CR2/CD21 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/4

Western blot analysis of extracts from Rat brain, using CR2 Antibody.

Western blot analysis of extracts from VERO cells, using CR2 Antibody.

DF7770 staining HepG2 cells by IF/ICC.

Figure 5: | TT inhibited A1-type reactive astrocytes and promoted A2-type reactive astrocytes around the lesion.

Product:	CR2/CD21 Antibody
Catalog:	DF7770
Description:	Rabbit polyclonal antibody to CR2/CD21
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat, Monkey
Prediction:	Bovine, Sheep
Mol.Wt.:	155 kDa, 120 kDa; 113kD(Calculated).
Uniprot:	P20023
RRID:	AB_2841236

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Monkey

Prediction:

Bovine(100%), Sheep(90%)

Clonality:

Polyclonal

Specificity:

CR2/CD21 Antibody detects endogenous levels of total CR2/CD21.

RRID:

AB_2841236
Cite Format: Affinity Biosciences Cat# DF7770, RRID:AB_2841236.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C3DR; CD 21; CD21; Complement C3d receptor 2; Complement C3d receptor; Complement component (3d/Epstein Barr virus) receptor 2; Complement receptor type 2; CR; Cr2; CR2_HUMAN; CVID7; EBV receptor; EBV-R; Epstein Barr virus receptor; Epstein-Barr virus receptor; EVBR; SLEB9;

Immunogens

Immunogen:

Uniprot:

P20023(CR2_HUMAN) >>Visit HPA database.

Gene(ID):

CR2(1380)

Expression:

P20023 CR2_HUMAN:

Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.

Sequence:

P20023 CR2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGAAGLLGVFLALVAPGVLGISCGSPPPILNGRISYYSTPIAVGTVIRYSCSGTFRLIGEKSLLCITKDKVDGTWDKPAPKCEYFNKYSSCPEPIVPGGYKIRGSTPYRHGDSVTFACKTNFSMNGNKSVWCQANNMWGPTRLPTCVSVFPLECPALPMIHNGHHTSENVGSIAPGLSVTYSCESGYLLVGEKIINCLSSGKWSAVPPTCEEARCKSLGRFPNGKVKEPPILRVGVTANFFCDEGYRLQGPPSSRCVIAGQGVAWTKMPVCEEIFCPSPPPILNGRHIGNSLANVSYGSIVTYTCDPDPEEGVNFILIGESTLRCTVDSQKTGTWSGPAPRCELSTSAVQCPHPQILRGRMVSGQKDRYTYNDTVIFACMFGFTLKGSKQIRCNAQGTWEPSAPVCEKECQAPPNILNGQKEDRHMVRFDPGTSIKYSCNPGYVLVGEESIQCTSEGVWTPPVPQCKVAACEATGRQLLTKPQHQFVRPDVNSSCGEGYKLSGSVYQECQGTIPWFMEIRLCKEITCPPPPVIYNGAHTGSSLEDFPYGTTVTYTCNPGPERGVEFSLIGESTIRCTSNDQERGTWSGPAPLCKLSLLAVQCSHVHIANGYKISGKEAPYFYNDTVTFKCYSGFTLKGSSQIRCKADNTWDPEIPVCEKETCQHVRQSLQELPAGSRVELVNTSCQDGYQLTGHAYQMCQDAENGIWFKKIPLCKVIHCHPPPVIVNGKHTGMMAENFLYGNEVSYECDQGFYLLGEKKLQCRSDSKGHGSWSGPSPQCLRSPPVTRCPNPEVKHGYKLNKTHSAYSHNDIVYVDCNPGFIMNGSRVIRCHTDNTWVPGVPTCIKKAFIGCPPPPKTPNGNHTGGNIARFSPGMSILYSCDQGYLLVGEALLLCTHEGTWSQPAPHCKEVNCSSPADMDGIQKGLEPRKMYQYGAVVTLECEDGYMLEGSPQSQCQSDHQWNPPLAVCRSRSLAPVLCGIAAGLILLTFLIVITLYVISKHRARNYYTDTSQKEAFHLEAREVYSVDPYNPAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

Pig

Horse

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20023 As Substrate

P20023

Site	PTM Type	Source
N121	N-Glycosylation	Uniprot
N127	N-Glycosylation	Uniprot
T141	Phosphorylation	Uniprot
T145	Phosphorylation	Uniprot
S148	Phosphorylation	Uniprot
S204	Phosphorylation	Uniprot
T209	Phosphorylation	Uniprot
K216	Acetylation	Uniprot
K225	Acetylation	Uniprot
Y506	Phosphorylation	Uniprot
N623	N-Glycosylation	Uniprot
S782	Phosphorylation	Uniprot
T994	Phosphorylation	Uniprot
Y1024	Phosphorylation	Uniprot
Y1029	Phosphorylation	Uniprot

Research Backgrounds

P20023_CR2_HUMAN

Function:

Receptor for complement C3, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU. Participates in B lymphocytes activation.

(Microbial infection) Acts as a receptor for Epstein-Barr virus.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.

Subunit Structure:

Interacts (via Sushi domain 1 and 2) with C3. Interacts with CD19. Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B-cells.

(Microbial infection) Interacts with Epstein-Barr virus gp350 protein.

Family&Domains:

Belongs to the receptors of complement activation (RCA) family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)

References

1). Water Treadmill Training Ameliorates Neurite Outgrowth Inhibition Associated with NGR/RhoA/ROCK by Inhibiting Astrocyte Activation following Spinal Cord Injury. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 35387259)

Application: WB Species: rat Sample: astrocytes

Figure 5: | TT inhibited A1-type reactive astrocytes and promoted A2-type reactive astrocytes around the lesion. (a) Western blots and (b)quantification of C3d and S100A10 proteins in each group after SCI; columns represent the mean ± SD (n = 5).

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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CR2/CD21 Antibody - #DF7770