IRS4 Antibody - #DF7698

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Product: IRS4 Antibody
Catalog: DF7698
Description: Rabbit polyclonal antibody to IRS4
Application: WB IHC
Reactivity: Human
Prediction: Bovine, Horse, Rabbit
Mol.Wt.: 133 kDa; 134kD(Calculated).
Uniprot: O14654
RRID: AB_2841169

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(91%), Horse(80%), Rabbit(83%)
Clonality:
Polyclonal
Specificity:
IRS4 Antibody detects endogenous levels of total IRS4.
RRID:
AB_2841169
Cite Format: Affinity Biosciences Cat# DF7698, RRID:AB_2841169.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

160 kDa phosphotyrosine protein; Insulin receptor substrate 4; IRS 4; PY160;

Immunogens

Immunogen:
Uniprot:

O14654(IRS4_HUMAN) >>Visit HPA database.

Gene(ID):
IRS4(8471)
Expression:
O14654 IRS4_HUMAN:

Expressed in myoblasts. Expressed in liver and hepatocellular carcinoma.

Sequence:
MASCSFTRDQATRRLRGAAAAAAAALAAVVTTPLLSSGTPTALIGTGSSCPGAMWLSTATGSRSDSESEEEDLPVGEEVCKRGYLRKQKHGHRRYFVLKLETADAPARLEYYENARKFRHSVRAAAAAAAAAASGAAIPPLIPPRRVITLYQCFSVSQRADARYRHLIALFTQDEYFAMVAENESEQESWYLLLSRLILESKRRRCGTLGAQPDGEPAALAAAAAAEPPFYKDVWQVIVKPRGLGHRKELSGVFRLCLTDEEVVFVRLNTEVASVVVQLLSIRRCGHSEQYFFLEVGRSTVIGPGELWMQVDDCVVAQNMHELFLEKMRALCADEYRARCRSYSISIGAHLLTLLSARRHLGLVPLEPGGWLRRSRFEQFCHLRAIGDGEDEMLFTRRFVTPSEPVAHSRRGRLHLPRGRRSRRAVSVPASFFRRLAPSPARPRHPAEAPNNGARLSSEVSGSGSGNFGEEGNPQGKEDQEGSGGDYMPMNNWGSGNGRGSGGGQGSNGQGSSSHSSGGNQCSGEGQGSRGGQGSNGQGSGGNQCSRDGQGTAGGHGSGGGQRPGGGHGSGGGQGPGDGHGSGGGKNSGGGKGSGSGKGSDGDGERGKSLKKRSYFGKLTQSKQQQMPPPPPPPPPPPPAGGTGGKGKSGGRFRLYFCVDRGATKECKEAKEVKDAEIPEGAARGPHRARAFDEDEDDPYVPMRPGVATPLVSSSDYMPMAPQNVSASKKRHSRSPFEDSRGYMMMFPRVSPPPAPSPPKAPDTNKEDDSKDNDSESDYMFMAPGAGAIPKNPRNPQGGSSSKSWSSYFSLPNPFRSSPLGQNDNSEYVPMLPGKFLGRGLDKEVSYNWDPKDAASKPSGEGSFSKPGDGGSPSKPSDHEPPKNKAKRPNRLSFITKGYKIKPKPQKPTHEQREADSSSDYVNMDFTKRESNTPAPSTQGLPDSWGIIAEPRQSAFSNYVNVEFGVPFPNPANDLSDLLRAIPRANPLSLDSARWPLPPLPLSATGSNAIEEEGDYIEVIFNSAMTPAMALADSAIRYDAETGRIYVVDPFSECCMDISLSPSRCSEPPPVARLLQEEEQERRRPQSRSQSFFAAARAAVSAFPTDSLERDLSPSSAPAVASAAEPTLALSQVVAAASALAAAPGIGAAAAAAGFDSASARWFQPVANAADAEAVRGAQDVAGGSNPGAHNPSANLARGDNQAGGAAAAAAAPEPPPRSRRVPRPPEREDSDNDDDTHVRMDFARRDNQFDSPKRGR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
91
Rabbit
83
Horse
80
Pig
67
Chicken
67
Dog
56
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14654 As Substrate

Site PTM Type Enzyme
Phosphorylation
T7 Phosphorylation
S64 Phosphorylation
S66 Phosphorylation
S68 Phosphorylation
K99 Ubiquitination
Y111 Phosphorylation
K202 Ubiquitination
T208 Phosphorylation
K240 Ubiquitination
K248 Ubiquitination
S251 Phosphorylation
Y291 Phosphorylation
S375 Phosphorylation
R384 Methylation
R398 Methylation
T401 Phosphorylation
S403 Phosphorylation
S409 Phosphorylation
R410 Methylation
S427 Phosphorylation
S439 Phosphorylation
R442 Methylation
R444 Methylation
S458 Phosphorylation
S501 Phosphorylation
S517 Phosphorylation
S523 Phosphorylation
T552 Phosphorylation
S558 Phosphorylation
S570 Phosphorylation
S588 Phosphorylation
K618 Ubiquitination
T620 Phosphorylation
S622 Phosphorylation
R661 Methylation
K671 Acetylation
K674 Ubiquitination
R684 Methylation
Y700 Phosphorylation
S714 Phosphorylation
S715 Phosphorylation
Y717 Phosphorylation
S728 Phosphorylation
R741 Methylation
Y743 Phosphorylation
R749 Methylation
S751 Phosphorylation
S757 Phosphorylation
S775 Phosphorylation
S777 Phosphorylation
Y779 Phosphorylation
K803 Ubiquitination
S804 Phosphorylation
Y808 Phosphorylation
S810 Phosphorylation
S817 Phosphorylation
S826 Phosphorylation
Y828 Phosphorylation
K835 Ubiquitination
R839 Methylation
K843 Ubiquitination
S856 Phosphorylation
S859 Phosphorylation
S863 Phosphorylation
S865 Phosphorylation
S872 Phosphorylation
S874 Phosphorylation
S877 Phosphorylation
K897 Ubiquitination
R913 Methylation
S919 Phosphorylation
Y921 Phosphorylation
K928 Ubiquitination
T938 Phosphorylation
R952 Methylation
Y1038 Phosphorylation
Y1046 Phosphorylation
S1061 Phosphorylation
S1091 Phosphorylation
R1097 Methylation
S1107 Phosphorylation
R1176 Methylation
S1231 Phosphorylation
T1237 Phosphorylation
S1252 Phosphorylation
K1254 Ubiquitination

Research Backgrounds

Function:

Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2.

PTMs:

Phosphorylated on tyrosine residues in response to both insulin and IGF1 signaling. Phosphorylated on Tyr-921 in response to FGF2 signaling. Phosphorylation of Tyr-921 is required for GRB2, phospholipase C-gamma and phosphatidylinositol 3-kinase interaction.

Subcellular Location:

Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in myoblasts. Expressed in liver and hepatocellular carcinoma.

Subunit Structure:

Interacts with SOCS6 in response to stimulation with either insulin or IGF1 (By similarity). Interacts with CRK and CRKL. Interaction with CRK is stronger than with CRKL. Interacts with CRK via the phosphorylated YXXM motifs. Interacts with GRB2 and PIK3R1. Interacts with PLC-gamma, SHC1, PTK6, PPP4C and NISCH.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

Restrictive clause

 

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