PINK1 Antibody | Affinity Biosciences

Product:	PINK1 Antibody
Catalog:	DF7742
Description:	Rabbit polyclonal antibody to PINK1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	50-70kDa; 63kD(Calculated).
Uniprot:	Q9BXM7
RRID:	AB_2841209

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Bovine(83%), Horse(94%), Sheep(89%), Dog(94%)

Clonality:

Polyclonal

Specificity:

PINK1 Antibody detects endogenous levels of total PINK1.

RRID:

AB_2841209
Cite Format: Affinity Biosciences Cat# DF7742, RRID:AB_2841209.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BRPK; FLJ27236; mitochondrial; PARK 6; PARK6; Phosphatase and Tensin Homolog; PINK 1; PINK1; PINK1_HUMAN; Protein kinase BRPK; PTEN induced putative kinase 1; PTEN induced putative kinase protein 1; PTEN-induced putative kinase protein 1; Serine/threonine kinase PINK1 mitochondrial; Serine/threonine protein kinase PINK1 mitochondrial; Serine/threonine-protein kinase PINK1;

Immunogens

Immunogen:

Uniprot:

Q9BXM7(PINK1_HUMAN) >>Visit HPA database.

Gene(ID):

PINK1(65018)

Expression:

Q9BXM7 PINK1_HUMAN:

Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.

Sequence:

Q9BXM7 PINK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAAGPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Dog

Pig

Sheep

Bovine

Rabbit

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BXM7 As Substrate

Q9BXM7

Site	PTM Type	Enzyme	Source
K137	Ubiquitination		Uniprot
K164	Ubiquitination		Uniprot
K186	Ubiquitination		Uniprot
S228	Phosphorylation	Q9BXM7 (PINK1)	Uniprot
S229	Phosphorylation		Uniprot
S230	Phosphorylation		Uniprot
T257	Phosphorylation	Q9BXM7 (PINK1)	Uniprot
K262	Acetylation		Uniprot
S284	Phosphorylation		Uniprot
T313	Phosphorylation	Q7KZI7 (MARK2)	Uniprot
K319	Ubiquitination		Uniprot
S402	Phosphorylation	Q9BXM7 (PINK1)	Uniprot
K433	Ubiquitination		Uniprot
S465	Phosphorylation		Uniprot
S495	Phosphorylation		Uniprot
K547	Ubiquitination		Uniprot

PTMs - Q9BXM7 As Enzyme

Q9BXM7

Substrate	Site	Source
O15379 (HDAC3)	S424	Uniprot
O43464 (HTRA2)	S142	Uniprot
O60260 (PRKN)	S65	Uniprot
P0CG48 (UBC)	S65	Uniprot
Q8IXI2 (RHOT1)	S156	Uniprot
Q9BXM7 (PINK1)	S228	Uniprot
Q9BXM7 (PINK1)	T257	Uniprot
Q9BXM7 (PINK1)	S402	Uniprot
Q9Y2N7 (HIF3A)	T12	Uniprot

Research Backgrounds

Q9BXM7_PINK1_HUMAN

Function:

Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN. Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form. Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (By similarity).

PTMs:

Autophosphorylation at Ser-228 and Ser-402 is essential for Parkin/PRKN recruitment to depolarized mitochondria.

Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol. Processed into a 52 kDa mature form by PARL or AFG3L2 following the cleavage by mitochondrial-processing peptidase (MPP) during mitochondrial import.

Subcellular Location:

Mitochondrion outer membrane>Single-pass membrane protein. Mitochondrion inner membrane>Single-pass membrane protein. Cytoplasm>Cytosol.
Note: Localizes mostly in mitochondrion and the 2 proteolytic processed fragments of 55 kDa and 48 kDa localize mainly in cytosol.

Tissue Specificity:

Subunit Structure:

Interacts with PRKN. Interacts with FBXO7. Forms a complex with PRKN and PARK7. Interacts with NENF.

