CCL22 Antibody | Affinity Biosciences

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FIGURE 6 │FKN is involved EMT and fibrosis via the Wnt/β-catenin pathway in the kidney of 760 MRL/lpr mice.

FIGURE 5 │12-week-old MRL/lpr mice were IP injected with isotype antibody, recombinant-FKN 749 protein, and anti-FKN antibody for 7 days.

Figure 1.

Product:	CCL22 Antibody
Catalog:	DF7781
Description:	Rabbit polyclonal antibody to CCL22
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	10 KD; 11kD(Calculated).
Uniprot:	O00626
RRID:	AB_2841243

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(83%), Horse(92%), Sheep(83%), Rabbit(92%), Dog(92%)

Clonality:

Polyclonal

Specificity:

CCL22 Antibody detects endogenous levels of total CCL22.

RRID:

AB_2841243
Cite Format: Affinity Biosciences Cat# DF7781, RRID:AB_2841243.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A 152E5.1; ABCD 1; ABCD1; C C motif chemokine 22; CC chemokine STCP 1; CC chemokine STCP-1; ccl 22; Ccl22; CCL22_HUMAN; Chemokine (C C motif) ligand 22; DC/B CK; DCBCK; Macrophage-derived chemokine; MDC; MDC(1-69); MDC(7-69); MGC34554; SCYA22; Small inducible cytokine subfamily A (Cys Cys) member 22; Small inducible cytokine subfamily A, member 22; Small-inducible cytokine A22; STCP 1; STCP1; Stimulated T cell chemotactic protein 1; Stimulated T-cell chemotactic protein 1;

Immunogens

Immunogen:

Uniprot:

O00626(CCL22_HUMAN) >>Visit HPA database.

Gene(ID):

CCL22(6367)

Expression:

O00626 CCL22_HUMAN:

Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis.

Sequence:

O00626 CCL22_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDRLQTALLVVLVLLAVALQATEAGPYGANMEDSVCCRDYVRYRLPLRVVKHFYWTSDSCPRPGVVLLTFRDKEICADPRVPWVKMILNKLSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Dog

Rabbit

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00626 As Substrate

O00626

Site	PTM Type	Enzyme	Source
Y54	Phosphorylation		Uniprot

Research Backgrounds

O00626_CCL22_HUMAN

Function:

May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.

PTMs:

The N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.

Subcellular Location:

Secreted.

Tissue Specificity:

Family&Domains:

Belongs to the intercrine beta (chemokine CC) family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

References

1). FKN Facilitates HK-2 Cell EMT and Tubulointerstitial Lesions via the Wnt/β-Catenin Pathway in a Murine Model of Lupus Nephritis. Frontiers in Immunology, 2019 (PubMed: 31134047) [IF=7.3]

Application: WB Species: mouse Sample: kidney

FIGURE 6 │FKN is involved EMT and fibrosis via the Wnt/β-catenin pathway in the kidney of 760 MRL/lpr mice. Histopathological features of renal tissue in the MRL/lpr mice model were studied by 761 PASM staining (original magnification, ×400). (a) Control group; (b) IgG group; (c) rFKN group; (d) 762 anti-FKN group.

Application: IHC Species: mouse Sample: renal

FIGURE 5 │12-week-old MRL/lpr mice were IP injected with isotype antibody, recombinant-FKN 749 protein, and anti-FKN antibody for 7 days. In kidney sections of MRL/lpr mice stained by IHC 750 (original magnification, ×400). (a) Control group; (b) IgG group; (c) rFKN group; (d) anti-FKN 751 group. (A1) Expression of FKN as examined by IHC staining in the renal tissues. (B1) Expression of 752 Wnt-4 as examined by IHC staining in the renal tissues. (C1) Expression of CCL22 as examined by 753 IHC staining in the renal tissues.

2). Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance. bioRxiv, 2020

Application: WB Species: Mouse Sample: gut tissue

Figure 1. Transferrin is a potential contributory factor for intestinal tolerance dependent on gut microbiota. (A) Transferrin concentration in plasma of germ-free (GF) and specific pathogen-free (SPF) (control) mice determined by ELISA. Data represent means ± SD (n = 10), *p < 0.05, **p < 0.01 by one-way ANOVA with Dunnett’s post-hoc test. Western blotting (left) and quantification (right) analyses of transferrin levels in protein extracts from liver (B) and spleen (C) of SPF and GF mice. β-actin was used as the control. Data represent means ± SD (n = 10), *p < 0.05, **p < 0.01 by one-way ANOVA with Dunnett’s post-hoc test (B, C). (D-E) Transferrin, ALDH1A2, CCL22, TGF-β, and IL-10 levels in different gut tissue segments (duodenum, jejunum, ileum, caecum, and colon (D, J, I, C1, and C2)) of SPF (NC), GF (D), and broad-spectrum antibiotic (Abs)-treated SPF (E) mice were determined by Western blotting (left) and quantification (right) analyses. GAPDH was used as the control. Data represent means ± SD (n = 8),

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CCL22 Antibody - #DF7781