Nestin Antibody - #DF7754

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Product: Nestin Antibody
Catalog: DF7754
Description: Rabbit polyclonal antibody to Nestin
Application: WB IHC IF/ICC IHC-P
Reactivity: Human, Mouse, Rat
Prediction: Rabbit, Dog
Mol.Wt.: 160kD,210kD; 177kD(Calculated).
Uniprot: P48681
RRID: AB_2841220

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(93%), Dog(86%)
Clonality:
Polyclonal
Specificity:
Nestin Antibody detects endogenous levels of total Nestin.
RRID:
AB_2841220
Cite Format: Affinity Biosciences Cat# DF7754, RRID:AB_2841220.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; nes; NEST_HUMAN; Nestin;

Immunogens

Immunogen:
Uniprot:

P48681(NEST_HUMAN) >>Visit HPA database.

Gene(ID):
NES(10763)
Expression:
P48681 NEST_HUMAN:

CNS stem cells.

Sequence:
MEGCMGEESFQMWELNRRLEAYLARVKALEEQNELLSAELGGLRAQSADTSWRAHADDELAALRALVDQRWREKHAAEVARDNLAEELEGVAGRCQQLRLARERTTEEVARNRRAVEAEKCARAWLSSQVAELERELEALRVAHEEERVGLNAQAACAPRCPAPPRGPPAPAPEVEELARRLGEAWRGAVRGYQERVAHMETSLGQARERLGRAVQGAREGRLELQQLQAERGGLLERRAALEQRLEGRWQERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAHLKMSLSLEVATYRTLLEAENSRLQTPGGGSKTSLSFQDPKLELQFPRTPEGRRLGSLLPVLSPTSLPSPLPATLETPVPAFLKNQEFLQARTPTLASTPIPPTPQAPSPAVDAEIRAQDAPLSLLQTQGGRKQAPEPLRAEARVAIPASVLPGPEEPGGQRQEASTGQSPEDHASLAPPLSPDHSSLEAKDGESGGSRVFSICRGEGEGQIWGLVEKETAIEGKVVSSLQQEIWEEEDLNRKEIQDSQVPLEKETLKSLGEEIQESLKTLENQSHETLERENQECPRSLEEDLETLKSLEKENKELLKDVEVVRPLEKEAVGQLKPTGKEDTQTLQSLQKENQELMKSLEGNLETFLFPGTENQELVSSLQENLESLTALEKENQEPLRSPEVGDEEALRPLTKENQEPLRSLEDENKEAFRSLEKENQEPLKTLEEEDQSIVRPLETENHKSLRSLEEQDQETLRTLEKETQQRRRSLGEQDQMTLRPPEKVDLEPLKSLDQEIARPLENENQEFLKSLKEESVEAVKSLETEILESLKSAGQENLETLKSPETQAPLWTPEEINQGAMNPLEKEIQEPLESVEVNQETFRLLEEENQESLRSLGAWNLENLRSPEEVDKESQRNLEEEENLGKGEYQESLRSLEEEGQELPQSADVQRWEDTVEKDQELAQESPPGMAGVENEDEAELNLREQDGFTGKEEVVEQGELNATEEVWIPGEGHPESPEPKEQRGLVEGASVKGGAEGLQDPEGQSQQVGAPGLQAPQGLPEAIEPLVEDDVAPGGDQASPEVMLGSEPAMGESAAGAEPGPGQGVGGLGDPGHLTREEVMEPPLEEESLEAKRVQGLEGPRKDLEEAGGLGTEFSELPGKSRDPWEPPREGREESEAEAPRGAEEAFPAETLGHTGSDAPSPWPLGSEEAEEDVPPVLVSPSPTYTPILEDAPGPQPQAEGSQEASWGVQGRAEALGKVESEQEELGSGEIPEGPQEEGEESREESEEDELGETLPDSTPLGFYLRSPTSPRWDPTGEQRPPPQGETGKEGWDPAVLASEGLEAPPSEKEEGEEGEEECGRDSDLSEEFEDLGTEAPFLPGVPGEVAEPLGQVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPGSSVGSLQALSSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEEESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQSEEVGQGMPLVSEGDRGSPFQEEEGSALKTSWAGAPVHLGQGQFLKFTQREGDRESWSSGED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
93
Dog
86
Pig
79
Horse
79
Bovine
79
Sheep
79
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48681 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y22 Phosphorylation
K27 Ubiquitination
S37 Phosphorylation
K74 Ubiquitination
K120 Ubiquitination
K259 Ubiquitination
K271 Ubiquitination
S276 Phosphorylation
S294 Phosphorylation
S296 Phosphorylation
