MCP1 Antibody - #DF7577

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Product: MCP1 Antibody
Catalog: DF7577
Description: Rabbit polyclonal antibody to MCP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse
Mol.Wt.: 14 kDa; 11kD(Calculated).
Uniprot: P13500
RRID: AB_2841069

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(83%)
Clonality:
Polyclonal
Specificity:
MCP1 Antibody detects endogenous levels of total MCP1.
RRID:
AB_2841069
Cite Format: Affinity Biosciences Cat# DF7577, RRID:AB_2841069.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C-C motif chemokine 2; CCL2; CCL2_HUMAN; Chemokine (C C motif) ligand 2; GDCF 2; GDCF-2; GDCF2; HC11; HSMCR30; JE; MCAF; MCP 1; MCP-1; MCP1; MGC9434; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig je); Small inducible cytokine A2; Small inducible cytokine subfamily A (Cys Cys), member 2; Small-inducible cytokine A2; SMC CF; SMC-CF; SMCCF;

Immunogens

Immunogen:

A synthesized peptide derived from human MCP1, corresponding to a region within the internal amino acids.

Uniprot:

P13500(CCL2_HUMAN) >>Visit HPA database.

Gene(ID):
CCL2(6347)
Expression:
P13500 CCL2_HUMAN:

Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level) (PubMed:23765988). Expressed in monocytes (PubMed:2513477).

Sequence:
MKVSAALLCLLLIAATFIPQGLAQPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
83
Pig
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

PTMs:

Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

N-Glycosylated.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level). Expressed in monocytes.

Subunit Structure:

Monomer or homodimer; in equilibrium. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans.

Family&Domains:

Belongs to the intercrine beta (chemokine CC) family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

References

1). Outer membrane vesicles derived from gut microbiota mediate tubulointerstitial inflammation: a potential new mechanism for diabetic kidney disease. Theranostics, 2023 (PubMed: 37554279) [IF=12.4]

Application: IHC    Species: Rat    Sample:

Figure 1 Gut microbiota depletion attenuates systemic and tubulointerstitial inflammation in diabetic rats. Gut microbiota of DM rats was depleted by treatment with broad-spectrum antibiotics administered in the drinking water (DM+AB). (A) Serum inflammatory cytokines, including IL-1β, TNF-α, MCP-1 and IL-6, were measured by ELISA (n=4). (B) Tubulointerstitial inflammation was detected by immunohistochemical staining (scale bar, 500 μm and 100 μm, original magnification × 200). (C-D) Expression levels of inflammatory proteins in renal cortex were detected by Western blot analysis and quantified (n=4-5). (E-F) Histopathological injuries of tubulointerstitium were evaluated by PAS staining (scale bar, 500 μm and 100 μm, original magnification × 200) and quantified (n=5).

Application: WB    Species: Rat    Sample:

Figure 1 Gut microbiota depletion attenuates systemic and tubulointerstitial inflammation in diabetic rats. Gut microbiota of DM rats was depleted by treatment with broad-spectrum antibiotics administered in the drinking water (DM+AB). (A) Serum inflammatory cytokines, including IL-1β, TNF-α, MCP-1 and IL-6, were measured by ELISA (n=4). (B) Tubulointerstitial inflammation was detected by immunohistochemical staining (scale bar, 500 μm and 100 μm, original magnification × 200). (C-D) Expression levels of inflammatory proteins in renal cortex were detected by Western blot analysis and quantified (n=4-5). (E-F) Histopathological injuries of tubulointerstitium were evaluated by PAS staining (scale bar, 500 μm and 100 μm, original magnification × 200) and quantified (n=5).
2). Micheliolide Ameliorates Diabetic Kidney Disease by Inhibiting Mtdh-mediated Renal Inflammation in Type 2 Diabetic db/db Mice. PHARMACOLOGICAL RESEARCH, 2019 (PubMed: 31669149) [IF=9.3]
3). Interfering microRNA-410 attenuates atherosclerosis via the HDAC1/KLF5/IKBα/NF-κB axis. Molecular Therapy - Nucleic Acids, 2021 (PubMed: 33981482) [IF=8.8]

Application: WB    Species: Mouse    Sample: aorta tissues

Figure 2 miR-40 inhibition suppresses the initiation of atherosclerosis in mice (A) Detection of miR-410 expression level in aorta tissues. (B) Arterial impairment and inflammation. (C) The area of aorta impairment. (D) Serum TNF-α, IL-1, and IL-6 levels in mice. (E) VCAM-1, ICAM-1, and MCP-1 expression in aorta tissues; ∗p < 0.05. Measured data are described as mean ± standard deviation, analyzed using independent samples t test between two groups; n = 10.
4). Inhibition of miR-155-5p Exerts Anti-Fibrotic Effects in Silicotic Rats by Regulating Meprin α. Molecular Therapy-Nucleic Acids, 2019 (PubMed: 31877411) [IF=8.8]

Application: WB    Species: Rat    Sample: RAW264.7 cells

Figure 4. miR-155-5p Negatively Regulates Mep1a (A) Protein expression of meprin a, pro-COL I, TGF-b1, a-SMA, and MCP-1 was detected by western blotting and quantified. (B) Levels of miR-155-5p and Mep1a in rat lungs. Data are presented as the mean ± SD. n = 5 per group. (C and D) Protein (C) and (D) mRNA (D) levels of meprin a in RAW264.7 cells treated with agomiR-155-5p or antamiR-155-5p. (E) Luciferase reporter assay demonstrating Mep1a was a target of miR-155-5p. Data are presented as the mean ± SD. n = 3 per group
5). HIF-1α/JMJD1A signaling regulates inflammation and oxidative stress following hyperglycemia and hypoxia-induced vascular cell injury. CELLULAR & MOLECULAR BIOLOGY LETTERS, 2021 (PubMed: 34479471) [IF=8.3]

