SOAT1 Antibody | Affinity Biosciences

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Western blot analysis of SOAT1 using K562 whole lysates.

DF7778 at 1/100 staining Mouse liver tissue by IHC-P.

Product:	SOAT1 Antibody
Catalog:	DF7778
Description:	Rabbit polyclonal antibody to SOAT1
Application:	WB IHC
Reactivity:	Human, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	65 kDa; 65kD(Calculated).
Uniprot:	P35610
RRID:	AB_2841242

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(92%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(92%), Dog(100%), Chicken(92%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

SOAT1 Antibody detects endogenous levels of total SOAT1.

RRID:

AB_2841242
Cite Format: Affinity Biosciences Cat# DF7778, RRID:AB_2841242.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACACT; ACAT; ACAT-1; ACAT1; Acyl coenzyme A cholesterol acyltransferase 1; Acyl-coenzyme A:cholesterol acyltransferase 1; Cholesterol acyltransferase 1; SOAT; SOAT1; SOAT1_HUMAN; STAT; Sterol O acyltransferase 1; Sterol O-acyltransferase 1;

Immunogens

Immunogen:

Uniprot:

P35610(SOAT1_HUMAN) >>Visit HPA database.

Gene(ID):

SOAT1(6646)

Sequence:

P35610 SOAT1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVGEEKMSLRNRLSKSRENPEEDEDQRNPAKESLETPSNGRIDIKQLIAKKIKLTAEAEELKPFFMKEVGSHFDDFVTNLIEKSASLDNGGCALTTFSVLEGEKNNHRAKDLRAPPEQGKIFIARRSLLDELLEVDHIRTIYHMFIALLILFILSTLVVDYIDEGRLVLEFSLLSYAFGKFPTVVWTWWIMFLSTFSVPYFLFQHWATGYSKSSHPLIRSLFHGFLFMIFQIGVLGFGPTYVVLAYTLPPASRFIIIFEQIRFVMKAHSFVRENVPRVLNSAKEKSSTVPIPTVNQYLYFLFAPTLIYRDSYPRNPTVRWGYVAMKFAQVFGCFFYVYYIFERLCAPLFRNIKQEPFSARVLVLCVFNSILPGVLILFLTFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYYRTWNVVVHDWLYYYAYKDFLWFFSKRFKSAAMLAVFAVSAVVHEYALAVCLSFFYPVLFVLFMFFGMAFNFIVNDSRKKPIWNVLMWTSLFLGNGVLLCFYSQEWYARQHCPLKNPTFLDYVRPRSWTCRYVF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Pig

Zebrafish

Chicken

Rabbit

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35610 As Substrate

P35610

Site	PTM Type	Source
M1	Acetylation	Uniprot
S8	Phosphorylation	Uniprot
S14	Phosphorylation	Uniprot
S16	Phosphorylation	Uniprot
K31	Ubiquitination	Uniprot
S33	Phosphorylation	Uniprot
T36	Phosphorylation	Uniprot
S38	Phosphorylation	Uniprot
K45	Ubiquitination	Uniprot
K62	Ubiquitination	Uniprot
K67	Ubiquitination	Uniprot
S71	Phosphorylation	Uniprot
K83	Ubiquitination	Uniprot
K104	Ubiquitination	Uniprot
K120	Ubiquitination	Uniprot
K283	Ubiquitination	Uniprot
K353	Ubiquitination	Uniprot
K531	Ubiquitination	Uniprot

Research Backgrounds

P35610_SOAT1_HUMAN

Function:

Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Subunit Structure:

May form homo- or heterodimers. Interacts with UBIAD1.

Family&Domains:

Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Research Fields

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Organismal Systems > Digestive system > Cholesterol metabolism.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SOAT1 Antibody - #DF7778