Product: Histone H1 Antibody
Catalog: AF0876
Description: Rabbit polyclonal antibody to Histone H1
Application: WB IF/ICC
Reactivity: Human, Rat
Prediction: Bovine, Sheep
Mol.Wt.: 21kDa; 36kD(Calculated).
Uniprot: Q8IZA3
RRID: AB_2834405

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Bovine(80%), Sheep(80%)
Clonality:
Polyclonal
Specificity:
Histone H1 Antibody detects endogenous levels of total Histone H1.
RRID:
AB_2834405
Cite Format: Affinity Biosciences Cat# AF0876, RRID:AB_2834405.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

H1 histone family member O oocyte specific; H1FOO; H1FOO_HUMAN; Histone H1oo; Oocyte-specific histone H1; Oocyte-specific linker histone H1; osH1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q8IZA3 H1FOO_HUMAN:

Oocyte-specific.

Description:
H1FOO May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
Sequence:
MAPGSVTSDISPSSTSTAGSSRSPESEKPGPSHGGVPPGGPSHSSLPVGRRHPPVLRMVLEALQAGEQRRGTSVAAIKLYILHKYPTVDVLRFKYLLKQALATGMRRGLLARPLNSKARGATGSFKLVPKHKKKIQPRKMAPATAPRRAGEAKGKGPKKPSEAKEDPPNVGKVKKAAKRPAKVQKPPPKPGAATEKARKQGGAAKDTRAQSGEARKVPPKPDKAMRAPSSAGGLSRKAKAKGSRSSQGDAEAYRKTKAESKSSKPTASKVKNGAASPTKKKVVAKAKAPKAGQGPNTKAAAPAKGSGSKVVPAHLSRKTEAPKGPRKAGLPIKASSSKVSSQRAEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
80
Sheep
80
Rabbit
56
Pig
0
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IZA3 As Substrate

Site PTM Type Enzyme
T7 Phosphorylation
S26 Phosphorylation
R147 Methylation
K153 Acetylation
K155 Acetylation
K158 Acetylation
K205 Methylation
S245 Phosphorylation
S246 Phosphorylation
Y253 Phosphorylation

Research Backgrounds

Function:

May play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling (By similarity).

Subcellular Location:

Cytoplasm. Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Oocyte-specific.

Family&Domains:

Belongs to the histone H1/H5 family.

Restrictive clause

 

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