ARHGAP5 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

Western blot analysis of ARHGAP5 using HeLa whole lysates.

DF7649 at 1/100 staining Mouse heart tissue by IHC-P.

DF7649 at 1/100 staining Mouse liver tissue by IHC-P.

DF7649 staining Hela cells by IF/ICC.

Product:	ARHGAP5 Antibody
Catalog:	DF7649
Description:	Rabbit polyclonal antibody to ARHGAP5
Application:	WB IHC IF/ICC
Reactivity:	Human
Prediction:	Pig, Zebrafish, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	172 kDa; 172kD(Calculated).
Uniprot:	Q13017
RRID:	AB_2841126

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Zebrafish(91%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%)

Clonality:

Polyclonal

Specificity:

ARHGAP5 Antibody detects endogenous levels of total ARHGAP5.

RRID:

AB_2841126
Cite Format: Affinity Biosciences Cat# DF7649, RRID:AB_2841126.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ARHGAP 5; ARHGAP5; p190 B; p190 RhoGAP; Rho GTPase activating protein 5; RhoGAP 5; RhoGAP; RhoGAP5;

Immunogens

Immunogen:

Uniprot:

Q13017(RHG05_HUMAN) >>Visit HPA database.

Gene(ID):

ARHGAP5(394)

Expression:

Q13017 RHG05_HUMAN:

Detected in skin fibroblasts (at protein level) (PubMed:8537347).

Sequence:

Q13017 RHG05_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MMAKNKEPRPPSYTISIVGLSGTEKDKGNCGVGKSCLCNRFVRSKADEYYPEHTSVLSTIDFGGRVVNNDHFLYWGDIIQNSEDGVECKIHVIEQTEFIDDQTFLPHRSTNLQPYIKRAAASKLQSAEKLMYICTDQLGLEQDFEQKQMPEGKLNVDGFLLCIDVSQGCNRKFDDQLKFVNNLFVQLSKSKKPVIIAATKCDECVDHYLREVQAFASNKKNLLVVETSARFNVNIETCFTALVQMLDKTRSKPKIIPYLDAYKTQRQLVVTATDKFEKLVQTVRDYHATWKTVSNKLKNHPDYEEYINLEGTRKARNTFSKHIEQLKQEHIRKRREEYINTLPRAFNTLLPNLEEIEHLNWSEALKLMEKRADFQLCFVVLEKTPWDETDHIDKINDRRIPFDLLSTLEAEKVYQNHVQHLISEKRRVEMKEKFKKTLEKIQFISPGQPWEEVMCFVMEDEAYKYITEADSKEVYGRHQREIVEKAKEEFQEMLFEHSELFYDLDLNATPSSDKMSEIHTVLSEEPRYKALQKLAPDRESLLLKHIGFVYHPTKETCLSGQNCTDIKVEQLLASSLLQLDHGRLRLYHDSTNIDKVNLFILGKDGLAQELANEIRTQSTDDEYALDGKIYELDLRPVDAKSPYFLSQLWTAAFKPHGCFCVFNSIESLSFIGEFIGKIRTEASQIRKDKYMANLPFTLILANQRDSISKNLPILRHQGQQLANKLQCPFVDVPAGTYPRKFNETQIKQALRGVLESVKHNLDVVSPIPANKDLSEADLRIVMCAMCGDPFSVDLILSPFLDSHSCSAAQAGQNNSLMLDKIIGEKRRRIQITILSYHSSIGVRKDELVHGYILVYSAKRKASMGMLRAFLSEVQDTIPVQLVAVTDSQADFFENEAIKELMTEGEHIATEITAKFTALYSLSQYHRQTEVFTLFFSDVLEKKNMIENSYLSDNTRESTHQSEDVFLPSPRDCFPYNNYPDSDDDTEAPPPYSPIGDDVQLLPTPSDRSRYRLDLEGNEYPIHSTPNCHDHERNHKVPPPIKPKPVVPKTNVKKLDPNLLKTIEAGIGKNPRKQTSRVPLAHPEDMDPSDNYAEPIDTIFKQKGYSDEIYVVPDDSQNRIKIRNSFVNNTQGDEENGFSDRTSKSHGERRPSKYKYKSKTLFSKAKSYYRRTHSDASDDEAFTTSKTKRKGRHRGSEEDPLLSPVETWKGGIDNPAITSDQELDDKKMKKKTHKVKEDKKQKKKTKNFNPPTRRNWESNYFGMPLQDLVTAEKPIPLFVEKCVEFIEDTGLCTEGLYRVSGNKTDQDNIQKQFDQDHNINLVSMEVTVNAVAGALKAFFADLPDPLIPYSLHPELLEAAKIPDKTERLHALKEIVKKFHPVNYDVFRYVITHLNRVSQQHKINLMTADNLSICFWPTLMRPDFENREFLSTTKIHQSVVETFIQQCQFFFYNGEIVETTNIVAPPPPSNPGQLVEPMVPLQLPPPLQPQLIQPQLQTDPLGII

