ZKSCAN3 Antibody | Affinity Biosciences

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Product:	ZKSCAN3 Antibody
Catalog:	DF7752
Description:	Rabbit polyclonal antibody to ZKSCAN3
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	61 kDa; 61kD(Calculated).
Uniprot:	Q9BRR0
RRID:	AB_2841218

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

ZKSCAN3 Antibody detects endogenous levels of total ZKSCAN3.

RRID:

AB_2841218
Cite Format: Affinity Biosciences Cat# DF7752, RRID:AB_2841218.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

dJ874C20.1; mszf35; SCAN-KRAB-zinc finger protein; Skz1; Zf47; Zfp-47; ZFP306; Zfp307; Zfp47; Zinc finger and SCAN domain containing protein 13; Zinc finger and SCAN domain-containing protein 13; Zinc finger protein 306; Zinc finger protein 306, isoform CRA_a; Zinc finger protein 307; Zinc finger protein 309; Zinc finger protein 47 homolog; Zinc finger protein with KRAB and SCAN domains 3; Zinc finger protein with KRAB and SCAN domains 3; Zinc finger protein zfp47; Zinc finger with KRAB and SCAN domains 3; ZKSC3_HUMAN; ZKSCAN3; ZNF309; ZSCAN13; ZSCAN35;

Immunogens

Immunogen:

Uniprot:

Q9BRR0(ZKSC3_HUMAN) >>Visit HPA database.

Gene(ID):

ZKSCAN3(80317)

Sequence:

Q9BRR0 ZKSC3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MARELSESTALDAQSTEDQMELLVIKVEEEEAGFPSSPDLGSEGSRERFRGFRYPEAAGPREALSRLRELCRQWLQPEMHSKEQILELLVLEQFLTILPGNLQSWVREQHPESGEEVVVLLEYLERQLDEPAPQVSGVDQGQELLCCKMALLTPAPGSQSSQFQLMKALLKHESVGSQPLQDRVLQVPVLAHGGCCREDKVVASRLTPESQGLLKVEDVALTLTPEWTQQDSSQGNLCRDEKQENHGSLVSLGDEKQTKSRDLPPAEELPEKEHGKISCHLREDIAQIPTCAEAGEQEGRLQRKQKNATGGRRHICHECGKSFAQSSGLSKHRRIHTGEKPYECEECGKAFIGSSALVIHQRVHTGEKPYECEECGKAFSHSSDLIKHQRTHTGEKPYECDDCGKTFSQSCSLLEHHRIHTGEKPYQCSMCGKAFRRSSHLLRHQRIHTGDKNVQEPEQGEAWKSRMESQLENVETPMSYKCNECERSFTQNTGLIEHQKIHTGEKPYQCNACGKGFTRISYLVQHQRSHVGKNILSQ

PTMs - Q9BRR0 As Substrate

Q9BRR0

Site	PTM Type	Source
Y54	Phosphorylation	Uniprot
S65	Phosphorylation	Uniprot
K82	Methylation	Uniprot
K171	Ubiquitination	Uniprot
T207	Phosphorylation	Uniprot
T222	Phosphorylation	Uniprot
T224	Phosphorylation	Uniprot
T228	Phosphorylation	Uniprot
T337	Phosphorylation	Uniprot
T365	Phosphorylation	Uniprot
T391	Phosphorylation	Uniprot
T393	Phosphorylation	Uniprot
T421	Phosphorylation	Uniprot
T449	Phosphorylation	Uniprot
Y480	Phosphorylation	Uniprot
T503	Phosphorylation	Uniprot
K515	Acetylation	Uniprot

Research Backgrounds

Q9BRR0_ZKSC3_HUMAN

Function:

Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migration in various tumors and myelomas.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Mainly localizes in the nucleus. Under starvation conditions translocates to the cytoplasm, allowing expression of target genes involved in autophagy and lysosome biogenesis/function.

Family&Domains:

Belongs to the krueppel C2H2-type zinc-finger protein family.

References

1). Effect of salt promote the muscle triglyceride hydrolysis during dry-salting by inducing the phosphorylation of adipose tissue triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and lipid droplets splitting. Food Chemistry, 2020 (PubMed: 32454271) [IF=8.8]

Application: WB Species: Pig Sample: adipose

Fig. 3. Western blotting analysis of ATGL, HSL and their phosphorylated protein contents in adipose tissue during processing of dry-cured meat. (A) WB image of each dry-cured group. (B) ATGL relative protein level in adipose tissue. (C) p-ATGL(S406) relative protein level in adipose tissue. (D) HSL relative protein level in adipose tissue. (E) p-HSL(Ser563) relative protein level in adipose tissue. (F) p-HSL(Ser660) relative protein level in adipose tissue.

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ZKSCAN3 Antibody - #DF7752