Ferritin Heavy Chain Antibody | Affinity Biosciences

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Western blot analysis of Ferritin Heavy Chain using K562 whole lysates.

Product:	Ferritin Heavy Chain Antibody
Catalog:	DF7705
Description:	Rabbit polyclonal antibody to Ferritin Heavy Chain
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.:	21 kDa; 21kD(Calculated).
Uniprot:	P02794
RRID:	AB_2841175

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(88%), Bovine(88%), Horse(86%), Rabbit(88%), Dog(88%), Xenopus(88%)

Clonality:

Polyclonal

Specificity:

Ferritin Heavy Chain Antibody detects endogenous levels of total Ferritin Heavy Chain.

RRID:

AB_2841175
Cite Format: Affinity Biosciences Cat# DF7705, RRID:AB_2841175.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Apoferritin; Cell proliferation inducing gene 15 protein; Cell proliferation-inducing gene 15 protein; F HC; Ferritin H subunit; Ferritin heavy chain; Ferritin heavy polypeptide 1; FHC; FRIH; FRIH_HUMAN; FTH 1; FTH; FTH1; FTH1 protein; FTHL 6; FTHL6; Iron overload autosomal dominant; MGC104426; N-terminally processed; OK/SW-cl.84; PIG 15; PIG15; Placenta immunoregulatory factor; PLIF; Proliferation inducing gene 15 protein; Proliferation inducing protein 15;

Immunogens

Immunogen:

Uniprot:

P02794(FRIH_HUMAN) >>Visit HPA database.

Gene(ID):

FTH1(2495)

Expression:

P02794 FRIH_HUMAN:

Expressed in the liver.

Sequence:

P02794 FRIH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Dog

Xenopus

Rabbit

Horse

Sheep

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02794 As Substrate

P02794

Site	PTM Type	Source
M1	Acetylation	Uniprot
T2	Acetylation	Uniprot
T6	Phosphorylation	Uniprot
S7	Phosphorylation	Uniprot
Y13	Phosphorylation	Uniprot
K54	Ubiquitination	Uniprot
K69	Ubiquitination	Uniprot
K72	Sumoylation	Uniprot
K72	Ubiquitination	Uniprot
K88	Ubiquitination	Uniprot
S96	Phosphorylation	Uniprot
K109	Ubiquitination	Uniprot
S114	Phosphorylation	Uniprot
K120	Ubiquitination	Uniprot
K125	Ubiquitination	Uniprot
K144	Ubiquitination	Uniprot
K147	Ubiquitination	Uniprot
K158	Ubiquitination	Uniprot
S164	Phosphorylation	Uniprot
Y169	Phosphorylation	Uniprot
T175	Phosphorylation	Uniprot
S179	Phosphorylation	Uniprot
S183	Phosphorylation	Uniprot

Research Backgrounds

P02794_FRIH_HUMAN

Function:

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).

Tissue Specificity:

Expressed in the liver.

Subunit Structure:

Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. In the human liver, the heavy chain is predominant. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.

Family&Domains:

Belongs to the ferritin family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

Restrictive clause

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Ferritin Heavy Chain Antibody - #DF7705