EPN2 Antibody - #AF0682

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Product: EPN2 Antibody
Catalog: AF0682
Description: Rabbit polyclonal antibody to EPN2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 68kDa; 68kD(Calculated).
Uniprot: O95208
RRID: AB_2834390

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Bovine(83%), Sheep(83%), Rabbit(92%), Dog(91%)
Clonality:
Polyclonal
Specificity:
EPN2 Antibody detects endogenous levels of total EPN2.
RRID:
AB_2834390
Cite Format: Affinity Biosciences Cat# AF0682, RRID:AB_2834390.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EHB21; EPN 2; Epn2; EPN2_HUMAN; EPS 15 interacting protein 2; EPS-15-interacting protein 2; Eps15 binding protein; Epsin 2; Epsin-2; Epsin2; KIAA1065; OTTHUMP00000065808; OTTHUMP00000065809; OTTHUMP00000065810; OTTHUMP00000065811; OTTHUMP00000065889;

Immunogens

Immunogen:
Uniprot:

O95208(EPN2_HUMAN) >>Visit HPA database.

Gene(ID):
EPN2(22905)
Expression:
O95208 EPN2_HUMAN:

Highest expression is found in brain. Detected at lower levels in lung and liver.

Description:
This gene encodes a protein which interacts with clathrin and adaptor-related protein complex 2, alpha 1 subunit. The protein is found in a brain-derived clathrin-coated vesicle fraction and localizes to the peri-Golgi region and the cell periphery. The protein is thought to be involved in clathrin-mediated endocytosis. Alternate splicing of this gene results in two transcript variants encoding different isoforms.
Sequence:
MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIADLTYNVVAFSEIMSMVWKRLNDHGKNWRHVYKALTLLDYLIKTGSERVAQQCRENIFAIQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERLKAERAQALKTKERMAQVATGMGSNQITFGRGSSQPNLSTSHSEQEYGKAGGSPASYHGSPEASLCPQHRTGAPLGQSEELQPLSQRHPFLPHLGLASRPNGDWSQPCLTCDRAARATSPRVSSELEQARPQTSGEEELQLQLALAMSREVAEQEERLRRGDDLRLQMALEESRRDTVKIPKKKEHGSLPQQTTLLDLMDALPSSGPAAQKAEPWGPSASTNQTNPWGGPAAPASTSDPWPSFGTKPAASIDPWGVPTGATVQSVPKNSDPWAASQQPASSAGKRASDAWGAVSTTKPVSVSGSFELFSNLNGTIKDDFSEFDNLRTSKKTAESVTSLPSQNNGTTSPDPFESQPLTVASSKPSSARKTPESFLGPNAALVNLDSLVTRPAPPAQSLNPFLAPGAPATSAPVNPFQVNQPQPLTLNQLRGSPVLGTSTSFGPGPGVESMAVASMTSAAPQPALGATGSSLTPLGPAMMNMVGSVGIPPSAAQATGTTNPFLL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
92
Dog
91
Pig
83
Bovine
83
Sheep
83
Horse
75
Chicken
67
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95208 As Substrate

Site PTM Type Enzyme
T2 Phosphorylation
S4 Phosphorylation
S5 Phosphorylation
K11 Ubiquitination
K69 Ubiquitination
Y83 Phosphorylation
K107 Ubiquitination
K117 Ubiquitination
K128 Ubiquitination
K135 Ubiquitination
K141 Ubiquitination
T150 Phosphorylation
S163 Phosphorylation
R170 Methylation
S172 Phosphorylation
S173 Phosphorylation
S178 Phosphorylation
S182 Phosphorylation
Y186 Phosphorylation
S192 Phosphorylation
S195 Phosphorylation
S199 Phosphorylation
S237 Phosphorylation
T249 Phosphorylation
T272 Phosphorylation
S273 Phosphorylation
S287 Phosphorylation
T316 Phosphorylation
K318 Ubiquitination
S327 Phosphorylation
S408 Phosphorylation
S420 Phosphorylation
K423 Acetylation
K423 Methylation
K423 Ubiquitination
S426 Phosphorylation
S486 Phosphorylation
S492 Phosphorylation
S504 Phosphorylation
T508 Phosphorylation
S570 Phosphorylation
S622 Phosphorylation
S628 Phosphorylation

Research Backgrounds

Function:

Plays a role in the formation of clathrin-coated invaginations and endocytosis.

PTMs:

Ubiquitinated.

Subcellular Location:

Cytoplasm. Cytoplasmic vesicle>Clathrin-coated vesicle.
Note: In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression is found in brain. Detected at lower levels in lung and liver.

Subunit Structure:

Binds EPS15 (By similarity). Interacts with ITSN1 (By similarity). Binds AP-2 and clathrin. Interacts with UBQLN2.

Family&Domains:

The NPF repeat domain is involved in EPS15 binding.

The DPW repeat domain is involved in AP-2 and clathrin binding.

Belongs to the epsin family.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

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