Product: Choline Acetyltransferase Antibody
Catalog: DF7744
Description: Rabbit polyclonal antibody to Choline Acetyltransferase
Application: WB
Reactivity: Human
Mol.Wt.: 83 kDa.; 83kD(Calculated).
Uniprot: P28329
RRID: AB_2841211

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
Choline Acetyltransferase Antibody detects endogenous levels of total Choline Acetyltransferase.
RRID:
AB_2841211
Cite Format: Affinity Biosciences Cat# DF7744, RRID:AB_2841211.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Acetyl CoA choline O acetyltransferase; Acetyl CoA:choline O acetyltransferase; ChAT; CHOACTase; Choline acetylase; choline acetyltransferase; Choline O acetyltransferase; Choline O-acetyltransferase; CLAT_HUMAN; CMS1A; CMS1A2; EC 2.3.1.6; OTTHUMP00000019583; OTTHUMP00000019584;

Immunogens

Immunogen:

A synthesized peptide derived from human Choline Acetyltransferase, corresponding to a region within C-terminal amino acids.

Uniprot:
Gene(ID):
Sequence:
MGLRTAKKRGLGGGGKWKREEGGGTRGRREVRPACFLQSGGRGDPGDVGGPAGNPGCSPHPRAATRPPPLPAHTPAHTPEWCGAASAEAAEPRRAGPHLCIPAPGLTKTPILEKVPRKMAAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHVTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

PTMs - P28329 As Substrate

Site PTM Type Enzyme
K7 Acetylation
K16 Acetylation
K18 Acetylation
T25 Phosphorylation
T123 Phosphorylation
S125 Phosphorylation
S237 Phosphorylation
S244 Phosphorylation
T373 Phosphorylation P17252 (PRKCA) , P05771 (PRKCB) , P05129 (PRKCG)
S377 Phosphorylation
T462 Phosphorylation
S464 Phosphorylation Q02156 (PRKCE) , P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB)
S465 Phosphorylation P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB) , Q02156 (PRKCE)
S473 Phosphorylation
S475 Phosphorylation
T553 Phosphorylation
Y554 Phosphorylation
S558 Phosphorylation Q05513 (PRKCZ) , Q05655 (PRKCD) , P05771 (PRKCB) , Q02156 (PRKCE) , P17252 (PRKCA) , P05129 (PRKCG)
T574 Phosphorylation Q9UQM7 (CAMK2A) , Q13555 (CAMK2G)
S594 Phosphorylation Q02156 (PRKCE) , P05129 (PRKCG) , Q05513 (PRKCZ) , P17252 (PRKCA) , Q05655 (PRKCD) , P05771 (PRKCB)

Research Backgrounds

Function:

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

Family&Domains:

Belongs to the carnitine/choline acetyltransferase family.

Research Fields

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Organismal Systems > Nervous system > Cholinergic synapse.

Restrictive clause

 

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For Research Use Only.
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