Product: GDNF Antibody
Catalog: DF7727
Description: Rabbit polyclonal antibody to GDNF
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 24 kDa; 24kD(Calculated).
Uniprot: P39905
RRID: AB_2841195

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(80%), Dog(82%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
GDNF Antibody detects endogenous levels of total GDNF.
RRID:
AB_2841195
Cite Format: Affinity Biosciences Cat# DF7727, RRID:AB_2841195.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Astrocyte derived trophic factor; Astrocyte derived trophic factor 1; Astrocyte-derived trophic factor; ATF 1; ATF 2; Atf; ATF1; ATF2; gdnf; GDNF_HUMAN; Glial cell derived neurotrophic factor; Glial Cell Line Derived Neurotrophic Factor; Glial cell line-derived neurotrophic factor; Glial derived neurotrophic factor; HFB1 GDNF; hGDNF; HSCR3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P39905 GDNF_HUMAN:

In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.

Sequence:
MKLWDVVAVCLVLLHTASAFPLPAGKRPPEAPAEDRSLGRRRAPFALSSDSNMPEDYPDQFDDVMDFIQATIKRLKRSPDKQMAVLPRRERNRQAAAANPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Chicken
100
Pig
82
Dog
82
Rabbit
80
Xenopus
60
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P39905 As Substrate

Site PTM Type Enzyme
N126 N-Glycosylation
S146 Phosphorylation
S148 Phosphorylation
T154 Phosphorylation
T155 Phosphorylation
Y156 Phosphorylation
S164 Phosphorylation

Research Backgrounds

Function:

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.

Subunit Structure:

Homodimer; disulfide-linked. Interacts with RET. Interacts (via propeptide) with SORL1 (via N-terminal ectodomain); this interaction affects GDNF-regulated, but not constitutive secretion. Also interacts with SORL1 in complex with GFRA1; this interaction leads to GDNF endocytosis and lysosomal degradation.

Family&Domains:

Belongs to the TGF-beta family. GDNF subfamily.

References

1). Recurrent non-severe hypoglycemia aggravates cognitive decline in diabetes and induces mitochondrial dysfunction in cultured astrocytes. Molecular and Cellular Endocrinology, 2021 (PubMed: 33545179) [IF=4.1]

Application: WB    Species: mice    Sample: cortex tissue

Fig. 3. Recurrent non-severe hypoglycemia (RH) inhibits brain-derived neurotrophic factor (BDNF) and glial cell derived neurotrophic factor (GDNF) signaling in diabetic mice. (A) The expression of BDNF/tropomyosin (t)-receptor (r)-kinase (k) B (TrkB) signaling and GDNF/GDNF family receptor alpha-1 (GFRa1)/receptor tyrosine kinase (Ret) signaling in mice cerebral cortex, measured using western blotting. (B–G) Relative protein levels of BDNF, TrkB, p-TrkB, GDNF, GFRa1, Ret, and p-Ret (Tyr1062), as determined by ImageJ. *P < 0.05 vs. normal control (NC), **P < 0.01 vs. NC, #P < 0.05 vs. diabetes mellitus (DM), ##P < 0.05 vs. DM.

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For Research Use Only.
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