Aquaporin 7 Antibody - #DF7717

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Product: Aquaporin 7 Antibody
Catalog: DF7717
Description: Rabbit polyclonal antibody to Aquaporin 7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Dog
Mol.Wt.: 37 kDa; 37kD(Calculated).
Uniprot: O14520
RRID: AB_2841186

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Dog(82%)
Clonality:
Polyclonal
Specificity:
Aquaporin 7 Antibody detects endogenous levels of total Aquaporin 7.
RRID:
AB_2841186
Cite Format: Affinity Biosciences Cat# DF7717, RRID:AB_2841186.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AQP 7; AQP 7L; AQP 9; AQP-7; Aqp7; AQP7_HUMAN; AQP7L; AQP9; AQPap; Aquaglyceroporin-7; Aquaporin 7 like; Aquaporin adipose; Aquaporin-7; Aquaporin-7-like; Aquaporin7; MGC149555; MGC149556;

Immunogens

Immunogen:
Uniprot:

O14520(AQP7_HUMAN) >>Visit HPA database.

Gene(ID):
AQP7(364)
Expression:
O14520 AQP7_HUMAN:

Detected in the sperm head (at protein level) (PubMed:28042826). Detected in white adipose tissue (PubMed:9405233).

Sequence:
MVQASGHRRSTRGSKMVSWSVIAKIQEILQRKMVREFLAEFMSTYVMMVFGLGSVAHMVLNKKYGSYLGVNLGFGFGVTMGVHVAGRISGAHMNAAVTFANCALGRVPWRKFPVYVLGQFLGSFLAAATIYSLFYTAILHFSGGQLMVTGPVATAGIFATYLPDHMTLWRGFLNEAWLTGMLQLCLFAITDQENNPALPGTEALVIGILVVIIGVSLGMNTGYAINPSRDLPPRIFTFIAGWGKQVFSNGENWWWVPVVAPLLGAYLGGIIYLVFIGSTIPREPLKLEDSVAYEDHGITVLPKMGSHEPTISPLTPVSVSPANRSSVHPAPPLHESMALEHF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
82
Rabbit
60
Pig
0
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14520 As Substrate

Site PTM Type Enzyme
S18 Phosphorylation
S20 Phosphorylation
S306 Phosphorylation

Research Backgrounds

Function:

Forms a channel that mediates water and glycerol transport across cell membranes at neutral pH. The channel is also permeable to urea. Plays an important role in body energy homeostasis under conditions that promote lipid catabolism, giving rise to glycerol and free fatty acids. Mediates glycerol export from adipocytes. After release into the blood stream, glycerol is used for gluconeogenesis in the liver to maintain normal blood glucose levels and prevent fasting hypoglycemia. Required for normal glycerol reabsorption in the kidney (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cytoplasm>Cell cortex. Cytoplasmic vesicle membrane>Multi-pass membrane protein. Lipid droplet.
Note: Internalized from the cell membrane in response to catecholamine-induced activation of PKA; detected on intracellular membranes and colocalizes with lipid droplets (By similarity). Colocalizes with PLIN1 in adipocytes, probably on lipid droplets (PubMed:27832861).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in the sperm head (at protein level). Detected in white adipose tissue.

Subunit Structure:

Homotetramer (Probable). Interacts (via N-terminus) with PLIN1.

Family&Domains:

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro/Ala-Ala/Ser (NPA).

Belongs to the MIP/aquaporin (TC 1.A.8) family.

Research Fields

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

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