ADFP Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of extracts from mouse brain, using ADFP Antibody.

Figure 6 Effects of ICAE on the protein expression of PPARγ and ADRP in the liver of HFD-induced mice.

Product:	ADFP Antibody
Catalog:	DF7603
Description:	Rabbit polyclonal antibody to ADFP
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	48 kDa; 48kD(Calculated).
Uniprot:	Q99541
RRID:	AB_2841094

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(83%), Rabbit(92%), Dog(100%)

Clonality:

Polyclonal

Specificity:

ADFP Antibody detects endogenous levels of total ADFP.

RRID:

AB_2841094
Cite Format: Affinity Biosciences Cat# DF7603, RRID:AB_2841094.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ADFP; Adipophilin; Adipose differentiation related protein; Adipose differentiation-related protein; ADRP; MGC10598; Perilipin-2; PLIN2; PLIN2_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q99541(PLIN2_HUMAN) >>Visit HPA database.

Gene(ID):

PLIN2(123)

Expression:

Q99541 PLIN2_HUMAN:

Milk lipid globules.

Sequence:

Q99541 PLIN2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASVAVDPQPSVVTRVVNLPLVSSTYDLMSSAYLSTKDQYPYLKSVCEMAENGVKTITSVAMTSALPIIQKLEPQIAVANTYACKGLDRIEERLPILNQPSTQIVANAKGAVTGAKDAVTTTVTGAKDSVASTITGVMDKTKGAVTGSVEKTKSVVSGSINTVLGSRMMQLVSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLSTKLHSRAYQQALSRVKEAKQKSQQTISQLHSTVHLIEFARKNVYSANQKIQDAQDKLYLSWVEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGVPQNIQDQAKHMGVMAGDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESLDDVMDYLVNNTPLNWLVGPFYPQLTESQNAQDQGAEMDKSSQETQRSEHKTH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Dog

100

Rabbit

Horse

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99541 As Substrate

Q99541

Site	PTM Type	Source
A2	Acetylation	Uniprot
K116	Ubiquitination	Uniprot
K140	Ubiquitination	Uniprot
S148	Phosphorylation	Uniprot
K153	Ubiquitination	Uniprot
S215	Phosphorylation	Uniprot
Y216	Phosphorylation	Uniprot
Y217	Phosphorylation	Uniprot
S222	Phosphorylation	Uniprot
S224	Phosphorylation	Uniprot
S237	Phosphorylation	Uniprot
S255	Phosphorylation	Uniprot
K273	Ubiquitination	Uniprot
S291	Phosphorylation	Uniprot
S362	Phosphorylation	Uniprot
K375	Ubiquitination	Uniprot

Research Backgrounds

Q99541_PLIN2_HUMAN

Function:

May be involved in development and maintenance of adipose tissue.

PTMs:

Acylated; primarily with C14, C16 and C18 fatty acids.

Subcellular Location:

Membrane>Peripheral membrane protein. Lipid droplet.

Tissue Specificity:

Milk lipid globules.

Family&Domains:

Belongs to the perilipin family.

Research Fields

· Organismal Systems > Endocrine system > PPAR signaling pathway.

References

1). Preventive Effects of Ilex Cornuta Aqueous Extract on High-Fat Diet-Induced Fatty Liver of Mice. Evidence-Based Complementary and Alternative Medicine (PubMed: 35432557)

Application: WB Species: Mouse Sample: liver

Figure 6 Effects of ICAE on the protein expression of PPARγ and ADRP in the liver of HFD-induced mice. The protein levels of PPARγ (a) and ADRP (b) were detected by immunoblot. Images were representative of three independent experiments, and average protein levels were quantified as ratios to β-actin, n = 5–6. ∗P < 0.05, ∗∗P < 0.01vs control; #P < 0.05, ####P < 0.0001vs. HFD group.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ADFP Antibody - #DF7603