67kDa Laminin Receptor Antibody - #DF7710

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Product: 67kDa Laminin Receptor Antibody
Catalog: DF7710
Description: Rabbit polyclonal antibody to 67kDa Laminin Receptor
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 33 kDa; 33kD(Calculated).
Uniprot: P08865
RRID: AB_2841179

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(92%), Zebrafish(100%), Bovine(92%), Horse(100%), Sheep(83%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
67kDa Laminin Receptor Antibody detects endogenous levels of total 67kDa Laminin Receptor.
RRID:
AB_2841179
Cite Format: Affinity Biosciences Cat# DF7710, RRID:AB_2841179.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

34/67 kDa laminin receptor; 37 kDa laminin receptor precursor; 37/67 kDa laminin receptor; 37LRP; 40S ribosomal protein SA; 67 kDa laminin receptor; 67LR; Colon carcinoma laminin binding protein; Colon carcinoma laminin-binding protein; LAMBR; Laminin receptor 1; Laminin-binding protein precursor p40; LAMR 1; LamR; LAMR1; LBP; LBP/p40; LRP; LRP/LR; Multidrug resistance associated protein MGr1 Ag; Multidrug resistance associated protein MGr1Ag; Multidrug resistance-associated protein MGr1-Ag; NEM/1CHD4; p40; Ribosomal Protein SA; rpsA; RSSA_HUMAN; SA;

Immunogens

Immunogen:
Uniprot:

P08865(RSSA_HUMAN) >>Visit HPA database.

Gene(ID):
RPSA(3921)
Sequence:
MSGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSRNTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRYVDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTATQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTDWS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
92
Bovine
92
Sheep
83
Xenopus
77
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08865 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K11 Ubiquitination
T28 Phosphorylation
Y37 Phosphorylation
Y39 Phosphorylation
K40 Acetylation
K40 Methylation
K40 Ubiquitination
K42 Ubiquitination
S43 Phosphorylation
Y47 Phosphorylation
K52 Acetylation
K52 Ubiquitination
K57 Acetylation
K57 Ubiquitination
S79 Phosphorylation
K89 Acetylation
K89 Methylation
K89 Ubiquitination
T97 Phosphorylation
T104 Phosphorylation
T125 Phosphorylation
T135 Phosphorylation
S138 Phosphorylation
Y139 Phosphorylation
T150 Phosphorylation
Y156 Phosphorylation
C163 S-Nitrosylation
S170 Phosphorylation
R180 Methylation
Y204 Phosphorylation
K212 Sumoylation
K220 Ubiquitination
K224 Sumoylation
T239 Phosphorylation
T241 Phosphorylation
S249 Phosphorylation
S268 Phosphorylation

Research Backgrounds

Function:

Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA.

(Microbial infection) Acts as a receptor for the Adeno-associated viruses 2,3,8 and 9.

(Microbial infection) Acts as a receptor for the Dengue virus.

(Microbial infection) Acts as a receptor for the Sindbis virus.

(Microbial infection) Acts as a receptor for the Venezuelan equine encephalitis virus.

(Microbial infection) Acts as a receptor for the pathogenic prion protein.

(Microbial infection) Acts as a receptor for bacteria.

PTMs:

Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.

Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.

Subcellular Location:

Cell membrane. Cytoplasm. Nucleus.
Note: 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Colocalizes with PPP1R16B in the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer (37LRP) and homodimer (67LR). Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains about 33 different proteins and 1 molecule of RNA (18S). The 60S subunit contains about 49 different proteins and 3 molecules of RNA (28S, 5.8S and 5S). Interacts with RPS21. Interacts with several laminins including at least LAMB1. Interacts with MDK (By similarity). The mature dimeric form interacts with PPP1R16B (via its fourth ankyrin repeat). Interacts with PPP1CA only in the presence of PPP1R16B.

(Microbial infection) 67LR interacts with capsid protein of Adeno-associated virus 2,3,8 and 9.

(Microbial infection) 67LR interacts with envelope protein of dengue virus.

(Microbial infection) 6s7LR interacts with E2 glycoprotein of Sindbis and Venezuelan equine encephalitis virus.

Family&Domains:

Belongs to the universal ribosomal protein uS2 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome.

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