NEFM Antibody - #DF7673

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Product: NEFM Antibody
Catalog: DF7673
Description: Rabbit polyclonal antibody to NEFM
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 150 KD; 102kD(Calculated).
Uniprot: P07197
RRID: AB_2841145

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
NEFM Antibody detects endogenous levels of total NEFM.
RRID:
AB_2841145
Cite Format: Affinity Biosciences Cat# DF7673, RRID:AB_2841145.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

150kDa medium; NEF3; NEFM; Neurofilament 3; NF160; NFM;

Immunogens

Immunogen:
Uniprot:

P07197(NFM_HUMAN) >>Visit HPA database.

Gene(ID):
NEFM(4741)
Sequence:
MSYTLDSLGNPSAYRRVTETRSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSMLAPRLAYSSAMLSSAESSLDFSQSSSLLNGGSGPGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEEASLVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLESHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRPPITISSKIQKPKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSMKEEKKEAAEEKEEEPEAEEEEVAAKKSPVKATAPEVKEEEGEKEEEEGQEEEEEEDEGAKSDQAEEGGSEKEGSSEKEEGEQEEGETEAEAEGEEAEAKEEKKVEEKSEEVATKEELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGEQKEEEEKEVKEAPKEEKVEKKEEKPKDVPEKKKAESPVKEEAVAEVVTITKSVKVHLEKETKEEGKPLQQEKEKEKAGGEGGSEEEGSDKGAKGSRKEDIAVNGEVEGKEEVEQETKEKGSGREEEKGVVTNGLDLSPADEKKGGDKSEEKVVVTKTVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07197 As Substrate

Site PTM Type Enzyme
R21 Methylation
S23 Phosphorylation
R26 Methylation
S28 Phosphorylation
S30 Phosphorylation
S33 Phosphorylation
S37 Phosphorylation
R42 Methylation
S44 Phosphorylation
R54 Methylation
S55 Phosphorylation
S73 Phosphorylation
R111 Methylation
K118 Ubiquitination
S226 Phosphorylation
K259 Ubiquitination
K263 Ubiquitination
K271 Ubiquitination
S333 Phosphorylation
R382 Methylation
Y384 Phosphorylation
Y401 Phosphorylation
K445 Ubiquitination
K451 Ubiquitination
S467 Phosphorylation
S511 Phosphorylation
S545 Phosphorylation
S553 Phosphorylation
S558 Phosphorylation
S559 Phosphorylation
S615 Phosphorylation
S620 Phosphorylation
S628 Phosphorylation
S633 Phosphorylation
S641 Phosphorylation
S646 Phosphorylation
K653 Acetylation
S654 Phosphorylation
S659 Phosphorylation
S667 Phosphorylation
S670 Phosphorylation
S672 Phosphorylation
S680 Phosphorylation
S685 Phosphorylation
K693 Acetylation
K698 Acetylation
S736 Phosphorylation
T748 Phosphorylation
T750 Phosphorylation
S783 Phosphorylation
S788 Phosphorylation
S821 Phosphorylation
S837 Phosphorylation
K871 Ubiquitination
Y872 Phosphorylation
K875 Ubiquitination

Research Backgrounds

Function:

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

PTMs:

There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.

Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.

Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

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