Caspase 5 Antibody - #DF7664

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Product: Caspase 5 Antibody
Catalog: DF7664
Description: Rabbit polyclonal antibody to Caspase 5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 47 kDa; 50kD(Calculated).
Uniprot: P51878
RRID: AB_2841138

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Caspase 5 Antibody detects endogenous levels of total Caspase 5.
RRID:
AB_2841138
Cite Format: Affinity Biosciences Cat# DF7664, RRID:AB_2841138.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Apoptosis related cysteine protease; CASP-5; CASP5; CASP5_HUMAN; Caspase-5 subunit p10; ICE(rel)-III; ICERELIII; ICH 3; ICH 3 protease; Protease ICH-3; Protease TY; TY; TY protease;

Immunogens

Immunogen:
Uniprot:

P51878(CASP5_HUMAN) >>Visit HPA database.

Gene(ID):
CASP5(838)
Expression:
P51878 CASP5_HUMAN:

Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.

Sequence:
MAEDSGKKKRRKNFEAMFKGILQSGLDNFVINHMLKNNVAGQTSIQTLVPNTDQKSTSVKKDNHKKKTVKMLEYLGKDVLHGVFNYLAKHDVLTLKEEEKKKYYDTKIEDKALILVDSLRKNRVAHQMFTQTLLNMDQKITSVKPLLQIEAGPPESAESTNILKLCPREEFLRLCKKNHDEIYPIKKREDRRRLALIICNTKFDHLPARNGAHYDIVGMKRLLQGLGYTVVDEKNLTARDMESVLRAFAARPEHKSSDSTFLVLMSHGILEGICGTAHKKKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGEKHGELWVRDSPASLALISSQSSENLEADSVCKIHEEKDFIAFCSSTPHNVSWRDRTRGSIFITELITCFQKYSCCCHLMEIFRKVQKSFEVPQAKAQMPTIERATLTRDFYLFPGN

PTMs - P51878 As Substrate

Site PTM Type Enzyme
S44 Phosphorylation
Y74 Phosphorylation
Y103 Phosphorylation
Y104 Phosphorylation
Y228 Phosphorylation
T229 Phosphorylation
T237 Phosphorylation
K282 Ubiquitination

Research Backgrounds

Function:

Mediator of programmed cell death (apoptosis). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation.

PTMs:

The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

Tissue Specificity:

Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.

Subunit Structure:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits. Interacts with MEFV.Interacts with NOD2. Interacts with SERPINB1; this interaction regulates CASP5 activity.

Family&Domains:

Belongs to the peptidase C14A family.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

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For Research Use Only.
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