Application: WB    Species: human    Sample: HUVECs

Fig. 3 | Efects of inhibition of HIF-1α on expression of infammation after hyperglycemic and hypoxia.b Cells were treated with KC7F2 (10 µM) or si-HIF-1α for 48 h following combined stimulation, and IL-6, IL-8, ICAM-1, MCP-1, and c HIF-1α levels were analyzed. The relative density of IL-6, IL-8,ICAM-1, and MCP-1 (d) was normalized according to GAPDH expression.

Application: WB    Species: Human    Sample: HUVECs

Fig. 3 Effects of inhibition of HIF-1α on expression of inflammation after hyperglycemic and hypoxia. a Cells were treated with glucose (25 mM) or with combined exposure to high glucose and hypoxia for 6, 12, 24, and 48 h. Exposure to glucose alone or combined stimulation with hypoxia significantly increased gene expression of HIF-1α. b Cells were treated with KC7F2 (10 µM) or si-HIF-1α for 48 h following combined stimulation, and IL-6, IL-8, ICAM-1, MCP-1, and c HIF-1α levels were analyzed. The relative density of IL-6, IL-8, ICAM-1, and MCP-1 (d) was normalized according to GAPDH expression. Importantly, the downregulation of HIF-1α decreased the secretion of IL-6, IL-8, ICAM-1 and MCP-1 based on ELISA and qRT-PCR assays (e–f). n = 3; *p < 0.05 and **p < 0.01 vs. DMSO. DMSO-treated cells, DMSO; HIF-1α inhibitors KC7F2 (10 µM)-treated cells, KC7F2 (10 µM); si-HIF-1α, small interfering RNA HIF-1α; HG, high glucose; HG + Hypoxia, combined stimulus with high glucose and hypoxia; NG, control
6). A bioactive xyloglucan polysaccharide hydrogel mechanically enhanced by Pluronic F127 micelles for promoting chronic wound healing. International journal of biological macromolecules, 2024 (PubMed: 39047998) [IF=8.2]
7). Acetyl-CoA synthetase 2 promotes diabetic renal tubular injury in mice by rewiring fatty acid metabolism through SIRT1/ChREBP pathway. Acta Pharmacologica Sinica, 2023 (PubMed: 37770579) [IF=8.2]
8). Natural flavonol fisetin attenuated hyperuricemic nephropathy via inhibiting IL-6/JAK2/STAT3 and TGF-β/SMAD3 signaling. PHYTOMEDICINE, 2021 (PubMed: 33994251) [IF=7.9]

Application: WB    Species: Mice    Sample: kidneys

Fig. 3. Effects of fisetin on proinflammatory production in the kidneys of HN mice. (A) The mRNA expression levels of IL-6, TNF-α, and MCP-1 in renal tissue were analyzed by RT-PCR. (B) The protein expression levels of IL-6, TNF-α, and MCP-1were detected by western blot. (C-E) The ratio of IL-6, TNF-α, and MCP-1 to GAPDH was calculated. Data are expressed as mean ± SEM (n = 6). **** p < 0.0001 vs. control; #### p < 0.0001 vs. model. The ns. means no significance.
9). Kaempferol alleviates calcium oxalate crystal-induced renal injury and crystal deposition via regulation of the AR/NOX2 signaling pathway. PHYTOMEDICINE, 2021 (PubMed: 33852977) [IF=7.9]

Application: WB    Species: Human    Sample: HK-2 cells

Fig. 4. Kaempferol inhibited renal oxidative stress and inflammation via regulating NOX2 expression in vitro. (a-b) The intracellular ROS level was detected by flow cytometry after loading with DCFH-DA. Moreover, (c) H2O2 expression, as well as (d) GSH, SOD and MDA levels were examined. Afterwards, (e-l) the mRNA levels of IL-1β, IL-6, TNF-α, IL-10, IL-4, Arg1, OPN and CD44 were measured by RT-qPCR, and (m) The RT-qPCR of NOX subunits was analyzed after the exposure of COM crystals (400 μg/ml) or 0.5 mM oxalate with or without kaempferol (40 μM) for 12 h. (n, o) The NOX2, iNOS, p-NFκB-p65 and MCP-1 expressions in HK-2 cells were measured by WB. Data were expressed as the fold changes of the experimental group to the NC group (except part b) and were represented as means ± SD of three independent experiments with different cell passages on different days. ∆ p < 0.05; ∆∆ p < 0.01; * p < 0.05 vs. NC group; # p < 0.05 vs. COM group; ** p < 0.05 as pairwise comparisons of three groups among COM + Kae (10 μM), COM + Kae (20 μM), and COM + Kae (40 μM).
10). Pyrogallol enhances therapeutic effect of human umbilical cord mesenchymal stem cells against LPS-mediated inflammation and lung injury via activation of Nrf2/HO-1 signaling. Free radical biology & medicine, 2022 (PubMed: 36028178) [IF=7.4]
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