EPN1 Antibody - #AF0700
| Product: | EPN1 Antibody |
| Catalog: | AF0700 |
| Description: | Rabbit polyclonal antibody to EPN1 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Dog |
| Mol.Wt.: | 85kDa; 60kD(Calculated). |
| Uniprot: | Q9Y6I3 |
| RRID: | AB_2834389 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0700, RRID:AB_2834389.
Fold/Unfold
EH domain binding mitotic phosphoprotein; EH domain-binding mitotic phosphoprotein; EPN 1; Epn1; EPN1_HUMAN; EPS 15 interacting protein 1; EPS-15-interacting protein 1; Epsin 1; Epsin-1;
Immunogens
- Q9Y6I3 EPN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTSSLRRQMKNIVHNYSEAEIKVREATSNDPWGPSSSLMSEIADLTYNVVAFSEIMSMIWKRLNDHGKNWRHVYKAMTLMEYLIKTGSERVSQQCKENMYAVQTLKDFQYVDRDGKDQGVNVREKAKQLVALLRDEDRLREERAHALKTKEKLAQTATASSAAVGSGPPPEAEQAWPQSSGEEELQLQLALAMSKEEADQPPSCGPEDDAQLQLALSLSREEHDKEERIRRGDDLRLQMAIEESKRETGGKEESSLMDLADVFTAPAPAPTTDPWGGPAPMAAAVPTAAPTSDPWGGPPVPPAADPWGGPAPTPASGDPWRPAAPAGPSVDPWGGTPAPAAGEGPTPDPWGSSDGGVPVSGPSASDPWTPAPAFSDPWGGSPAKPSTNGTTAAGGFDTEPDEFSDFDRLRTALPTSGSSAGELELLAGEVPARSPGAFDMSGVRGSLAEAVGSPPPAATPTPTPPTRKTPESFLGPNAALVDLDSLVSRPGPTPPGAKASNPFLPGGGPATGPSVTNPFQPAPPATLTLNQLRLSPVPPVPGAPPTYISPLGGGPGLPPMMPPGPPAPNTNPFLL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y6I3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K11 | Ubiquitination | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| Y101 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| S382 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T412 | Phosphorylation | Uniprot | |
| T416 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| S435 | Phosphorylation | Uniprot | |
| S442 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| T460 | Phosphorylation | Uniprot | |
| T462 | Phosphorylation | Uniprot | |
| T464 | Phosphorylation | Uniprot | |
| T467 | Phosphorylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| T470 | Phosphorylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| T494 | Phosphorylation | Uniprot | |
| S501 | Phosphorylation | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| Y548 | Phosphorylation | Uniprot |
Research Backgrounds
Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations (By similarity). Regulates receptor-mediated endocytosis.
Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.
Ubiquitinated.
Cytoplasm. Cell membrane>Peripheral membrane protein. Nucleus. Membrane>Clathrin-coated pit.
Note: Associated with the cytoplasmic membrane at sites where clathrin-coated pits are forming. Colocalizes with clathrin and AP-2 in a punctate pattern on the plasma membrane. Detected in presynaptic nerve terminals and in Golgi stacks. May shuttle to the nucleus when associated with ZBTB16/ZNF145 (By similarity).
Monomer. Binds clathrin, ZBTB16/ZNF145 and ITSN1 (By similarity). Binds ubiquitinated proteins (By similarity). Binds REPS2, EPS15, AP2A1 and AP2A2. Interacts with RALBP1 in a complex also containing NUMB and TFAP2A during interphase and mitosis. Interacts with AP2B1. Interacts with UBQLN2.
The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP2A2 and clathrin binding.
The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction with the AP-2 complex subunit AP2B1.
Belongs to the epsin family.
Research Fields
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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