Product: CR2/CD21 Antibody
Catalog: DF7770
Description: Rabbit polyclonal antibody to CR2/CD21
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Bovine, Sheep
Mol.Wt.: 155 kDa, 120 kDa; 113kD(Calculated).
Uniprot: P20023
RRID: AB_2841236

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Bovine(100%), Sheep(90%)
Clonality:
Polyclonal
Specificity:
CR2/CD21 Antibody detects endogenous levels of total CR2/CD21.
RRID:
AB_2841236
Cite Format: Affinity Biosciences Cat# DF7770, RRID:AB_2841236.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C3DR; CD 21; CD21; Complement C3d receptor 2; Complement C3d receptor; Complement component (3d/Epstein Barr virus) receptor 2; Complement receptor type 2; CR; Cr2; CR2_HUMAN; CVID7; EBV receptor; EBV-R; Epstein Barr virus receptor; Epstein-Barr virus receptor; EVBR; SLEB9;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P20023 CR2_HUMAN:

Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.

Sequence:
MGAAGLLGVFLALVAPGVLGISCGSPPPILNGRISYYSTPIAVGTVIRYSCSGTFRLIGEKSLLCITKDKVDGTWDKPAPKCEYFNKYSSCPEPIVPGGYKIRGSTPYRHGDSVTFACKTNFSMNGNKSVWCQANNMWGPTRLPTCVSVFPLECPALPMIHNGHHTSENVGSIAPGLSVTYSCESGYLLVGEKIINCLSSGKWSAVPPTCEEARCKSLGRFPNGKVKEPPILRVGVTANFFCDEGYRLQGPPSSRCVIAGQGVAWTKMPVCEEIFCPSPPPILNGRHIGNSLANVSYGSIVTYTCDPDPEEGVNFILIGESTLRCTVDSQKTGTWSGPAPRCELSTSAVQCPHPQILRGRMVSGQKDRYTYNDTVIFACMFGFTLKGSKQIRCNAQGTWEPSAPVCEKECQAPPNILNGQKEDRHMVRFDPGTSIKYSCNPGYVLVGEESIQCTSEGVWTPPVPQCKVAACEATGRQLLTKPQHQFVRPDVNSSCGEGYKLSGSVYQECQGTIPWFMEIRLCKEITCPPPPVIYNGAHTGSSLEDFPYGTTVTYTCNPGPERGVEFSLIGESTIRCTSNDQERGTWSGPAPLCKLSLLAVQCSHVHIANGYKISGKEAPYFYNDTVTFKCYSGFTLKGSSQIRCKADNTWDPEIPVCEKETCQHVRQSLQELPAGSRVELVNTSCQDGYQLTGHAYQMCQDAENGIWFKKIPLCKVIHCHPPPVIVNGKHTGMMAENFLYGNEVSYECDQGFYLLGEKKLQCRSDSKGHGSWSGPSPQCLRSPPVTRCPNPEVKHGYKLNKTHSAYSHNDIVYVDCNPGFIMNGSRVIRCHTDNTWVPGVPTCIKKAFIGCPPPPKTPNGNHTGGNIARFSPGMSILYSCDQGYLLVGEALLLCTHEGTWSQPAPHCKEVNCSSPADMDGIQKGLEPRKMYQYGAVVTLECEDGYMLEGSPQSQCQSDHQWNPPLAVCRSRSLAPVLCGIAAGLILLTFLIVITLYVISKHRARNYYTDTSQKEAFHLEAREVYSVDPYNPAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
90
Pig
0
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20023 As Substrate

Site PTM Type Enzyme
N121 N-Glycosylation
N127 N-Glycosylation
T141 Phosphorylation
T145 Phosphorylation
S148 Phosphorylation
S204 Phosphorylation
T209 Phosphorylation
K216 Acetylation
K225 Acetylation
Y506 Phosphorylation
N623 N-Glycosylation
S782 Phosphorylation
T994 Phosphorylation
Y1024 Phosphorylation
Y1029 Phosphorylation

Research Backgrounds

Function:

Receptor for complement C3, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU. Participates in B lymphocytes activation.

(Microbial infection) Acts as a receptor for Epstein-Barr virus.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.

Subunit Structure:

Interacts (via Sushi domain 1 and 2) with C3. Interacts with CD19. Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B-cells.

(Microbial infection) Interacts with Epstein-Barr virus gp350 protein.

Family&Domains:

Belongs to the receptors of complement activation (RCA) family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

References

1). Water Treadmill Training Ameliorates Neurite Outgrowth Inhibition Associated with NGR/RhoA/ROCK by Inhibiting Astrocyte Activation following Spinal Cord Injury. Oxidative Medicine and Cellular Longevity (PubMed: 35387259)

Application: WB    Species: rat    Sample: astrocytes

Figure 5: | TT inhibited A1-type reactive astrocytes and promoted A2-type reactive astrocytes around the lesion. (a) Western blots and (b)quantification of C3d and S100A10 proteins in each group after SCI; columns represent the mean ± SD (n = 5).

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