Product: ADFP Antibody
Catalog: DF7603
Description: Rabbit polyclonal antibody to ADFP
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 48 kDa; 48kD(Calculated).
Uniprot: Q99541
RRID: AB_2841094

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(83%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ADFP Antibody detects endogenous levels of total ADFP.
RRID:
AB_2841094
Cite Format: Affinity Biosciences Cat# DF7603, RRID:AB_2841094.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADFP; Adipophilin; Adipose differentiation related protein; Adipose differentiation-related protein; ADRP; MGC10598; Perilipin-2; PLIN2; PLIN2_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q99541 PLIN2_HUMAN:

Milk lipid globules.

Sequence:
MASVAVDPQPSVVTRVVNLPLVSSTYDLMSSAYLSTKDQYPYLKSVCEMAENGVKTITSVAMTSALPIIQKLEPQIAVANTYACKGLDRIEERLPILNQPSTQIVANAKGAVTGAKDAVTTTVTGAKDSVASTITGVMDKTKGAVTGSVEKTKSVVSGSINTVLGSRMMQLVSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLSTKLHSRAYQQALSRVKEAKQKSQQTISQLHSTVHLIEFARKNVYSANQKIQDAQDKLYLSWVEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGVPQNIQDQAKHMGVMAGDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESLDDVMDYLVNNTPLNWLVGPFYPQLTESQNAQDQGAEMDKSSQETQRSEHKTH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
92
Horse
83
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99541 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K116 Ubiquitination
K140 Ubiquitination
S148 Phosphorylation
K153 Ubiquitination
S215 Phosphorylation
Y216 Phosphorylation
Y217 Phosphorylation
S222 Phosphorylation
S224 Phosphorylation
S237 Phosphorylation
S255 Phosphorylation
K273 Ubiquitination
S291 Phosphorylation
S362 Phosphorylation
K375 Ubiquitination

Research Backgrounds

Function:

May be involved in development and maintenance of adipose tissue.

PTMs:

Acylated; primarily with C14, C16 and C18 fatty acids.

Subcellular Location:

Membrane>Peripheral membrane protein. Lipid droplet.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Milk lipid globules.

Family&Domains:

Belongs to the perilipin family.

Research Fields

· Organismal Systems > Endocrine system > PPAR signaling pathway.

References

1). Preventive Effects of Ilex Cornuta Aqueous Extract on High-Fat Diet-Induced Fatty Liver of Mice. Evidence-Based Complementary and Alternative Medicine, 2022 (PubMed: 35432557)

Application: WB    Species: Mouse    Sample: liver

Figure 6 Effects of ICAE on the protein expression of PPARγ and ADRP in the liver of HFD-induced mice. The protein levels of PPARγ (a) and ADRP (b) were detected by immunoblot. Images were representative of three independent experiments, and average protein levels were quantified as ratios to β-actin, n = 5–6. ∗P < 0.05, ∗∗P < 0.01vs control; #P < 0.05, ####P < 0.0001vs. HFD group.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.