LDLR Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from HepG2 cells, using LDLR Antibody.

DF7696 at 1/100 staining Rat kidney tissue by IHC-P.

Product:	LDLR Antibody
Catalog:	DF7696
Description:	Rabbit polyclonal antibody to LDLR
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	140 kDa; 95kD(Calculated).
Uniprot:	P01130
RRID:	AB_2841167

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(83%), Horse(100%), Sheep(92%), Rabbit(83%), Dog(92%)

Clonality:

Polyclonal

Specificity:

LDLR Antibody detects endogenous levels of total LDLR.

RRID:

AB_2841167
Cite Format: Affinity Biosciences Cat# DF7696, RRID:AB_2841167.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FH; FHC; LDL R; LDL receptor; LDLCQ2; Ldlr; LDLR_HUMAN; Low Density Lipoprotein Receptor; Low density lipoprotein receptor class A domain containing protein 3; Low density lipoprotein receptor familial hypercholesterolemia; Low-density lipoprotein receptor;

Immunogens

Immunogen:

Uniprot:

P01130(LDLR_HUMAN) >>Visit HPA database.

Gene(ID):

LDLR(3949)

Sequence:

P01130 LDLR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGPWGWKLRWTVALLLAAAGTAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAGRGNEKKPSSVRALSIVLPIVLLVFLCLGVFLLWKNWRLKNINSINFDNPVYQKTTEDEVHICHNQDGYSYPSRQMVSLEDDVA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

Dog

Bovine

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01130 As Substrate

P01130

Site	PTM Type	Source
K38	Ubiquitination	Uniprot
S110	Phosphorylation	Uniprot
N156	N-Glycosylation	Uniprot
N272	N-Glycosylation	Uniprot
K390	Ubiquitination	Uniprot
Y465	Phosphorylation	Uniprot
S470	Phosphorylation	Uniprot
S517	Phosphorylation	Uniprot
K596	Ubiquitination	Uniprot
K603	Ubiquitination	Uniprot
N657	N-Glycosylation	Uniprot
T713	Phosphorylation	Uniprot
T721	O-Glycosylation	Uniprot
T724	O-Glycosylation	Uniprot
S725	O-Glycosylation	Uniprot
T726	O-Glycosylation	Uniprot
K730	Ubiquitination	Uniprot
S732	O-Glycosylation	Uniprot
S733	O-Glycosylation	Uniprot
T734	O-Glycosylation	Uniprot
T738	O-Glycosylation	Uniprot
T741	O-Glycosylation	Uniprot
T742	O-Glycosylation	Uniprot
T743	O-Glycosylation	Uniprot
S750	O-Glycosylation	Uniprot
S820	Phosphorylation	Uniprot
Y828	Phosphorylation	Uniprot
K830	Ubiquitination	Uniprot
Y845	Phosphorylation	Uniprot
S846	Phosphorylation	Uniprot
Y847	Phosphorylation	Uniprot

Research Backgrounds

P01130_LDLR_HUMAN

Function:

Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.

(Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins.

(Microbial infection) Acts as a receptor for Vesicular stomatitis virus.

(Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.

PTMs:

N- and O-glycosylated.

Ubiquitinated by MYLIP leading to degradation.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Membrane>Clathrin-coated pit. Golgi apparatus. Early endosome. Late endosome. Lysosome.
Note: Rapidly endocytosed upon ligand binding.

Subunit Structure:

Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif (By similarity). Interacts (via NPXY motif) with LDLRAP1 (via PID domain). Interacts with ARRB1. Interacts with SNX17. Interacts with the full-length immature form of PCSK9 (via C-terminus).

(Microbial infection) Interacts with vesicular stomatitis virus glycoprotein.

(Microbial infection) May interact with HIV-1 Tat.

Family&Domains:

The NPXY motif mediates the interaction with the clathrin adapter DAB2 and with LDLRAP1 which are involved in receptor internalization. A few residues outside the motif also play a role in the interaction.

Belongs to the LDLR family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Cholesterol metabolism.

References

1). Sleeve Gastrectomy Suppresses Hepatic De Novo Cholesterogenesis and Improves Hepatic Cholesterol Accumulation in Obese Rats with Type 2 Diabetes Mellitus. NUTRITION, 2022 (PubMed: 34952362) [IF=4.4]

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LDLR Antibody - #DF7696