FCGR2B/CD32B Antibody - #DF7666

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Product: FCGR2B/CD32B Antibody
Catalog: DF7666
Description: Rabbit polyclonal antibody to FCGR2B/CD32B
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 44 kDa,30kDa; 34kD(Calculated).
Uniprot: P31994
RRID: AB_2841139

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
FCGR2B/CD32B Antibody detects endogenous levels of total FCGR2B/CD32B.
RRID:
AB_2841139
Cite Format: Affinity Biosciences Cat# DF7666, RRID:AB_2841139.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD32; CD32 antigen; CD32B; CDw32; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor for; Fc gamma receptor IIB; Fc gamma RII; Fc gamma RIIB; Fc-gamma RII; Fc-gamma RII-b; Fc-gamma-RIIb; FCG2; FCG2B_HUMAN; Fcgr2; FCGR2B; FCRII; FcRII-b; IGFR2; IgG Fc receptor II beta; IgG Fc receptor II-b; Low affinity immunoglobulin gamma Fc region receptor II; Low affinity immunoglobulin gamma Fc region receptor II-b; Ly-17; Lym 1; Lymphocyte antigen 17;

Immunogens

Immunogen:
Uniprot:

P31994(FCG2B_HUMAN) >>Visit HPA database.

Gene(ID):
FCGR2B(2213)
Expression:
P31994 FCG2B_HUMAN:

Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.

Sequence:
MGILSFLPVLATESDWADCKSPQPWGHMLLWTAVLFLAPVAGTPAAPPKAVLKLEPQWINVLQEDSVTLTCRGTHSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDSGEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIVLRCHSWKDKPLVKVTFFQNGKSKKFSRSDPNFSIPQANHSHSGDYHCTGNIGYTLYSSKPVTITVQAPSSSPMGIIVAVVTGIAVAAIVAAVVALIYCRKKRISALPGYPECREMGETLPEKPANPTNPDEADKVGAENTITYSLLMHPDALEEPDDQNRI

PTMs - P31994 As Substrate

Site PTM Type Enzyme
Y292 Phosphorylation P06241 (FYN) , P07948 (LYN) , P51451 (BLK)
S293 Phosphorylation

Research Backgrounds

Function:

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

PTMs:

Phosphorylated by the SRC-type Tyr-kinases LYN and BLK.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.

Subunit Structure:

Interacts with INPP5D/SHIP1. Interacts with FGR. Interacts with LYN.

(Microbial infection) Isoform IIB1 interacts with measles virus protein N. Protein N is released in the blood following lysis of measles infected cells. This interaction presumably block inflammatory immune response.

Family&Domains:

Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

References

1). Pentraxin 3 regulated by miR-224-5p modulates macrophage reprogramming and exacerbates osteoarthritis associated synovitis by targeting CD32. Cell Death & Disease, 2022 (PubMed: 35739102) [IF=9.0]

Application: IF/ICC    Species: Mouse    Sample: RAW264.7 cells

Fig. 5 PTX3 promotes OA by interaction with macrophage CD32. A, B Immunofluorescent staining and quantification of CD32 for RAW264.7 and chondrocytes, n = 5 per group. Scale bar: 50 µm. C, D Immunofluorescent staining and quantification of CD32 of synovial tissues (upper) and knee cartilage (lower) from controls and 8 week-DMM-OA mice, n = 5 per group. Scale bar: 50 µm. E Relative mRNA expression levels of IL-1β, IL-6 and TNF-α in Si-CD32- or rmPTX3-treated RAW264.7 cells, n = 9 per group. F Relative mRNA expression levels of iNOS and ARG in Si-CD32- or rmPTX3-treated RAW264.7 cells, n = 9 per group. G Immunoblotting of P-p65 and p65 of RAW264.7 cells treated with Si-CD32 or rmPTX3. H, I IF staining and quantification of F4/80 and P-p65 in RAW264.7 treated with Si-CD32 or rmPTX3, n = 6 per group. Scale bar: 25 µm. *P 

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