STXBP4 Antibody - #DF7620

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Product: STXBP4 Antibody
Catalog: DF7620
Description: Rabbit polyclonal antibody to STXBP4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Monkey
Prediction: Rat, Pig, Horse, Dog
Mol.Wt.: 68 kDa; 62kD(Calculated).
Uniprot: Q6ZWJ1
RRID: AB_2841107

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Rat(%), Pig(92%), Horse(92%), Dog(83%)
Clonality:
Polyclonal
Specificity:
STXBP4 Antibody detects endogenous levels of total STXBP4.
RRID:
AB_2841107
Cite Format: Affinity Biosciences Cat# DF7620, RRID:AB_2841107.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ16496; MGC149829; MGC50337; OTTHUMP00000082667; OTTHUMP00000227400; STX4-interacting protein; STXB4_HUMAN; Stxbp4; Synip; Syntaxin 4 interacting protein; Syntaxin 4-interacting protein; Syntaxin binding protein 4; Syntaxin-binding protein 4;

Immunogens

Immunogen:
Uniprot:

Q6ZWJ1(STXB4_HUMAN) >>Visit HPA database.

Gene(ID):
STXBP4(252983)
Sequence:
MNKNTSTVVSPSLLEKDPAFQMITIAKETGLGLKVLGGINRNEGPLVYIQEIIPGGDCYKDGRLKPGDQLVSVNKESMIGVSFEEAKSIITGAKLRLESAWEIAFIRQKSDNIQPENLSCTSLIEASGEYGPQASTLSLFSSPPEILIPKTSSTPKTNNDILSSCEIKTGYNKTVQIPITSENSTVGLSNTDVASAWTENYGLQEKISLNPSVRFKAEKLEMALNYLGIQPTKEQHQALRQQVQADSKGTVSFGDFVQVARNLFCLQLDEVNVGAHEISNILDSQLLPCDSSEADEMERLKCERDDALKEVNTLKEKLLESDKQRKQLTEELQNVKQEAKAVVEETRALRSRIHLAEAAQRQAHGMEMDYEEVIRLLEAKITELKAQLADYSDQNKESVQDLKKRIMVLDCQLRKSEMARKTFEASTEKLLHFVEAIQEVFSDNSTPLSNLSERRAVLASQTSLTPLGRNGRSIPATLALESKELVKSVRALLDMDCLPYGWEEAYTADGIKYFINHVTQTTSWIHPVMSVLNLSRSEENEEDCSRELPNQKS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Dog
83
Sheep
75
Rabbit
75
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6ZWJ1 As Substrate

Site PTM Type Enzyme
T5 Phosphorylation
S6 Phosphorylation
S10 Phosphorylation
S12 Phosphorylation
K16 Ubiquitination
K27 Ubiquitination
K60 Ubiquitination
K65 Ubiquitination
S82 Phosphorylation
S99 Phosphorylation P31751 (AKT2)
T151 Phosphorylation
K156 Ubiquitination
T157 Phosphorylation
S163 Phosphorylation
S164 Phosphorylation
K168 Ubiquitination
S208 Phosphorylation
S212 Phosphorylation
K233 Ubiquitination
K248 Ubiquitination
S252 Phosphorylation
K323 Ubiquitination
K326 Ubiquitination
K380 Ubiquitination
K385 Ubiquitination
Y391 Phosphorylation
K396 Ubiquitination
K421 Ubiquitination
S445 Phosphorylation
T446 Phosphorylation
S460 Phosphorylation
S463 Phosphorylation
T465 Phosphorylation
K483 Ubiquitination
K487 Ubiquitination
S545 Phosphorylation

Research Backgrounds

Function:

Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose (By similarity).

PTMs:

Phosphorylated on Ser-99 by PKB/AKT2 after insulin treatment. Phosphorylation on Ser-99 abolishes the interaction with STX4A (By similarity).

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with STX4A.

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