FATP1 Antibody - #DF7716

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Product: FATP1 Antibody
Catalog: DF7716
Description: Rabbit polyclonal antibody to FATP1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 65 kDa; 71kD(Calculated).
Uniprot: Q6PCB7
RRID: AB_2841185

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(92%), Sheep(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
FATP1 Antibody detects endogenous levels of total FATP1.
RRID:
AB_2841185
Cite Format: Affinity Biosciences Cat# DF7716, RRID:AB_2841185.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACSVL5; FATP 1; FATP; FATP1; Fatty acid transport protein 1; FLJ00336; Long chain fatty acid transport protein 1; MGC71751; SLC27A1; Solute carrier family 27 (fatty acid transporter) member 1; Solute carrier family 27 member 1;

Immunogens

Immunogen:
Uniprot:

Q6PCB7(S27A1_HUMAN) >>Visit HPA database.

Gene(ID):
SLC27A1(376497)
Expression:
Q6PCB7 S27A1_HUMAN:

Highest levels of expression are detected in muscle and adipose tissue small, intermediate levels in small intestine, and barely detectable in liver.

Sequence:
MRAPGAGAASVVSLALLWLLGLPWTWSAAAALGVYVGSGGWRFLRIVCKTARRDLFGLSVLIRVRLELRRHQRAGHTIPRIFQAVVQRQPERLALVDAGTGECWTFAQLDAYSNAVANLFRQLGFAPGDVVAIFLEGRPEFVGLWLGLAKAGMEAALLNVNLRREPLAFCLGTSGAKALIFGGEMVAAVAEVSGHLGKSLIKFCSGDLGPEGILPDTHLLDPLLKEASTAPLAQIPSKGMDDRLFYIYTSGTTGLPKAAIVVHSRYYRMAAFGHHAYRMQAADVLYDCLPLYHSAGNIIGVGQCLIYGLTVVLRKKFSASRFWDDCIKYNCTVVQYIGEICRYLLKQPVREAERRHRVRLAVGNGLRPAIWEEFTERFGVRQIGEFYGATECNCSIANMDGKVGSCGFNSRILPHVYPIRLVKVNEDTMELLRDAQGLCIPCQAGEPGLLVGQINQQDPLRRFDGYVSESATSKKIAHSVFSKGDSAYLSGDVLVMDELGYMYFRDRSGDTFRWRGENVSTTEVEGVLSRLLGQTDVAVYGVAVPGVEGKAGMAAVADPHSLLDPNAIYQELQKVLAPYARPIFLRLLPQVDTTGTFKIQKTRLQREGFDPRQTSDRLFFLDLKQGHYLPLNEAVYTRICSGAFAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Chicken
100
Horse
92
Pig
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6PCB7 As Substrate

Site PTM Type Enzyme
K202 Ubiquitination
K225 Ubiquitination
K238 Acetylation
Y246 Phosphorylation
Y248 Phosphorylation
Y266 Phosphorylation
Y267 Phosphorylation
S468 Phosphorylation
K474 Ubiquitination
K475 Ubiquitination
Y501 Phosphorylation

Research Backgrounds

Function:

Mediates the ATP-dependent import of long-chain fatty acids (LCFA) into the cell by mediating their translocation at the plasma membrane. Has also an acyl-CoA ligase activity for long-chain and very-long-chain fatty acids. May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation. Plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis. May be involved in regulation of cholesterol metabolism (By similarity).

Subcellular Location:

Cell membrane>Single-pass membrane protein. Endomembrane system>Single-pass membrane protein. Cytoplasm.
Note: Plasma membrane and intracellular membranes, at least in adipocytes. In adipocytes, but not myocytes, insulin via the mTORC1 signaling pathway induces a rapid translocation of SLC27A1 from intracellular compartments to the plasma membrane, paralleled by increased LCFA uptake. Insulin-dependent translocation from the cytoplasm to the cell membrane is regulated by EPRS1. Predominantly cytoplasmic in myocytes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest levels of expression are detected in muscle and adipose tissue small, intermediate levels in small intestine, and barely detectable in liver.

Subunit Structure:

Self-associates. May function as a homodimer (By similarity). Interacts with EPRS1; mediates the translocation of SLC27A1 from the cytoplasm to the plasma membrane thereby increasing the uptake of long-chain fatty acids.

Family&Domains:

Belongs to the ATP-dependent AMP-binding enzyme family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

References

1). Downregulations of placental fatty acid transporters during cadmium-induced fetal growth restriction. Toxicology, 2019 (PubMed: 31152847) [IF=4.5]

Application: WB    Species: mouse    Sample: placenta

Fig. 4. |Effects of maternal Cd exposure on the protein expression of placental FATP1, FATP6 and FABP3. (A): FATP1, FATP6 and FABP3 at E16.55; (B): FATP1, FATP6 and FABP3 at E19.5. Each sample group composed of 5 mice. For each mouse, one placenta was taken for protein analysis. The number of samples in each groupfor western blotting assay was 5. E: embryonic day. Results were presented as mean ± SD. *:p < 0.05; compared with the controls.
2). Severe hypoglycemia exacerbates myocardial dysfunction and metabolic remodeling in diabetic mice. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2020 (PubMed: 31887336) [IF=4.1]

Application: WB    Species: mouse    Sample: myocardial

Fig. 6. |SH inhibited myocardial metabolism in diabetic mice. Real-time PCR analysis of the gene expression involved in myocardial fatty acid transportation,oxidation, and glucose uptake (n = 8 per group). (B) Gene expression of myocardial transcriptional regulator PPARs (n = 8 per group). (C) Representative immunoblot images of relative protein expression (n = 6 per group).

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