T304 Phosphorylation
S311 Phosphorylation
T315 Phosphorylation Q00535 (CDK5) , P06493 (CDK1)
S320 Phosphorylation
K321 Ubiquitination
T322 Phosphorylation
S323 Phosphorylation
S325 Phosphorylation
K330 Ubiquitination
T338 Phosphorylation
S346 Phosphorylation
S352 Phosphorylation
T354 Phosphorylation
S355 Phosphorylation
S358 Phosphorylation
T363 Phosphorylation
T366 Phosphorylation
T382 Phosphorylation
T384 Phosphorylation
S387 Phosphorylation
T388 Phosphorylation
T393 Phosphorylation
S398 Phosphorylation
S413 Phosphorylation
T417 Phosphorylation
S455 Phosphorylation
T456 Phosphorylation
S459 Phosphorylation
S465 Phosphorylation
S471 Phosphorylation
S475 Phosphorylation
S476 Phosphorylation
S484 Phosphorylation
S487 Phosphorylation
S491 Phosphorylation
K507 Ubiquitination
K514 Ubiquitination
S518 Phosphorylation
K532 Ubiquitination
S537 Phosphorylation
T545 Phosphorylation
S548 Phosphorylation
S556 Phosphorylation
S564 Phosphorylation
T567 Phosphorylation
S578 Phosphorylation
T585 Phosphorylation
K587 Ubiquitination
S588 Phosphorylation
S627 Phosphorylation
S638 Phosphorylation
S680 Phosphorylation
T693 Phosphorylation
S702 Phosphorylation
K708 Ubiquitination
S713 Phosphorylation
K716 Ubiquitination
S731 Phosphorylation
S743 Phosphorylation
S746 Phosphorylation
T757 Phosphorylation
S768 Phosphorylation
T776 Phosphorylation
S790 Phosphorylation
K808 Ubiquitination
S809 Phosphorylation
K811 Sumoylation
S814 Phosphorylation
K819 Ubiquitination
S820 Phosphorylation
T823 Phosphorylation
S828 Phosphorylation
K830 Ubiquitination
S831 Phosphorylation
T839 Phosphorylation
S842 Phosphorylation
T845 Phosphorylation
T851 Phosphorylation
S891 Phosphorylation
S894 Phosphorylation
S905 Phosphorylation
K911 Ubiquitination
S913 Phosphorylation
K925 Ubiquitination
Y928 Phosphorylation
S931 Phosphorylation
S934 Phosphorylation
S945 Phosphorylation
S965 Phosphorylation
S1016 Phosphorylation
S1030 Phosphorylation
S1128 Phosphorylation
S1175 Phosphorylation
Y1225 Phosphorylation
S1261 Phosphorylation
S1282 Phosphorylation
S1286 Phosphorylation
T1299 Phosphorylation Q00535 (CDK5)
S1307 Phosphorylation
S1310 Phosphorylation
S1347 Phosphorylation
S1409 Phosphorylation
S1418 Phosphorylation
S1441 Phosphorylation
S1442 Phosphorylation
S1445 Phosphorylation
S1450 Phosphorylation
S1451 Phosphorylation
S1452 Phosphorylation
S1464 Phosphorylation
S1472 Phosphorylation
S1489 Phosphorylation
S1492 Phosphorylation
S1496 Phosphorylation
S1498 Phosphorylation
S1502 Phosphorylation
S1521 Phosphorylation
S1571 Phosphorylation
S1577 Phosphorylation
S1585 Phosphorylation
T1589 Phosphorylation
S1590 Phosphorylation
K1605 Ubiquitination
S1615 Phosphorylation
S1617 Phosphorylation
S1618 Phosphorylation

Research Backgrounds

Function:

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

PTMs:

Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized (By similarity).

