DNAJC3 Antibody - #DF7685
| Product: | DNAJC3 Antibody |
| Catalog: | DF7685 |
| Description: | Rabbit polyclonal antibody to DNAJC3 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| Mol.Wt.: | 54 kDa; 58kD(Calculated). |
| Uniprot: | Q13217 |
| RRID: | AB_2841156 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF7685, RRID:AB_2841156.
Fold/Unfold
DnaJ (Hsp40) homolog subfamily C member 3; DnaJ homolog subfamily C member 3; DNAJC3; DNJC3_HUMAN; double-stranded RNA-activated protein kinase inhibitor; Endoplasmic reticulum DnaJ protein 6; ERdj6; HP58; Interferon induced, double stranded RNA activated protein kinase inhibitor; Interferon-induced; P58; p58 ipk; P58IPK; PRKRI; Protein kinase inhibitor of 58 kDa; Protein kinase inhibitor p58; Protein kinase interferon inducible double stranded RNA dependent inhibitor;
Immunogens
Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
- Q13217 DNJC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVAPGSVTSRLGSVFPFLLVLVDLQYEGAECGVNADVEKHLELGKKLLAAGQLADALSQFHAAVDGDPDNYIAYYRRATVFLAMGKSKAALPDLTKVIQLKMDFTAARLQRGHLLLKQGKLDEAEDDFKKVLKSNPSENEEKEAQSQLIKSDEMQRLRSQALNAFGSGDYTAAIAFLDKILEVCVWDAELRELRAECFIKEGEPRKAISDLKAASKLKNDNTEAFYKISTLYYQLGDHELSLSEVRECLKLDQDHKRCFAHYKQVKKLNKLIESAEELIRDGRYTDATSKYESVMKTEPSIAEYTVRSKERICHCFSKDEKPVEAIRVCSEVLQMEPDNVNALKDRAEAYLIEEMYDEAIQDYETAQEHNENDQQIREGLEKAQRLLKQSQKRDYYKILGVKRNAKKQEIIKAYRKLALQWHPDNFQNEEEKKKAEKKFIDIAAAKEVLSDPEMRKKFDDGEDPLDAESQQGGGGNPFHRSWNSWQGFNPFSSGGPFRFKFHFN
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13217 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| T8 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| K46 | Methylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K150 | Ubiquitination | Uniprot | |
| S159 | Phosphorylation | Uniprot | |
| Y170 | Phosphorylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| Y232 | Phosphorylation | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K290 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| Y304 | Phosphorylation | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| K344 | Ubiquitination | Uniprot | |
| K382 | Ubiquitination | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| K407 | Methylation | Uniprot | |
| K412 | Ubiquitination | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| K446 | Ubiquitination | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| K457 | Ubiquitination | Uniprot |
Research Backgrounds
Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (By similarity). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
Endoplasmic reticulum.
Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
Interacts with EIF2AK4/GCN2; this interaction occurs under endoplasmic reticulum (ER) stress, hypothermic and amino acid starving stress conditions and inhibits EIF2AK4/GCN2 kinase activity. Interacts with EIF2AK3 (By similarity). Interacts with EIF2AK2. Forms a trimeric complex with DNAJB1 and HSPA8. Interacts with THAP12.
The J domain mediates interaction with HSPA8.
Binding to misfolded proteins is mediated by a hydrophobic patch forming a large groove within the first two TPR repeats.
Research Fields
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
· Human Diseases > Infectious diseases: Viral > Influenza A.
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