Renin Antibody | Affinity Biosciences

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Western blot analysis of Renin using COLO205 whole lysates.

Product:	Renin Antibody
Catalog:	DF7714
Description:	Rabbit polyclonal antibody to Renin
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig
Mol.Wt.:	45 kDa; 45kD(Calculated).
Uniprot:	P00797
RRID:	AB_2841183

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(80%)

Clonality:

Polyclonal

Specificity:

Renin Antibody detects endogenous levels of total Renin.

RRID:

AB_2841183
Cite Format: Affinity Biosciences Cat# DF7714, RRID:AB_2841183.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Angiotensin forming enzyme; Angiotensin forming enzyme precursor; Angiotensinogenase; Angiotensinogenase precursor; FLJ10761; HNFJ2; REN; Ren1; RENI_HUMAN; Renin; Renin precursor renal;

Immunogens

Immunogen:

Uniprot:

P00797(RENI_HUMAN) >>Visit HPA database.

Gene(ID):

REN(5972)

Sequence:

P00797 RENI_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDGWRRMPRWGLLLLLWGSCTFGLPTDTTTFKRIFLKRMPSIRESLKERGVDMARLGPEWSQPMKRLTLGNTTSSVILTNYMDTQYYGEIGIGTPPQTFKVVFDTGSSNVWVPSSKCSRLYTACVYHKLFDASDSSSYKHNGTELTLRYSTGTVSGFLSQDIITVGGITVTQMFGEVTEMPALPFMLAEFDGVVGMGFIEQAIGRVTPIFDNIISQGVLKEDVFSFYYNRDSENSQSLGGQIVLGGSDPQHYEGNFHYINLIKTGVWQIQMKGVSVGSSTLLCEDGCLALVDTGASYISGSTSSIEKLMEALGAKKRLFDYVVKCNEGPTLPDISFHLGGKEYTLTSADYVFQESYSSKKLCTLAIHAMDIPPPTGPTWALGATFIRKFYTEFDRRNNRIGFALAR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00797 As Substrate

P00797

Site	PTM Type	Source
S41	Phosphorylation	Uniprot
S45	Phosphorylation	Uniprot
Y87	Phosphorylation	Uniprot
T330	Phosphorylation	Uniprot
S347	Phosphorylation	Uniprot
S355	Phosphorylation	Uniprot

Research Backgrounds

P00797_RENI_HUMAN

Function:

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.

Subcellular Location:

Secreted. Membrane.
Note: Associated to membranes via binding to ATP6AP2.

Subunit Structure:

Interacts with ATP6AP2.

Family&Domains:

Belongs to the peptidase A1 family.

Research Fields

· Organismal Systems > Endocrine system > Renin-angiotensin system. (View pathway)

· Organismal Systems > Endocrine system > Renin secretion.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Renin Antibody - #DF7714