ABCG1 Antibody - #DF7693

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Product: ABCG1 Antibody
Catalog: DF7693
Description: Rabbit polyclonal antibody to ABCG1
Application: WB
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 110 kDa; 76kD(Calculated).
Uniprot: P45844
RRID: AB_2841164

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ABCG1 Antibody detects endogenous levels of total ABCG1.
RRID:
AB_2841164
Cite Format: Affinity Biosciences Cat# DF7693, RRID:AB_2841164.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABC transporter 8; ABC8; ABCG1; ABCG1_HUMAN; ATP-binding cassette sub family G member 1; ATP-binding cassette sub-family G member 1; ATP-binding cassette transporter 8; ATP-binding cassette transporter member 1 of subfamily G; ATP-binding cassette, sub family G WHITE member 1; homolog of Drosophila white; MGC34313; White protein homolog; White protein homolog ATP binding cassette transporter 8; WHITE1; WHT1;

Immunogens

Immunogen:
Uniprot:

P45844(ABCG1_HUMAN) >>Visit HPA database.

Gene(ID):
ABCG1(9619)
Expression:
P45844 ABCG1_HUMAN:

Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.

Sequence:
MACLMAAFSVGTAMNASSYSAEMTEPKSVCVSVDEVVSSNMEATETDLLNGHLKKVDNNLTEAQRFSSLPRRAAVNIEFRDLSYSVPEGPWWRKKGYKTLLKGISGKFNSGELVAIMGPSGAGKSTLMNILAGYRETGMKGAVLINGLPRDLRCFRKVSCYIMQDDMLLPHLTVQEAMMVSAHLKLQEKDEGRREMVKEILTALGLLSCANTRTGSLSGGQRKRLAIALELVNNPPVMFFDEPTSGLDSASCFQVVSLMKGLAQGGRSIICTIHQPSAKLFELFDQLYVLSQGQCVYRGKVCNLVPYLRDLGLNCPTYHNPADFVMEVASGEYGDQNSRLVRAVREGMCDSDHKRDLGGDAEVNPFLWHRPSEEVKQTKRLKGLRKDSSSMEGCHSFSASCLTQFCILFKRTFLSIMRDSVLTHLRITSHIGIGLLIGLLYLGIGNEAKKVLSNSGFLFFSMLFLMFAALMPTVLTFPLEMGVFLREHLNYWYSLKAYYLAKTMADVPFQIMFPVAYCSIVYWMTSQPSDAVRFVLFAALGTMTSLVAQSLGLLIGAASTSLQVATFVGPVTAIPVLLFSGFFVSFDTIPTYLQWMSYISYVRYGFEGVILSIYGLDREDLHCDIDETCHFQKSEAILRELDVENAKLYLDFIVLGIFFISLRLIAYFVLRYKIRAER

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Zebrafish
83
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P45844 As Substrate

Site PTM Type Enzyme
K54 Ubiquitination
K55 Ubiquitination
K98 Ubiquitination
T99 Phosphorylation
K102 Ubiquitination
S105 Phosphorylation
K107 Ubiquitination
K140 Ubiquitination
K189 Ubiquitination
K300 Ubiquitination
K354 Ubiquitination
K376 Ubiquitination

Research Backgrounds

Function:

Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrlysis of ATP. The lipid efflux is ALB-dependent. Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol.

PTMs:

Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cell membrane.
Note: Predominantly localized in the intracellular compartments mainly associated with the endoplasmic reticulum (ER) and Golgi membranes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.

Subunit Structure:

Homodimer; disulfide-linked. Homooligomer. May form heterodimers with several heterologous partners of the ABCG subfamily. Interacts with CAV1; this interaction regulates ABCG1-mediated cholesterol efflux.

Family&Domains:

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

Research Fields

· Environmental Information Processing > Membrane transport > ABC transporters.

References

1). Guang Chen Pi (the pericarp of Citrus reticulata Blanco's cultivars 'Chachi') inhibits macrophage-derived foam cell formation. JOURNAL OF ETHNOPHARMACOLOGY, 2022 (PubMed: 35489660) [IF=5.4]
2). Three polymethoxyflavones from the peel of Citrus reticulata “Chachi” inhibits oxidized low-density lipoprotein-induced macrophage-derived foam cell formation. Frontiers in Cardiovascular Medicine, 2022 (PubMed: 35966555) [IF=3.6]

Application: WB    Species: Mice    Sample: RAW264.7 cells

FIGURE 6 Effects of TAN, HxMF, and HpMF on ox-LDL-induced cholesterol efflux (A); effects of TAN, HxMF, and HpMF on mRNA expression of SRB1 and ABCG1 in ox-LDL-treated RAW264.7 cells (B,C); effects of TAN, HxMF, and HpMF on protein expression of SRB1 in ox-LDL-treated RAW264.7 cells (D–F); and effects of TAN, HxMF, and HpMF on protein expression of ABCG1 in ox-LDL-treated RAW264.7 cells (G–I). All experiments were run in triplicate and data showed mean ± SD. ##p < 0.01 compared with the control group, *p < 0.05 and **p < 0.01 compared with the ox-LDL group.

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