMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFREAAQNPEEVAEVFLTALRAPKPTLRYFTTERFLPLLRMRLDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGGGAGPGAEDEAGRGAVGDPELGDPPAAPQ

Research Backgrounds

Function:

Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Research Fields

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

References

1). The Curcumin Derivative, H10, Suppresses Hormone-Dependent Prostate Cancer by Inhibiting 17β-Hydroxysteroid Dehydrogenase Type 3. Frontiers in Pharmacology, 2020 (PubMed: 32457626) [IF=5.6]

Application: WB    Species: mouse    Sample: LC540 (17b-HSD3) cells

FIGURE 3 | Effects of H10 on 17b-HSD3 in LC540 (17b-HSD3) cells. Effects of H10 on the production of (A) T and (B) P. (C) The expression of 17b-HSD3 mRNA.(D) The expression levels of the proteins in the T synthesis pathway were detected by western blotting following treatment with H10. The concentrations of H10 were 0.25, 0.5, and 1 mM, while the concentration of 3858858 (positive control) was 0.25 mM.

Application: IHC    Species: mouse    Sample: tumor

Supplementary Figure 2. |Immunohistochemical examination of 17βHSD3 and AR expression in tumor tissue. (A) Immunohistochemical labeling of 17βHSD3-positive cells. Arrows indicate 17βHSD3 expression.

2). Ormeloxifene, a selective estrogen receptor modulator, protects against pulmonary hypertension. European Journal of Pharmacology, 2023 (PubMed: 36731722) [IF=5.0]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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