CYTH3 Antibody | Affinity Biosciences

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Western blot analysis of CYTH3 using COLO205 whole lysates.

DF7653 at 1/100 staining mouse liver tissue by IHC-P.

Product:	CYTH3 Antibody
Catalog:	DF7653
Description:	Rabbit polyclonal antibody to CYTH3
Application:	WB IHC
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Dog, Chicken
Mol.Wt.:	46 kDa; 46kD(Calculated).
Uniprot:	O43739
RRID:	AB_2841129

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

CYTH3 Antibody detects endogenous levels of total CYTH3.

RRID:

AB_2841129
Cite Format: Affinity Biosciences Cat# DF7653, RRID:AB_2841129.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CYTH3; ARF nucleotide-binding site opener 3; ARNO3; ARNO3 protein; CYH3_HUMAN; Cyth3; Cytohesin-3; General receptor of phosphoinositides 1; Grp1; PH; PH, SEC7 and coiled-coil domain-containing protein 3; Protein ARNO3; PSCD3; SEC7 and coiled-coil domain-containing protein 3; SEC7 homolog C; Sec7-3; Sec7c;

Immunogens

Immunogen:

Uniprot:

O43739(CYH3_HUMAN) >>Visit HPA database.

Gene(ID):

CYTH3(9265)

Expression:

O43739 CYH3_HUMAN:

Almost absent from liver, thymus and peripheral blood lymphocytes.

Sequence:

O43739 CYH3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDEDGGGEGGGVPEDLSLEEREELLDIRRRKKELIDDIERLKYEIAEVMTEIDNLTSVEESKTTQRNKQIAMGRKKFNMDPKKGIQFLIENDLLQSSPEDVAQFLYKGEGLNKTVIGDYLGERDEFNIKVLQAFVELHEFADLNLVQALRQFLWSFRLPGEAQKIDRMMEAFASRYCLCNPGVFQSTDTCYVLSFAIIMLNTSLHNHNVRDKPTAERFIAMNRGINEGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGGRVKTWKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDPRKPNCFELYNPSHKGQVIKACKTEADGRVVEGNHVVYRISAPSPEEKEEWMKSIKASISRDPFYDMLATRKRRIANKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Chicken

100

Sheep

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O43739 As Substrate

O43739

Site	PTM Type	Source
K32	Ubiquitination	Uniprot
K107	Ubiquitination	Uniprot
K164	Ubiquitination	Uniprot
K244	Ubiquitination	Uniprot
K249	Ubiquitination	Uniprot
K273	Ubiquitination	Uniprot
Y359	Phosphorylation	Uniprot
S362	Phosphorylation	Uniprot
S375	Phosphorylation	Uniprot
Y386	Phosphorylation	Uniprot
T391	Phosphorylation	Uniprot

Research Backgrounds

O43739_CYH3_HUMAN

Function:

Promotes guanine-nucleotide exchange on ARF1 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. Plays a role in the epithelial polarization (By similarity).

Subcellular Location:

Cytoplasm>Cytosol. Cell membrane>Peripheral membrane protein. Cell junction>Adherens junction. Cell junction>Tight junction.
Note: Translocates from the cytosol to membranes enriched in phosphatidylinositol 3,4,5-trisphosphate.

Tissue Specificity:

Almost absent from liver, thymus and peripheral blood lymphocytes.

Subunit Structure:

Interacts with GRASP (By similarity). Interacts with ARF6. Interacts with FRMD4A (By similarity). Interacts with FRMD4B (By similarity).

Family&Domains:

Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate.

Autoinhibited by its C-terminal basic region.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)

Restrictive clause

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CYTH3 Antibody - #DF7653