Choline Acetyltransferase Antibody | Affinity Biosciences

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Western blot analysis of Choline Acetyltransferase using HuvEc whole lysates.

Product:	Choline Acetyltransferase Antibody
Catalog:	DF7744
Description:	Rabbit polyclonal antibody to Choline Acetyltransferase
Application:	WB
Reactivity:	Human
Mol.Wt.:	83 kDa.; 83kD(Calculated).
Uniprot:	P28329
RRID:	AB_2841211

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

Choline Acetyltransferase Antibody detects endogenous levels of total Choline Acetyltransferase.

RRID:

AB_2841211
Cite Format: Affinity Biosciences Cat# DF7744, RRID:AB_2841211.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Acetyl CoA choline O acetyltransferase; Acetyl CoA:choline O acetyltransferase; ChAT; CHOACTase; Choline acetylase; choline acetyltransferase; Choline O acetyltransferase; Choline O-acetyltransferase; CLAT_HUMAN; CMS1A; CMS1A2; EC 2.3.1.6; OTTHUMP00000019583; OTTHUMP00000019584;

Immunogens

Immunogen:

A synthesized peptide derived from human Choline Acetyltransferase, corresponding to a region within C-terminal amino acids.

Uniprot:

P28329(CLAT_HUMAN) >>Visit HPA database.

Gene(ID):

CHAT(1103)

Sequence:

P28329 CLAT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGLRTAKKRGLGGGGKWKREEGGGTRGRREVRPACFLQSGGRGDPGDVGGPAGNPGCSPHPRAATRPPPLPAHTPAHTPEWCGAASAEAAEPRRAGPHLCIPAPGLTKTPILEKVPRKMAAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHVTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

PTMs - P28329 As Substrate

P28329

Site	PTM Type	Enzyme	Source
K7	Acetylation		Uniprot
K16	Acetylation		Uniprot
K18	Acetylation		Uniprot
T25	Phosphorylation		Uniprot
T123	Phosphorylation		Uniprot
S125	Phosphorylation		Uniprot
S237	Phosphorylation		Uniprot
S244	Phosphorylation		Uniprot
T373	Phosphorylation	P17252 (PRKCA) , P05771 (PRKCB) , P05129 (PRKCG)	Uniprot
S377	Phosphorylation		Uniprot
T462	Phosphorylation		Uniprot
S464	Phosphorylation	Q02156 (PRKCE) , P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB)	Uniprot
S465	Phosphorylation	P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB) , Q02156 (PRKCE)	Uniprot
S473	Phosphorylation		Uniprot
S475	Phosphorylation		Uniprot
T553	Phosphorylation		Uniprot
Y554	Phosphorylation		Uniprot
S558	Phosphorylation	Q05513 (PRKCZ) , Q05655 (PRKCD) , P05771 (PRKCB) , Q02156 (PRKCE) , P17252 (PRKCA) , P05129 (PRKCG)	Uniprot
T574	Phosphorylation	Q9UQM7 (CAMK2A) , Q13555 (CAMK2G)	Uniprot
S594	Phosphorylation	Q02156 (PRKCE) , P05129 (PRKCG) , Q05513 (PRKCZ) , P17252 (PRKCA) , Q05655 (PRKCD) , P05771 (PRKCB)	Uniprot

Research Backgrounds

P28329_CLAT_HUMAN

Function:

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Family&Domains:

Belongs to the carnitine/choline acetyltransferase family.

Research Fields

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Organismal Systems > Nervous system > Cholinergic synapse.

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Choline Acetyltransferase Antibody - #DF7744