ARPC5 Antibody - #DF7699

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Product: ARPC5 Antibody
Catalog: DF7699
Description: Rabbit polyclonal antibody to ARPC5
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Horse, Chicken
Mol.Wt.: 16 kDa; 16kD(Calculated).
Uniprot: O15511
RRID: AB_2841170

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ARPC5 Antibody detects endogenous levels of total ARPC5.
RRID:
AB_2841170
Cite Format: Affinity Biosciences Cat# DF7699, RRID:AB_2841170.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Actin related protein 2/3 complex subunit 5 (16 kD); Actin related protein 2/3 complex subunit 5; Actin related protein 2/3 complex, subunit 5 16kDa; Actin-related protein 2/3 complex subunit 5; ARC16; Arp2/3 complex 16 kDa subunit; Arp2/3 protein complex subunit p16; ARPC 5; Arpc5; ARPC5_HUMAN; dJ127C7.3; MGC88523; p16 Arc; p16-ARC; RP1 127C7.3;

Immunogens

Immunogen:
Uniprot:

O15511(ARPC5_HUMAN) >>Visit HPA database.

Gene(ID):
ARPC5(10092)
Sequence:
MSKNTVSSARFRKVDVDEYDENKFVDEEDGGDGQAGPDEGEVDSCLRQGNMTAALQAALKNPPINTKSQAVKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAMLLQWHEKALAAGGVGSIVRVLTARKTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Chicken
100
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15511 As Substrate

Site PTM Type Enzyme
S2 Acetylation
K3 Sumoylation
K3 Ubiquitination
K13 Ubiquitination
Y19 Phosphorylation
K23 Sumoylation
K23 Ubiquitination
S44 Phosphorylation
K60 Ubiquitination
K67 Ubiquitination
R74 Methylation
S77 Phosphorylation P49137 (MAPKAPK2)
K87 Ubiquitination
K93 Ubiquitination
K100 Ubiquitination
K108 Ubiquitination
K112 Ubiquitination
K131 Ubiquitination
S140 Phosphorylation
T146 Phosphorylation

Research Backgrounds

Function:

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

PTMs:

Polyubiquitinated by RNF128 with 'Lys-63'-linked chains, leading to proteasomal degradation.

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell projection. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.

Family&Domains:

Belongs to the ARPC5 family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

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