Tissue Specificity:

CNS stem cells.

Subunit Structure:

Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3 (By similarity).

Family&Domains:

Belongs to the intermediate filament family.

References

1). An anti-inflammatory and neuroprotective biomimetic nanoplatform for repairing spinal cord injury. Bioactive Materials, 2022 (PubMed: 35845318) [IF=18.9]
2). Neurogenesis promoted by the CD200/CD200R signaling pathway following treadmill exercise enhances post-stroke functional recovery in rats. BRAIN BEHAVIOR AND IMMUNITY, 2019 (PubMed: 31513876) [IF=15.1]

Application: IF/ICC    Species: rat    Sample: astrocytes

Fig. 5.| The expression of CD200 and CD200R was up-regulated induced by treadmill exercise after stroke in rats.The effect of treadmill exercise on the protein levels of CD200 and CD200R in the ischemic cortex (A-C) and hippocampus (D-F) at 7 and 28 days after tMCAO. CD200R staining shows protein expression at 7 and 28 days after treadmill exercise post-stroke (G). CD200R colocalizes with activated microglia (Iba1+cells, white arrows), not colocalizes with neural stem cells (Nestin+ cells) and astrocytes (GFAP+ cells), Scale bar: 50 μm. N = 4. Results are showed as means ± SEM. # P < 0.05, ## P < 0.01, ### P < 0.001 versus the Sham group. *P < 0.05, ** P < 0.01, *** P < 0.001 versus the tMCAO group.
3). Effects of RO27-3225 on neurogenesis, PDGFRβ+ cells and neuroinflammation after cerebral infarction. International Immunopharmacology, 2020 (PubMed: 32058930) [IF=5.6]

Application: IF/ICC    Species: Human    Sample: neonatal neural stem cells

Fig. 1. RO27-3225 promotes stem cell proliferation in the SVZ after cerebral infarction. (A) Immunofluorescence staining of neonatal neural stem cells (Nestin+/ BrdU+) in the SVZ on day 7 after tMCAO. (B) The quantification results showed that the mice belonging to the tMCAO + saline group had more Nestin+/BrdU+ cells in the SVZ on day 7 after tMCAO than the mice belonging to the sham + saline group, and RO27-3225 strengthened this increase (*p < 0.05 vs. the corresponding sham group; #p < 0.05 vs. the tMCAO + RO27-3225 group; n = 6/group). The data are shown as the means ± SDs.
4). miR-872-5p/FOXO3a/Wnt signaling feed-forward loop promotes proliferation of endogenous neural stem cells after spinal cord ischemia–reperfusion injury in rats. The FASEB Journal, 2023 (PubMed: 36607643) [IF=4.8]
5). Neuroprotective Action of Teriflunomide in a Mouse Model of Transient Middle Cerebral Artery Occlusion. NEUROSCIENCE, 2020 (PubMed: 31887363) [IF=3.3]
6). Establishment of an induced pluripotent stem cell line (SAHGMUi001-A) from a patient with Netherton syndrome carrying SPINK5 mutation. Stem Cell Research, 2021 (PubMed: 32416580) [IF=1.2]
7). Generation and characterization of an induced pluripotent stem cell line (ZSYYDNi001-A) from a patient with Duchenne muscular dystrophy carrying exon 51 deletion in the DMD gene. Stem Cell Research, 2021 (PubMed: 34619646) [IF=1.2]

Application: IF/ICC    Species: Human    Sample:

Fig. 1. Characterization of the induced pluripotent stem cell line.

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