Product: MEK5 Antibody
Catalog: DF7667
Description: Rabbit polyclonal antibody to MEK5
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 50kDa; 50kD(Calculated).
Uniprot: Q13163
RRID: AB_2841140

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MEK5 Antibody detects endogenous levels of total MEK5.
RRID:
AB_2841140
Cite Format: Affinity Biosciences Cat# DF7667, RRID:AB_2841140.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dual specificity mitogen activated protein kinase kinase 5; Dual specificity mitogen-activated protein kinase kinase 5; EC 2.7.12.2; HsT17454; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAP2K5; MAPK/ERK kinase 5; MAPKK 5; MAPKK5; MEK 5; mitogen-activated protein kinase kinase 5; MKK5; MP2K5_HUMAN; PRKMK5; Protein kinase, mitogen-activated, kinase 5; SAPKK5; SKK5;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q13163 MP2K5_HUMAN:

Expressed in many adult tissues. Abundant in heart and skeletal muscle.

Sequence:
MLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHSSPAVSDSLPSNSLKKSSAELKKILANGQMNEQDIRYRDTLGHGNGGTVYKAYHVPSGKILAVKVILLDITLELQKQIMSELEILYKCDSSYIIGFYGAFFVENRISICTEFMDGGSLDVYRKMPEHVLGRIAVAVVKGLTYLWSLKILHRDVKPSNMLVNTRGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIHSDVWSLGISFMELALGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPVGEFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNAAVVSMWVCRALEERRSQQGPP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13163 As Substrate

Site PTM Type Enzyme
T71 Phosphorylation
K121 Ubiquitination
R125 Methylation
S129 Phosphorylation Q13164 (MAPK7)
S133 Phosphorylation
S137 Phosphorylation Q13164 (MAPK7)
S142 Phosphorylation Q13164 (MAPK7)
K146 Ubiquitination
S148 Phosphorylation
S149 Phosphorylation Q13164 (MAPK7)
K154 Ubiquitination
K298 Ubiquitination
S311 Phosphorylation
T315 Phosphorylation

PTMs - Q13163 As Enzyme

Substrate Site Source
Q13164 (MAPK7) T219 Uniprot
Q13164 (MAPK7) Y221 Uniprot

Research Backgrounds

Function:

Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.

PTMs:

Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.

Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.

Tissue Specificity:

Expressed in many adult tissues. Abundant in heart and skeletal muscle.

Subunit Structure:

Interacts with PARD6A, MAP3K3 and MAPK7. Forms a complex with SQSTM1 and PRKCZ or PRKCI (By similarity). Interacts with Yersinia yopJ.

Family&Domains:

Binds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the PB1 domain. This domain also mediates interactions with SQSTM1 and PARD6A (By similarity).

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

References

1). iTRAQ based proteomic analysis of PM2.5 induced lung damage. RSC Advances, 2019 (PubMed: 35517034) [IF=3.9]

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