CD11c Antibody | Affinity Biosciences

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DF7585 at 1/100 staining Human ovarian cancer by IHC-P.

Product:	CD11c Antibody
Catalog:	DF7585
Description:	Rabbit polyclonal antibody to CD11c
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	130~150kDa; 128kD(Calculated).
Uniprot:	P20702
RRID:	AB_2841076

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(91%), Bovine(91%), Horse(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

CD11c Antibody detects endogenous levels of total CD11c.

RRID:

AB_2841076
Cite Format: Affinity Biosciences Cat# DF7585, RRID:AB_2841076.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

95 alpha chain; 95; CD 11c; CD11 antigen like family member C; CD11 antigen-like family member C; CD11c; CD11c antigen; Complement component 3 receptor 4 subunit; CR4; Integrin alpha X; Integrin alpha X chain; Integrin alpha-X; Integrin aX; Integrin subunit alpha X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit; ITAX_HUMAN; ITGAX; LEU M5 alpha subunit; Leu M5; Leukocyte adhesion glycoprotein p150 95 alpha chain; Leukocyte adhesion glycoprotein p150; Leukocyte adhesion receptor p150 95; Leukocyte adhesion receptor p150; Leukocyte surface antigen p150 95 alpha subunit; Leukocyte surface antigen p150 alpha subunit; Myeloid membrane antigen alpha subunit; p150 95 integrin alpha chain; p150; p150/95; SLEB6;

Immunogens

Immunogen:

Uniprot:

P20702(ITAX_HUMAN) >>Visit HPA database.

Gene(ID):

ITGAX(3687)

Expression:

P20702 ITAX_HUMAN:

Predominantly expressed in monocytes and granulocytes.

Sequence:

P20702 ITAX_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTRTRAALLLFTALATSLGFNLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMAQEGFSAVFTPDGPVLGAVGSFTWSGGAFLYPPNMSPTFINMSQENVDMRDSYLGYSTELALWKGVQSLVLGAPRYQHTGKAVIFTQVSRQWRMKAEVTGTQIGSYFGASLCSVDVDSDGSTDLVLIGAPHYYEQTRGGQVSVCPLPRGWRRWWCDAVLYGEQGHPWGRFGAALTVLGDVNGDKLTDVVIGAPGEEENRGAVYLFHGVLGPSISPSHSQRIAGSQLSSRLQYFGQALSGGQDLTQDGLVDLAVGARGQVLLLRTRPVLWVGVSMQFIPAEIPRSAFECREQVVSEQTLVQSNICLYIDKRSKNLLGSRDLQSSVTLDLALDPGRLSPRATFQETKNRSLSRVRVLGLKAHCENFNLLLPSCVEDSVTPITLRLNFTLVGKPLLAFRNLRPMLAADAQRYFTASLPFEKNCGADHICQDNLGISFSFPGLKSLLVGSNLELNAEVMVWNDGEDSYGTTITFSHPAGLSYRYVAEGQKQGQLRSLHLTCDSAPVGSQGTWSTSCRINHLIFRGGAQITFLATFDVSPKAVLGDRLLLTANVSSENNTPRTSKTTFQLELPVKYAVYTVVSSHEQFTKYLNFSESEEKESHVAMHRYQVNNLGQRDLPVSINFWVPVELNQEAVWMDVEVSHPQNPSLRCSSEKIAPPASDFLAHIQKNPVLDCSIAGCLRFRCDVPSFSVQEELDFTLKGNLSFGWVRQILQKKVSVVSVAEITFDTSVYSQLPGQEAFMRAQTTTVLEKYKVHNPTPLIVGSSIGGLLLLALITAVLYKVGFFKRQYKEMMEEANGQIAPENGTQTPSPPSEK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Rabbit

100

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P20702 As Substrate

P20702

Site	PTM Type	Source
N61	N-Glycosylation	Uniprot
N392	N-Glycosylation	Uniprot
S668	Phosphorylation	Uniprot
S673	Phosphorylation	Uniprot
S674	Phosphorylation	Uniprot
N697	N-Glycosylation	Uniprot
N735	N-Glycosylation	Uniprot
N899	N-Glycosylation	Uniprot
S1158	Phosphorylation	Uniprot

Research Backgrounds

P20702_ITAX_HUMAN

Function:

Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Tissue Specificity:

Predominantly expressed in monocytes and granulocytes.

Subunit Structure:

Heterodimer of an alpha and a beta subunit. Alpha-X associates with beta-2.

Family&Domains:

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Belongs to the integrin alpha chain family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

References

1). Multifunctional 3D-printed scaffolds eradiate orthotopic osteosarcoma and promote osteogenesis via microwave thermo-chemotherapy combined with immunotherapy. Biomaterials, 2023 (PubMed: 37506512) [IF=14.0]

2). A mosquito salivary protein-driven influx of myeloid cells facilitates flavivirus transmission. The EMBO journal, 2024 (PubMed: 38378891) [IF=11.4]

3). Anti-inflammatory effect of piperine ameliorates insulin resistance in monosodium glutamate–treated obese mice. METABOLISM-CLINICAL AND EXPERIMENTAL, 2020 (PubMed: 19800637) [IF=9.8]

Application: WB Species: Mouse Sample: RAW264.7 cells

Figure 9. Effects of piperine on LPS-induced M1-like polarization in RAW264.7 cells. RAW264.7 cells were pretreated with piperine at 20-80 μM for 12 h and then stimulated by LPS (1 μg/ml) for 24 h. (a-c) The mRNA levels of CD11c, IL-1β and TNF-α were analyzed by qPCR. (d) The IL-1β level in cell supernatant was measured by ELISA. (e-f) The protein levels of TLR-4, CD11c and IL-1β were tested by Western Blotting. Data are expressed as mean±SD, n=3. ####p

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For Research Use Only.
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CD11c Antibody - #DF7585