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Sheep

100

Dog

100

Rabbit

100

Zebrafish

Chicken

Horse

Bovine

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13017 As Substrate

Q13017

Site	PTM Type	Enzyme	Source
S12	Phosphorylation		Uniprot
S16	Phosphorylation		Uniprot
S21	Phosphorylation		Uniprot
T23	Phosphorylation		Uniprot
T199	Phosphorylation		Uniprot
T289	Phosphorylation		Uniprot
Y306	Phosphorylation	P06213 (INSR)	Uniprot
K314	Acetylation		Uniprot
K321	Acetylation		Uniprot
K327	Ubiquitination		Uniprot
K394	Ubiquitination		Uniprot
K533	Ubiquitination		Uniprot
S540	Phosphorylation		Uniprot
S590	Phosphorylation		Uniprot
T591	Phosphorylation		Uniprot
K724	Ubiquitination		Uniprot
S765	Phosphorylation		Uniprot
S862	Phosphorylation		Uniprot
S948	Phosphorylation		Uniprot
S951	Phosphorylation		Uniprot
S968	Phosphorylation		Uniprot
S981	Phosphorylation		Uniprot
Y1019	Phosphorylation		Uniprot
T1024	Phosphorylation		Uniprot
K1053	Ubiquitination		Uniprot
K1060	Ubiquitination		Uniprot
S1088	Phosphorylation		Uniprot
Y1091	Phosphorylation		Uniprot
K1102	Ubiquitination		Uniprot
Y1104	Phosphorylation		Uniprot
S1105	Phosphorylation		Uniprot
Y1109	Phosphorylation	P12931 (SRC) , Q13882 (PTK6)	Uniprot
S1115	Phosphorylation		Uniprot
S1124	Phosphorylation		Uniprot
T1129	Phosphorylation		Uniprot
S1138	Phosphorylation		Uniprot
T1159	Phosphorylation		Uniprot
T1171	Phosphorylation		Uniprot
S1173	Phosphorylation		Uniprot
S1176	Phosphorylation		Uniprot
S1184	Phosphorylation		Uniprot
S1195	Phosphorylation		Uniprot
S1202	Phosphorylation		Uniprot
T1206	Phosphorylation		Uniprot
T1217	Phosphorylation		Uniprot
S1218	Phosphorylation		Uniprot
S1396	Phosphorylation		Uniprot

Research Backgrounds

Q13017_RHG05_HUMAN

Function:

GTPase-activating protein for Rho family members.

Subcellular Location:

Cytoplasm. Cell membrane>Peripheral membrane protein.
Note: Also membrane-associated in a fibrillar pattern that colocalizes with the alpha5-beta1 integrin receptor (ITGA5/ITGB1) for fibronectin.

Tissue Specificity:

Detected in skin fibroblasts (at protein level).

Subunit Structure:

May interact with RASA1/p120GAP.

Family&Domains:

The pG1 pseudoGTPase domain does not bind GTP.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

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ARHGAP5 Antibody - #DF7649