Family&Domains:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

References

1). TRPM2 protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction via modulating autophagy. Theranostics, 2023 (PubMed: 37649595) [IF=12.4]

Application: WB Species: Mouse Sample: kidney

Figure 9 TRPM2-mediated Ca2+ influx is involved in the inhibition of the AKT-mTOR pathway in response to cisplatin. Intracellular Ca2+ signals indicated by the fluorescence of Fluo-4 AM in cisplatin (CP, 20 μM) or normal saline (NS) treated Trpm2-/- and WT MEFs (A, B) and mRTECs (C, D) (n = 7). Scale bars, 20 μm and 10μm, respectively. (E) Immunoblotting analysis and quantification of LC3B II in WT MEFs after incubation with clotrimazole (CLT, 10 μM) or vehicle (Veh) for 1 h followed by treatment with CP at the indicated concentration for 24 h. (F) Immunoblotting analysis and quantification of LC3B II in WT MEFs after incubation with 5 μM BAPTA-AM for 1 h followed by treatment with 20 μM CP for 24 h. (G) Immunoblotting analysis and quantification of p-AKT, AKT and p-p70S6K in WT MEFs after incubation with 5 μM BAPTA-AM or 10 μM clotrimazole for 1 h followed by treatment with 20 μM CP for 24 h. (H, I) Mitochondrial Ca2+ signals indicated by the fluorescence of Rhod-2 in mRTECs treated by CP or NS (n = 7). Scale bars, 10 μm. (J) Immunoblotting analysis and quantification of BNIP3 and PINK1 in mRTECs treated by CP or NS. Data are presented as mean±SEM. Statistical analysis was performed using one-way ANOVA with Tukey post-hoc test. ns, not significant; *p < 0.05, **p < 0.01, ***p < 0.001.

2). Cooperative STAT3-NFkB signaling modulates mitochondrial dysfunction and metabolic profiling in hepatocellular carcinoma. Metabolism: clinical and experimental, 2024 (PubMed: 38184165) [IF=9.8]

3). Integrating network analysis and experimental validation to reveal the mitophagy-associated mechanism of Yiqi Huoxue (YQHX) prescription in the treatment of myocardial ischemia/reperfusion injury. PHARMACOLOGICAL RESEARCH, 2023 (PubMed: 36736970) [IF=9.3]

4). Activation of aldehyde dehydrogenase-2 improves ischemic random skin flap survival in rats. Frontiers in Immunology, 2023 (PubMed: 37441072) [IF=7.3]

Application: IHC Species: Mouse Sample:

Figure 9 (A) Immunohistochemistry images of ALDH2, PINK1 and Parkin. All images were obtained at identical magnification, ×200, scale bar = 50 μm. (B) Quantitative analysis of ALDH2, PINK1 and Parkin content (n = 3). Data are represented as mean ± SEM. **P

5). Polystyrene microplastics induce mitochondrial damage in mouse GC-2 cells. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022 (PubMed: 35489138) [IF=6.8]

Application: WB Species: mouse Sample: GC-2 cells

Fig. 2.| Effects of PS-MPS on mitochondrial membrane protein and membrane potential in control group and PS-MPS group after 24 h. (A) Relative transcription of mRNA of PINK1; (B) Relative protein expression of PINK1

6). 1, 3-Dichloro-2-propanol-Induced Renal Tubular Cell Necroptosis through the ROS/RIPK3/MLKL Pathway. Journal of Agricultural and Food Chemistry, 2022 (PubMed: 36000575) [IF=6.1]

7). 3-MCPD Induces Renal Cell Pyroptosis and Inflammation by Inhibiting ESCRT-III-Mediated Cell Repair and Mitophagy. Journal of agricultural and food chemistry, 2024 (PubMed: 38857427) [IF=6.1]

8). Walnut-Derived Peptide Enhances Mitophagy via JNK-Mediated PINK1 Activation to Reduce Oxidative Stress in HT-22 Cells. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2022 (PubMed: 35187930) [IF=6.1]

9). Effect of Thyroxine on the Structural and Dynamic Features of Cardiac Mitochondria and Mitophagy in Rats. Cells, 2023 (PubMed: 36766738) [IF=6.0]

10). Structural and Dynamic Features of Liver Mitochondria and Mitophagy in Rats with Hyperthyroidism. International Journal of Molecular Sciences, 2022 (PubMed: 36430802) [IF=5.6]

Application: WB Species: Rat Sample: liver tissue

Figure 6 Immunoblotting analysis of mitophagy proteins in liver tissue of control and hyperthyroid rats. (A) Representative Western blot of Parkin, PINK1, SQSTM1/p62, LC3A/B-I:II, BNIP3L, C1-C5-CR, T1-T5-HR. (B–F) Relative levels of appropriate proteins with respect to the loading control (GAPDH). CR, control; HR, hyperthyroidism. **—p < 0.02, ***—p < 0.001 as compared with the control data (n = 5–6).

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PINK1 Antibody - #DF7742