EPS15L1 Antibody - #DF7681

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Product: EPS15L1 Antibody
Catalog: DF7681
Description: Rabbit polyclonal antibody to EPS15L1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 94 kDa,70kDa; 94kD(Calculated).
Uniprot: Q9UBC2
RRID: AB_2841152

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(86%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
EPS15L1 Antibody detects endogenous levels of total EPS15L1.
RRID:
AB_2841152
Cite Format: Affinity Biosciences Cat# DF7681, RRID:AB_2841152.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EP15R_HUMAN; epidermal growth factor receptor pathway substrate 15- ike 1; Epidermal growth factor receptor substrate 15 like 1; Epidermal growth factor receptor substrate 15-like 1; epidermal growth factor receptor substrate EPS15R; Eps15 related protein; Eps15-related protein; EPS15L1; Eps15R;

Immunogens

Immunogen:
Uniprot:

Q9UBC2(EP15R_HUMAN) >>Visit HPA database.

Gene(ID):
EPS15L1(58513)
Sequence:
MAAPLIPLSQQIPTGNSLYESYYKQVDPAYTGRVGASEAALFLKKSGLSDIILGKIWDLADPEGKGFLDKQGFYVALRLVACAQSGHEVTLSNLNLSMPPPKFHDTSSPLMVTPPSAEAHWAVRVEEKAKFDGIFESLLPINGLLSGDKVKPVLMNSKLPLDVLGRVWDLSDIDKDGHLDRDEFAVAMHLVYRALEKEPVPSALPPSLIPPSKRKKTVFPGAVPVLPASPPPKDSLRSTPSHGSVSSLNSTGSLSPKHSLKQTQPTVNWVVPVADKMRFDEIFLKTDLDLDGYVSGQEVKEIFMHSGLTQNLLAHIWALADTRQTGKLSKDQFALAMYFIQQKVSKGIDPPQVLSPDMVPPSERGTPGPDSSGSLGSGEFTGVKELDDISQEIAQLQREKYSLEQDIREKEEAIRQKTSEVQELQNDLDRETSSLQELEAQKQDAQDRLDEMDQQKAKLRDMLSDVRQKCQDETQMISSLKTQIQSQESDLKSQEDDLNRAKSELNRLQQEETQLEQSIQAGRVQLETIIKSLKSTQDEINQARSKLSQLHESRQEAHRSLEQYDQVLDGAHGASLTDLANLSEGVSLAERGSFGAMDDPFKNKALLFSNNTQELHPDPFQTEDPFKSDPFKGADPFKGDPFQNDPFAEQQTTSTDPFGGDPFKESDPFRGSATDDFFKKQTKNDPFTSDPFTKNPSLPSKLDPFESSDPFSSSSVSSKGSDPFGTLDPFGSGSFNSAEGFADFSQMSKPPPSGPFTSSLGGAGFSDDPFKSKQDTPALPPKKPAPPRPKPPSGKSTPVSQLGSADFPEAPDPFQPLGADSGDPFQSKKGFGDPFSGKDPFVPSSAAKPSKASASGFADFTSVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Xenopus
92
Bovine
86
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBC2 As Substrate

Site PTM Type Enzyme
A2 Acetylation
Y30 Phosphorylation
S46 Phosphorylation
S49 Phosphorylation
Y74 Phosphorylation
T106 Phosphorylation
S107 Phosphorylation
S108 Phosphorylation
T113 Phosphorylation
S171 Phosphorylation
S229 Phosphorylation
S235 Phosphorylation
S238 Phosphorylation
T239 Phosphorylation
S241 Phosphorylation
S244 Phosphorylation
S246 Phosphorylation
S247 Phosphorylation
S250 Phosphorylation
T251 Phosphorylation
S253 Phosphorylation
S255 Phosphorylation
S259 Phosphorylation
K261 Ubiquitination
S295 Phosphorylation
S355 Phosphorylation
S362 Phosphorylation
T366 Phosphorylation
S371 Phosphorylation
S372 Phosphorylation
S374 Phosphorylation
S377 Phosphorylation
K384 Ubiquitination
S390 Phosphorylation
K400 Ubiquitination
S402 Phosphorylation
K417 Ubiquitination
T418 Phosphorylation
S434 Phosphorylation
S486 Phosphorylation
K492 Ubiquitination
T528 Phosphorylation
K531 Ubiquitination
K534 Ubiquitination
K546 Ubiquitination
S548 Phosphorylation
S553 Phosphorylation
S560 Phosphorylation
Y564 Phosphorylation
S575 Phosphorylation
T577 Phosphorylation
S583 Phosphorylation
S593 Phosphorylation
K602 Ubiquitination
K604 Ubiquitination
T612 Phosphorylation
K627 Ubiquitination
S628 Phosphorylation
K632 Ubiquitination
K638 Ubiquitination
K664 Ubiquitination
S666 Phosphorylation
S672 Phosphorylation
K679 Ubiquitination
K680 Ubiquitination
K683 Ubiquitination
S689 Phosphorylation
K694 Ubiquitination
S697 Phosphorylation
K701 Ubiquitination
S707 Phosphorylation
S708 Phosphorylation
S712 Phosphorylation
S714 Phosphorylation
S717 Phosphorylation
S718 Phosphorylation
K719 Ubiquitination
S734 Phosphorylation
T776 Phosphorylation
K782 Acetylation
K783 Acetylation
S793 Phosphorylation
T797 Phosphorylation
K828 Ubiquitination
K829 Ubiquitination
K838 Ubiquitination
K848 Ubiquitination
K851 Ubiquitination

Research Backgrounds

Function:

Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.

PTMs:

Phosphorylated on tyrosine residues by EGFR.

Subcellular Location:

Cell membrane>Peripheral membrane protein. Nucleus. Membrane>Coated pit.
Note: Localized to plasma membrane coated pits.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with EPS15, AGFG1/HRB and AGFG2/HRBL. Associates with the clathrin-associated adapter protein complex 2 (AP-2) (By similarity). Interacts with FCHO1. Interacts with FCHO2. Interacts (via EH domains) with DAB2. Interacts with UBQLN1 (via ubiquitin-like domain). Interacts with CAVIN3 (via leucine-zipper domain).

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

References

1). LncRNA ABHD11-AS1 promotes tumor progression in papillary thyroid carcinoma by regulating EPS15L1/EGFR signaling pathway. Clinical & Translational Oncology, 2022 (PubMed: 35098448) [IF=3.4]

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