FGF3 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from 3T3, using FGF3 Antibody.

DF7761 at 1/100 staining Mouse liver tissue by IHC-P.

Product:	FGF3 Antibody
Catalog:	DF7761
Description:	Rabbit polyclonal antibody to FGF3
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine
Mol.Wt.:	29 kDa,58kDa; 27kD(Calculated).
Uniprot:	P11487
RRID:	AB_2841227

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%)

Clonality:

Polyclonal

Specificity:

FGF3 Antibody detects endogenous levels of total FGF3.

RRID:

AB_2841227
Cite Format: Affinity Biosciences Cat# DF7761, RRID:AB_2841227.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FGF 3; Fibroblast growth factor 3; HBGF 3; HBGF3; INT 2; INT 2 proto oncogene protein; INT 2 proto oncogene protein precurosr; INT2; INT2 proto oncogene protein precurosr; Murine mammary tumor virus integration site (v int 2) oncogene homolog; Murine mammary tumor virus integration site 2; Oncogene INT2; V INT2 murine mammary tumor virus integration site oncogene homolog;

Immunogens

Immunogen:

Uniprot:

P11487(FGF3_HUMAN) >>Visit HPA database.

Gene(ID):

FGF3(2248)

Sequence:

P11487 FGF3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGLIWLLLLSLLEPGWPAAGPGARLRRDAGGRGGVYEHLGGAPRRRKLYCATKYHLQLHPSGRVNGSLENSAYSILEITAVEVGIVAIRGLFSGRYLAMNKRGRLYASEHYSAECEFVERIHELGYNTYASRLYRTVSSTPGARRQPSAERLWYVSVNGKGRPRRGFKTRRTQKSSLFLPRVLDHRDHEMVRQLQSGLPRPPGKGVQPRRRRQKQSPDNLEPSHVQASRLGSQLEASAH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Zebrafish

Xenopus

Chicken

Horse

Sheep

Dog

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11487 As Substrate

P11487

Site	PTM Type	Source
R32	Methylation	Uniprot
R44	Methylation	Uniprot
R45	Methylation	Uniprot
S93	Phosphorylation	Uniprot
Y96	Phosphorylation	Uniprot
T136	Phosphorylation	Uniprot
S138	Phosphorylation	Uniprot
S139	Phosphorylation	Uniprot
S176	Phosphorylation	Uniprot

Research Backgrounds

P11487_FGF3_HUMAN

Function:

Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal ear development.

Subcellular Location:

Secreted.

Subunit Structure:

Interacts with FGFR1 and FGFR2. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors.

Family&Domains:

Belongs to the heparin-binding growth factors family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma. (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

FGF3 Antibody - #DF7761