GDNF Antibody | Affinity Biosciences

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Product:	GDNF Antibody
Catalog:	DF7727
Description:	Rabbit polyclonal antibody to GDNF
Application:	WB
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	24 kDa; 24kD(Calculated).
Uniprot:	P39905
RRID:	AB_2841195

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(80%), Dog(82%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

GDNF Antibody detects endogenous levels of total GDNF.

RRID:

AB_2841195
Cite Format: Affinity Biosciences Cat# DF7727, RRID:AB_2841195.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Astrocyte derived trophic factor; Astrocyte derived trophic factor 1; Astrocyte-derived trophic factor; ATF 1; ATF 2; Atf; ATF1; ATF2; gdnf; GDNF_HUMAN; Glial cell derived neurotrophic factor; Glial Cell Line Derived Neurotrophic Factor; Glial cell line-derived neurotrophic factor; Glial derived neurotrophic factor; HFB1 GDNF; hGDNF; HSCR3;

Immunogens

Immunogen:

Uniprot:

P39905(GDNF_HUMAN) >>Visit HPA database.

Gene(ID):

GDNF(2668)

Expression:

P39905 GDNF_HUMAN:

In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.

Sequence:

P39905 GDNF_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKLWDVVAVCLVLLHTASAFPLPAGKRPPEAPAEDRSLGRRRAPFALSSDSNMPEDYPDQFDDVMDFIQATIKRLKRSPDKQMAVLPRRERNRQAAAANPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Chicken

100

Pig

Dog

Rabbit

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P39905 As Substrate

P39905

Site	PTM Type	Source
N126	N-Glycosylation	Uniprot
S146	Phosphorylation	Uniprot
S148	Phosphorylation	Uniprot
T154	Phosphorylation	Uniprot
T155	Phosphorylation	Uniprot
Y156	Phosphorylation	Uniprot
S164	Phosphorylation	Uniprot

Research Backgrounds

P39905_GDNF_HUMAN

Function:

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Subcellular Location:

Secreted.

Tissue Specificity:

Subunit Structure:

Homodimer; disulfide-linked. Interacts with RET. Interacts (via propeptide) with SORL1 (via N-terminal ectodomain); this interaction affects GDNF-regulated, but not constitutive secretion. Also interacts with SORL1 in complex with GFRA1; this interaction leads to GDNF endocytosis and lysosomal degradation.

Family&Domains:

Belongs to the TGF-beta family. GDNF subfamily.

References

1). Recurrent non-severe hypoglycemia aggravates cognitive decline in diabetes and induces mitochondrial dysfunction in cultured astrocytes. Molecular and Cellular Endocrinology, 2021 (PubMed: 33545179) [IF=4.1]

Application: WB Species: mice Sample: cortex tissue

Fig. 3. Recurrent non-severe hypoglycemia (RH) inhibits brain-derived neurotrophic factor (BDNF) and glial cell derived neurotrophic factor (GDNF) signaling in diabetic mice. (A) The expression of BDNF/tropomyosin (t)-receptor (r)-kinase (k) B (TrkB) signaling and GDNF/GDNF family receptor alpha-1 (GFRa1)/receptor tyrosine kinase (Ret) signaling in mice cerebral cortex, measured using western blotting. (B–G) Relative protein levels of BDNF, TrkB, p-TrkB, GDNF, GFRa1, Ret, and p-Ret (Tyr1062), as determined by ImageJ. *P < 0.05 vs. normal control (NC), **P < 0.01 vs. NC, #P < 0.05 vs. diabetes mellitus (DM), ##P < 0.05 vs. DM.

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GDNF Antibody - #DF7727