Product: BIGM103 Antibody
Catalog: DF7743
Description: Rabbit polyclonal antibody to BIGM103
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 50 kDa; 50kD(Calculated).
Uniprot: Q9C0K1
RRID: AB_2841210

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(90%), Horse(80%), Rabbit(80%), Dog(80%)
Clonality:
Polyclonal
Specificity:
BIGM103 Antibody detects endogenous levels of total BIGM103.
RRID:
AB_2841210
Cite Format: Affinity Biosciences Cat# DF7743, RRID:AB_2841210.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

4933419D20Rik; AA986696; BCG induced integral membrane protein 103; BCG induced integral membrane protein BIGMo-103 (Up-regulated by BCG-CWS) (Hypothetical protein); LIV-1 subfamily of ZIP zinc transporter 6; LZT Hs6; MGC108852; PP3105; SLC39A8; Solute carrier family 39 (zinc transporter), member 8 (SLC39A8); Solute carrier family 39 (zinc transporter), member 8; Solute carrier family 39 member 8; zinc transporter ZIP8; ZIP8; Zrt- and Irt-like protein 8;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9C0K1 S39A8_HUMAN:

Expressed in thymus, placenta, lung, liver, pancreas and, to a lower extent, in spleen, testis, ovary, small intestine, colon, leukocyte, heart. Highest expression is observed in pancreas.

Sequence:
MAPGRAVAGLLLLAAAGLGGVAEGPGLAFSEDVLSVFGANLSLSAAQLQHLLEQMGAASRVGVPEPGQLHFNQCLTAEEIFSLHGFSNATQITSSKFSVICPAVLQQLNFHPCEDRPKHKTRPSHSEVWGYGFLSVTIINLASLLGLILTPLIKKSYFPKILTFFVGLAIGTLFSNAIFQLIPEAFGFDPKVDSYVEKAVAVFGGFYLLFFFERMLKMLLKTYGQNGHTHFGNDNFGPQEKTHQPKALPAINGVTCYANPAVTEANGHIHFDNVSVVSLQDGKKEPSSCTCLKGPKLSEIGTIAWMITLCDALHNFIDGLAIGASCTLSLLQGLSTSIAILCEEFPHELGDFVILLNAGMSTRQALLFNFLSACSCYVGLAFGILVGNNFAPNIIFALAGGMFLYISLADMFPEMNDMLREKVTGRKTDFTFFMIQNAGMLTGFTAILLITLYAGEIELE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Horse
80
Dog
80
Rabbit
80
Bovine
70
Sheep
70
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9C0K1 As Substrate

Site PTM Type Enzyme
S275 Phosphorylation
S278 Phosphorylation
K284 Ubiquitination
S288 Phosphorylation
T424 Phosphorylation

Research Backgrounds

Function:

Acts as a manganese and zinc influx transporter. Plays a role in manganese reabsorption in the proximal tubule of the kidney and in manganese uptake into the brain.

Subcellular Location:

Membrane>Multi-pass membrane protein.
Note: Associated with the lysosomal/endosomal compartment following transfection.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in thymus, placenta, lung, liver, pancreas and, to a lower extent, in spleen, testis, ovary, small intestine, colon, leukocyte, heart. Highest expression is observed in pancreas.

Family&Domains:

Belongs to the ZIP transporter (TC 2.A.5) family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

References

1). Effects of Long-Term Dietary Zinc Oxide Nanoparticle on Liver Function, Deposition, and Absorption of Trace Minerals in Intrauterine Growth Retardation Pigs. BIOLOGICAL TRACE ELEMENT RESEARCH, 2022 (PubMed: 36585599) [IF=3.9]

2). Identification and analysis of a prognostic ferroptosis and iron-metabolism signature for esophageal squamous cell carcinoma. Journal of Cancer, 2023 (PubMed: 35371305) [IF=3.9]

Application: IF/ICC    Species: Human    Sample: ESCC

Figure 4 Immunofluorescence of PRNP (green), SLC39A8 (red), LCN2 (red), ATP6V0A1 (green), SLC3A2 (red), and SLC39A14 (green) in ESCC samples and normal tissues.

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