NRP2 Antibody | Affinity Biosciences

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NRP2 Antibody - Western blot analysis of Neuropilin-2 using COLO205 whole lysates.

Product:	NRP2 Antibody
Catalog:	DF7604
Description:	Rabbit polyclonal antibody to NRP2
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	120 kDa; 105kD(Calculated).
Uniprot:	O60462
RRID:	AB_2841095

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(86%)

Clonality:

Polyclonal

Specificity:

NRP2 Antibody detects endogenous levels of total NRP2.

RRID:

AB_2841095
Cite Format: Affinity Biosciences Cat# DF7604, RRID:AB_2841095.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MGC126574; Neuropilin 2; Neuropilin-2; Neuropilin2; NP 2; NP2; NPN 2; NPN2; NRP 2; Nrp2; NRP2_HUMAN; PRO2714; Receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; Vascular endothelial growth factor 165 receptor 2; VEGF1265R2; VEGF165R2;

Immunogens

Immunogen:

Uniprot:

O60462(NRP2_HUMAN) >>Visit HPA database.

Gene(ID):

NRP2(8828)

Sequence:

O60462 NRP2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDMFPLTWVFLALYFSRHQVRGQPDPPCGGRLNSKDAGYITSPGYPQDYPSHQNCEWIVYAPEPNQKIVLNFNPHFEIEKHDCKYDFIEIRDGDSESADLLGKHCGNIAPPTIISSGSMLYIKFTSDYARQGAGFSLRYEIFKTGSEDCSKNFTSPNGTIESPGFPEKYPHNLDCTFTILAKPKMEIILQFLIFDLEHDPLQVGEGDCKYDWLDIWDGIPHVGPLIGKYCGTKTPSELRSSTGILSLTFHTDMAVAKDGFSARYYLVHQEPLENFQCNVPLGMESGRIANEQISASSTYSDGRWTPQQSRLHGDDNGWTPNLDSNKEYLQVDLRFLTMLTAIATQGAISRETQNGYYVKSYKLEVSTNGEDWMVYRHGKNHKVFQANNDATEVVLNKLHAPLLTRFVRIRPQTWHSGIALRLELFGCRVTDAPCSNMLGMLSGLIADSQISASSTQEYLWSPSAARLVSSRSGWFPRIPQAQPGEEWLQVDLGTPKTVKGVIIQGARGGDSITAVEARAFVRKFKVSYSLNGKDWEYIQDPRTQQPKLFEGNMHYDTPDIRRFDPIPAQYVRVYPERWSPAGIGMRLEVLGCDWTDSKPTVETLGPTVKSEETTTPYPTEEEATECGENCSFEDDKDLQLPSGFNCNFDFLEEPCGWMYDHAKWLRTTWASSSSPNDRTFPDDRNFLRLQSDSQREGQYARLISPPVHLPRSPVCMEFQYQATGGRGVALQVVREASQESKLLWVIREDQGGEWKHGRIILPSYDMEYQIVFEGVIGKGRSGEIAIDDIRISTDVPLENCMEPISAFAGENFKVDIPEIHEREGYEDEIDDEYEVDWSNSSSATSGSGAPSTDKEKSWLYTLDPILITIIAMSSLGVLLGATCAGLLLYCTCSYSGLSSRSCTTLENYNFELYDGLKHKVKMNHQKCCSEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60462 As Substrate

O60462

Site	PTM Type	Source
N152	N-Glycosylation	Uniprot
N157	N-Glycosylation	Uniprot
T319	Phosphorylation	Uniprot
T494	Phosphorylation	Uniprot
R507	Methylation	Uniprot
T595	O-Glycosylation	Uniprot
T600	O-Glycosylation	Uniprot
T603	O-Glycosylation	Uniprot
T607	O-Glycosylation	Uniprot
S672	O-Glycosylation	Uniprot
S781	Phosphorylation	Uniprot

Research Backgrounds

O60462_NRP2_HUMAN

Function:

High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF.

(Microbial infection) Acts as a receptor for human cytomegalovirus pentamer-dependent entry in epithelial and endothelial cells.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Secreted.

Subunit Structure:

Heterodimer with NRP1. Binds PLXNB1.

(Microbial infection) Interacts with human cytomegalovirus proteins gL, UL128, UL130 and UL131A.

Family&Domains:

The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding.

Belongs to the neuropilin family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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NRP2 Antibody - #DF7604