MMP12 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/3

Western blot analysis of extracts from COS-7, using MMP12 Antibody.

Figure 5 Ac-SDKP inhibits TLR4 and RANKL signaling in NR8383 cells treated with SiO2.

Product:	MMP12 Antibody
Catalog:	DF7686
Description:	Rabbit polyclonal antibody to MMP12
Application:	WB IF/ICC
Reactivity:	Human, Monkey
Prediction:	Rabbit
Mol.Wt.:	54 kDa; 54kD(Calculated).
Uniprot:	P39900
RRID:	AB_2841157

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Monkey

Prediction:

Rabbit(82%)

Clonality:

Polyclonal

Specificity:

MMP12 Antibody detects endogenous levels of total MMP12.

RRID:

AB_2841157
Cite Format: Affinity Biosciences Cat# DF7686, RRID:AB_2841157.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EC 3.4.24.65; HME; Macrophage elastase; Macrophage metalloelastase; Macrophage metaloelastase; Matrix metallopeptidase 12 (macrophage elastase); Matrix metalloprotease 12; Matrix metalloproteinase-12; ME; MGC138506; MME; MMP 12; MMP-12; Mmp12; MMP12_HUMAN;

Immunogens

Immunogen:

Uniprot:

P39900(MMP12_HUMAN) >>Visit HPA database.

Gene(ID):

MMP12(4321)

Expression:

P39900 MMP12_HUMAN:

Found in alveolar macrophages but not in peripheral blood monocytes.

Sequence:

P39900 MMP12_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKFLLILLLQATASGALPLNSSTSLEKNNVLFGERYLEKFYGLEINKLPVTKMKYSGNLMKEKIQEMQHFLGLKVTGQLDTSTLEMMHAPRCGVPDVHHFREMPGGPVWRKHYITYRINNYTPDMNREDVDYAIRKAFQVWSNVTPLKFSKINTGMADILVVFARGAHGDFHAFDGKGGILAHAFGPGSGIGGDAHFDEDEFWTTHSGGTNLFLTAVHEIGHSLGLGHSSDPKAVMFPTYKYVDINTFRLSADDIRGIQSLYGDPKENQRLPNPDNSEPALCDPNLSFDAVTTVGNKIFFFKDRFFWLKVSERPKTSVNLISSLWPTLPSGIEAAYEIEARNQVFLFKDDKYWLISNLRPEPNYPKSIHSFGFPNFVKKIDAAVFNPRFYRTYFFVDNQYWRYDERRQMMDPGYPKLITKNFQGIGPKIDAVFYSKNKYYYFFQGSNQFEYDFLLQRITKTLKSNSWFGC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Sheep

Horse

Dog

Pig

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P39900 As Substrate

P39900

Site	PTM Type	Source
Y113	Phosphorylation	Uniprot
Y116	Phosphorylation	Uniprot
Y121	Phosphorylation	Uniprot
Y414	Phosphorylation	Uniprot
Y434	Phosphorylation	Uniprot

Research Backgrounds

P39900_MMP12_HUMAN

Function:

May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Tissue Specificity:

Found in alveolar macrophages but not in peripheral blood monocytes.

Family&Domains:

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Belongs to the peptidase M10A family.

References

1). Ac-SDKP Attenuates Activation of Lung Macrophages and Bone Osteoclasts in Rats Exposed to Silica by Inhibition of TLR4 and RANKL Signaling Pathways. Journal of Inflammation Research, 2023 (PubMed: 33948088) [IF=4.5]

Application: WB Species: Mouse Sample: NR8383 cells

Figure 5 Ac-SDKP inhibits TLR4 and RANKL signaling in NR8383 cells treated with SiO2. (A) TRAP staining, Bar= 50 μm; (B) IF staining of TLR4, Bar=100 μm; (C) Protein expression of RANKL, RANK, OC-STAMP, AP-1, NFATc1, TRAF6, NF-κB, TLR4, MyD88, p-IκBα, TNF-α, and MMP-12 in NR8383 cells treated with SiO2 and Ac-SDKP or not. Data are presented as the mean ± SD. n = 3 per group.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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MMP12 Antibody - #DF7686