NCR1 Antibody | Affinity Biosciences

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Western blot analysis NKp46 using COLO205 whole cell lysates.

Product:	NCR1 Antibody
Catalog:	DF7599
Description:	Rabbit polyclonal antibody to NCR1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	34 kDa; 34kD(Calculated).
Uniprot:	O76036
RRID:	AB_2841090

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

NCR1 Antibody detects endogenous levels of total NCR1.

RRID:

AB_2841090
Cite Format: Affinity Biosciences Cat# DF7599, RRID:AB_2841090.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Activating NK receptor NK p46; CD335; FLJ99094; hNKp46; Ly94; Lymphocyte antigen 94; Lymphocyte antigen 94 homolog (activating NK receptor; NK p46); Lymphocyte antigen 94 homolog; Natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; NCR1; NCT1; NCTR1_HUMAN; NK cell activating receptor; NK cell-activating receptor; NK-p46; NKp46;

Immunogens

Immunogen:

Uniprot:

O76036(NCTR1_HUMAN) >>Visit HPA database.

Gene(ID):

NCR1(9437)

Expression:

O76036 NCTR1_HUMAN:

Selectively expressed by both resting and activated NK cells.

Sequence:

O76036 NCTR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSTLPALLCVGLCLSQRISAQQQTLPKPFIWAEPHFMVPKEKQVTICCQGNYGAVEYQLHFEGSLFAVDRPKPPERINKVKFYIPDMNSRMAGQYSCIYRVGELWSEPSNLLDLVVTEMYDTPTLSVHPGPEVISGEKVTFYCRLDTATSMFLLLKEGRSSHVQRGYGKVQAEFPLGPVTTAHRGTYRCFGSYNNHAWSFPSEPVKLLVTGDIENTSLAPEDPTFPADTWGTYLLTTETGLQKDHALWDHTAQNLLRMGLAFLVLVALVWFLVEDWLSRKRTRERASRASTWEGRRRLNTQTL

PTMs - O76036 As Substrate

O76036

Site	PTM Type	Source
Y84	Phosphorylation	Uniprot
S90	Phosphorylation	Uniprot
T292	Phosphorylation	Uniprot

Research Backgrounds

O76036_NCTR1_HUMAN

Function:

Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

PTMs:

N-glycosylated.

O-glycosylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Selectively expressed by both resting and activated NK cells.

Subunit Structure:

Interacts with CD247 and FCER1G.

Family&Domains:

Belongs to the natural cytotoxicity receptor (NCR) family.

Research Fields

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

References

1). Tumor cells impair immunological synapse formation via central nervous system-enriched metabolite. Cancer cell, 2024 (PubMed: 38821061) [IF=50.3]

2). pH-Triggered Copper-Free Click Reaction-Mediated Micelle Aggregation for Enhanced Tumor Retention and Elevated Immuno–Chemotherapy against Melanoma. ACS Applied Materials & Interfaces, 2021 (PubMed: 33834754) [IF=9.5]

Application: IF/ICC Species: mice Sample: NK cells

Figure 6. (A) Immunofluorescent CLSM images of B16F10 tumor slices at the end of treatment, NK cells were identified by the antimouse NKp46 antibody (red), and nuclei were visualized by DAPI (blue). Scale bar: 100 μm. (B) Flow cytometric analysis of intratumor NK cell populations identified by PE-conjugated antimouse NKp46. Error bars indicate SD (n = 3). ** and *** represent p < 0.01 and p < 0.001, respectively. (C) Typical immunohistochemical images of the B16F10 tumor sections stained with antibodies against IL-2 and GZMB. Scale bar: 100 μm. (D) Levels of IL-2, GZMB, and IFN-γ in tumor and (E) plasma analyzed by ELISA kits at the end of treatment. Error bars indicate SD (n = 3), *, **, and *** represent p < 0.05, p < 0.01, and p < 0.001, respectively. (F) Images of the expression level of intratumor phosphorylated Smad3 (pSmad3) and Smad3 by western blotting analysis, (a) PBS, (b) M-D@DOX, (c) free DOX/SIS3, (d) M-N@SIS3, (e) M@DOX/SIS3, and (f) MDN@DOX/SIS3. (G) Semiquantitative results of the expression levels of p-Smad3 and Smad3. Error bars indicate SD (n = 3), ** and *** represent p < 0.01 and p < 0.001, respectively.

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NCR1 Antibody - #DF7599